ABSTRACT
BACKGROUND: This study investigates the association between polymorphism in the E-cadherin/CDH1 promoter region and the risk and progression of oral cancer. METHODS: Genetic polymorphisms of CDH1-160 and -347 were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 347 noncancer controls and in 251 patients with oral cancer. RESULTS: The statistical analysis showed that subjects with at least 1 varied GA allele of CDH1-347 polymorphic genotypes or combinations of the CDH1-160 CA/-347 GGA, CDH1-160 CC/-347 GGA, or CDH1-160 CC/-347 GAGA genotypes had a significantly higher risk, whereas subjects with CDH1-160 C/A or A/A had a significantly lower risk of developing oral cancer than those with wild-type genotypes. Furthermore, elderly patients with the CDH1-347 G/GA or GA/GA genotype were associated with a higher incidence in lymph node metastasis than were those with the G/G genotype. CONCLUSIONS: These results suggest that CDH1-347 polymorphisms are associated with increased risks of oral cancer, and may be a predictive factor for tumor lymph node metastasis.
Subject(s)
Cadherins/genetics , Genetic Predisposition to Disease/etiology , Mouth Neoplasms/genetics , Mouth Neoplasms/pathology , Polymorphism, Genetic , Promoter Regions, Genetic/genetics , Adult , Amplified Fragment Length Polymorphism Analysis , Antigens, CD , Asian People/genetics , Case-Control Studies , Cohort Studies , Female , Humans , Male , Middle Aged , Mouth Neoplasms/ethnology , TaiwanABSTRACT
BACKGROUND: The purpose of this study was to evaluate the influence of genetic polymorphisms of interleukin (IL)-23 and the IL-23 receptor (IL-23R) on the susceptibility to oral cancer. METHODS: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to measure polymorphisms of these genes in 240 controls and 240 patients with oral cancer. RESULTS: Individuals with at least 1 varied C allele of rs10889677 (IL-23R polymorphism) had a 1.553-fold risk (95% confidence interval [CI], 1.073-2.241) of developing oral cancer compared with patients with the wild-type A/A homozygote. Patients with oral cancer with at least 1 varied C allele of rs10889677 had a 1.931-fold risk of tumor lymph node metastasis compared with patients with the C/C homozygote. CONCLUSION: The varied C allele of the IL-23R gene may be considered a factor contributing to increased susceptibility and may be a predictive factor for tumor lymph node metastasis in Taiwanese with oral cancer.
