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1.
Curr Microbiol ; 81(3): 88, 2024 Feb 05.
Article in English | MEDLINE | ID: mdl-38311656

ABSTRACT

Antimicrobial peptides (AMPs) stand as a promising alternative to conventional pesticides, leveraging a multifaceted approach to combat plant pathogens. This study focuses on identifying and characterizing the AMP produced by Lactiplantibacillus argentoratensis strain IT, demonstrating potent antibacterial activity against various harmful microorganisms. Evaluation of AMPs' antibacterial activity was conducted through an agar well diffusion assay, a reliable method for assessing secondary metabolite antimicrobial efficacy. The study unveils the antimicrobial potential of the purified extract obtained from Lactiplantibacillus argentoratensis IT, isolated from goat milk. Notably, the AMP exhibited robust antibacterial activity against phytopathogens affecting solanaceous crops, including the Gram-negative Ralstonia solanacearum. Expression conditions and purification methods were optimized to identify the peptide's mass and sequence, utilizing LC-MS and SDS-PAGE. This paper underscores the application potential of Lactiplantibacillus spp. IT as a biocontrol agent for managing bacterial infectious diseases in plants. Results indicate optimal AMP production at 37 °C, with a culture broth pH of 5 during fermentation. The obtained peptide sequence corresponded to peaks at 842.5 and 2866.4 m/z ratio, with a molecular weight of approximately 5 kDa according to tricine SDS-PAGE analysis. In conclusion, this study lays the foundation for utilizing Lactiplantibacillus spp. IT derived AMPs in plant biocontrol strategies, showcasing their efficacy against bacterial phytopathogens. These findings contribute valuable insights for advancing sustainable agricultural practices.


Subject(s)
Anti-Infective Agents , Peptides , Bacteria , Anti-Bacterial Agents , Amino Acid Sequence , Plants/microbiology
2.
Int J Surg Case Rep ; 114: 109045, 2024 Jan.
Article in English | MEDLINE | ID: mdl-38039569

ABSTRACT

INTRODUCTION AND IMPORTANCE: Renal cell carcinoma is the most lethal malignancy of urinary tract. Invasion of right lobe of liver by Renal cell carcinoma is rare and possess a treatment challenge. Simultaneous nephrectomy with right hepatectomy has been proposed as a part of multi-modality treatment approach. But its safety and feasibility is not well established. CASE PRESENTATION: We herein discuss a case of 30-year old female patient who underwent simultaneous nephrectomy with right hepatectomy along with single peritoneal metastasectomy for a huge Renal cell carcinoma of right kidney and infiltrating the right lobe of liver. Intra-operatively a single peritoneal nodule was present which came positive for malignancy on frozen section. Considering young age, good performance status and oligometastatic disease definitive procedure in the form of combined right nephrectomy and right hepatectomy was performed. She was discharged from the hospital on 6th post-operative day with an uneventful post-operative course. CLINICAL DISCUSSION: The patients with locally advanced Renal cell carcinoma with involvement of adjacent organs require en block surgical resection in combination with targeted therapy and immunotherapy. The surgical management of patients with direct liver infiltration requires a right nephrectomy with some form of liver resection based on the extent of liver involvement to achieve a margin negative resection. In our case a plan of formal right hepatectomy was made as the tumor was infiltrating into segment VI, VII, and VIII. CONCLUSION: The combined nephrectomy and right hepatectomy is safe and feasible for this type of huge RCC invading right hepatic lobe.

4.
J Minim Access Surg ; 19(3): 408-413, 2023.
Article in English | MEDLINE | ID: mdl-37282436

ABSTRACT

Background: Laparoscopic Heller myotomy (LHM) can be performed by blunt dissection technique (BDT). Only a few studies have assessed long-term outcomes and relief of dysphagia following LHM. The study reviews our long-term experience following LHM by BDT. Methods: This retrospective study was analysed from a prospectively maintained database (from 2013 to 2021) of a single unit of the Department of Gastrointestinal Surgery at G. B. Pant Institute of Postgraduate Medical Education and Research, New Delhi. The myotomy was performed by BDT in all patients. A fundoplication was added in selected patients. Post-operative Eckardt score >3 was considered treatment failure. Results: A total of 100 patients underwent surgery during the study period. Of them, 66 patients underwent LHM, 27 underwent LHM with Dor fundoplication and 7 underwent LHM with Toupet fundoplication. The median length of myotomy was 7 cm. The mean operative time was 77 ± 29.27 min and the mean blood loss of 28.05 ± 16.06 ml. Five patients had intraoperative oesophageal perforation. The median length of hospital stay was 2 days. There was no hospital mortality. The post-operative integrated relaxation pressure (IRP) was significantly lower than the mean pre-operative IRP (9.78 vs. 24.77). Eleven patients developed treatment failure, of which ten patients presented with recurrence of dysphagia. There was no difference in symptom-free survival amongst various types of achalasia cardia (P = 0.816). Conclusion: LHM performed by BDT has a 90% success rate. Complication using this technique is rare, and recurrence post-surgery can be managed with endoscopic dilatation.

5.
Front Plant Sci ; 14: 1155715, 2023.
Article in English | MEDLINE | ID: mdl-37293679

ABSTRACT

Rapid urbanization and globalization demand increasing agricultural productivity. Soil nutrient supply capacity is continuously decreasing due to soil erosion, degradation, salt deposition, undesired element, metal deposition, water scarcity, and an uneven nutrient delivery system. Rice cultivation requires a large amount of water which is becoming detrimental due to these activities. There is a need to increase its productivity. Microbial inoculants are becoming increasingly important in achieving sustainable agricultural production systems. The current study was conducted to investigate the interaction between the root endophytic fungus Serendipita indica (S. indica) and the actinobacterium Zhihengliuella sp. ISTPL4 (Z. sp. ISTPL4) and their synergistic effects on the growth of rice (Oryza sativa L). Both S. indica and Z. sp. ISTPL4 showed positive interactions. Growth of S. indica was observed at different days after Z. sp. ISTPL4 inoculation, and stimulated growth of S. indica was observed when Z. sp. ISTPL4 was inoculated at 5 dafi (days after fungal inoculation). Z. sp. ISTPL4 promoted the growth of S. indica as it increased spore germination. Furthermore, confocal and scanning electron microscopy (SEM) analyses showed a 27% increase in the spore size of S. indica in the presence of Z. sp. ISTPL4. In a liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis increased production of alanine and glutamic acid was observed in their sequential co-culture as compared with individual cultures. Sequential inoculation of S. indica and Z. sp. ISTPL4 significantly increased the biochemical and physical characteristics of rice as compared with their individual inoculum. Biochemical parameters such as chlorophyll content, total soluble sugar, and flavonoid content in the rice increased by up to 57%, 47%, and 39%, respectively, in the presence of the combined inoculum of S. indica and Z. sp. ISTPL4. This will be the first study, to the best of our knowledge, which shows the fungus and actinobacterium interaction and their synergistic roles in the growth promotion of rice. Furthermore, this novel combination can also be used to boost the growth of other crops to increase the agricultural yield.

6.
Cureus ; 14(10): e30594, 2022 Oct.
Article in English | MEDLINE | ID: mdl-36420237

ABSTRACT

Background Patients with gallbladder carcinoma (GBC) and jaundice have a poor prognosis. The surgical management of these patients is controversial. There is a dearth of studies comparing curative surgical resection (CR) versus non-curative resection/palliation (NCR) in patients with GBC and jaundice. Hence, this study aimed to compare the outcomes between CR and NCR in these patients. Methodology This was a retrospective study on patients with GBC and jaundice managed by a single surgical unit at a tertiary care center in northern India from May 2009 to March 2021. These patients were grouped into CR or NCR. The clinical demographical profile and overall survival (OS) were compared between the groups. Results A total of 82 patients with GBC and jaundice were managed during the study period. The final study cohort included 59 patients (CR, n = 34; NCR, n = 25) after excluding patients with metastatic disease (n = 23). Common bile duct infiltration was seen in 61.7% and 84% of CR and NCR patients, respectively (p = 0.062). The overall tumor-node-metastasis staging between the two groups was similar (p = 0.296). The median OS of CR was significantly better in CR than NCR (20 months vs. six months; p = 0.001). The median OS was better in CR than NCR patients who received systemic chemotherapy (22 vs. 12 months; p = 0.001) or did not receive chemotherapy (14 months vs. three months; p = 0.001). Conclusions Patients with GBC and jaundice have better significant survival after CR than NCR alone.

7.
Plants (Basel) ; 11(18)2022 Sep 13.
Article in English | MEDLINE | ID: mdl-36145787

ABSTRACT

Rice production needs to be sustained in the coming decades, as the changeable climatic conditions are becoming more conducive to disease outbreaks. The majority of rice diseases cause enormous economic damage and yield instability. Among them, rice blast caused by Magnaportheoryzae is a serious fungal disease and is considered one of the major threats to world rice production. This pathogen can infect the above-ground tissues of rice plants at any growth stage and causes complete crop failure under favorable conditions. Therefore, management of blast disease is essentially required to sustain global food production. When looking at the drawback of chemical management strategy, the development of durable, resistant varieties is one of the most sustainable, economic, and environment-friendly approaches to counter the outbreaks of rice blasts. Interestingly, several blast-resistant rice cultivars have been developed with the help of breeding and biotechnological methods. In addition, 146 R genes have been identified, and 37 among them have been molecularly characterized to date. Further, more than 500 loci have been identified for blast resistance which enhances the resources for developing blast resistance through marker-assisted selection (MAS), marker-assisted backcross breeding (MABB), and genome editing tools. Apart from these, a better understanding of rice blast pathogens, the infection process of the pathogen, and the genetics of the immune response of the host plant are very important for the effective management of the blast disease. Further, high throughput phenotyping and disease screening protocols have played significant roles in easy comprehension of the mechanism of disease spread. The present review critically emphasizes the pathogenesis, pathogenomics, screening techniques, traditional and molecular breeding approaches, and transgenic and genome editing tools to develop a broad spectrum and durable resistance against blast disease in rice. The updated and comprehensive information presented in this review would be definitely helpful for the researchers, breeders, and students in the planning and execution of a resistance breeding program in rice against this pathogen.

8.
Asian J Pharm Sci ; 16(4): 397-418, 2021 Jul.
Article in English | MEDLINE | ID: mdl-34703491

ABSTRACT

Nanotechnology has changed the entire paradigm of drug targeting and has shown tremendous potential in the area of cancer therapy due to its specificity. In cancer, several targets have been explored which could be utilized for the better treatment of disease. Mitochondria, the so-called powerhouse of cell, portrays significant role in the survival and death of cells, and has emerged as potential target for cancer therapy. Direct targeting and nanotechnology based approaches can be tailor-made to target mitochondria and thus improve the survival rate of patients suffering from cancer. With this backdrop, in present review, we have reemphasized the role of mitochondria in cancer progression and inhibition, highlighting the different targets that can be explored for targeting of disease. Moreover, we have also summarized different nanoparticulate systems that have been used for treatment of cancer via mitochondrial targeting.

9.
Curr Opin Pharmacol ; 54: 109-120, 2020 10.
Article in English | MEDLINE | ID: mdl-33053492

ABSTRACT

Multiple drug resistance (MDR) is a significant challenge in the treatment of cancer using chemotherapy. There are numerous reasons and mechanisms that are responsible for the development of MDR in cancer tissues. Further, exosomes and its constituents also play a vital role in limiting the efficacy of chemotherapeutic agents. Exosomes are well known for their role in developing resistance in addition to promoting tumor advancement and metastasis. This review discusses the role of exosomes in the development of drug resistance along with their allied mechanisms. This review also discusses the upregulation and downregulation of various exosomal components, which can be effectively employed as diagnostic biomarkers in the treatment of cancer. The essential applications of exosomes to treat drug-resistant cancer have also been discussed.


Subject(s)
Antineoplastic Agents/therapeutic use , Drug Resistance, Multiple , Drug Resistance, Neoplasm , Exosomes , Neoplasms/drug therapy , Animals , Biomarkers , Humans , Neoplasms/diagnosis , Nucleic Acids , Proteins
10.
Ecotoxicol Environ Saf ; 186: 109741, 2019 Dec 30.
Article in English | MEDLINE | ID: mdl-31600651

ABSTRACT

Piriformospora indica is known for plant growth promotion and abiotic stress alleviation potential in several agricultural crops. However, a systemic analysis is warranted to explore potential application of this important fungus to augment heavy metal tolerance in rice. The present study explores potential of P. indica in ameliorating the effect of cadmium (Cd) stress in rice cultivars N22 and IR64. Seedlings inoculated with P. indica recorded significantly higher root-shoot length and biomass as compared to non-inoculated plants under control and Cd stress, respectively. Moreover, P. indica inoculated stressed roots accumulated more Cd as compared to non-inoculated stressed roots in both the varieties. Interestingly, cell death and reactive oxygen species (ROS) accumulation were significantly lower in the inoculated plant roots as compare with non-inoculated roots under Cd stress. The results emphasized significantly higher accumulation of Cd in fungal spores could reduce ROS accumulation in root cells resulting in lower cell death.


Subject(s)
Basidiomycota/physiology , Cadmium/toxicity , Oryza/microbiology , Oxidative Stress , Plant Roots/microbiology , Basidiomycota/metabolism , Biomass , Cadmium/metabolism , Cell Death/drug effects , Oryza/drug effects , Oryza/growth & development , Oryza/metabolism , Oxidative Stress/drug effects , Plant Roots/drug effects , Plant Roots/growth & development , Plant Roots/metabolism , Reactive Oxygen Species/metabolism , Seedlings/growth & development , Seedlings/metabolism
11.
12.
BMC Res Notes ; 12(1): 131, 2019 Mar 13.
Article in English | MEDLINE | ID: mdl-30867027

ABSTRACT

OBJECTIVE: Geographic expansion of dengue incidence has drawn a global interest to identify the influential factors that instigate the spread of this disease. The objective of this study was to find the environmental factors linked to dengue incidence in a dengue epidemic area of Nepal by negative binomial models using climatic factors from 2010 to 2017. RESULTS: Minimum temperature at lag 2 months, maximum temperature and relative humidity without lag period significantly affected dengue incidence. Rainfall was not associated with dengue incidence in Chitwan district of Nepal. The incident rate ratio (IRR) of dengue case rise by more than 1% for every unit increase in minimum temperature at lag 2 months, maximum temperature and relative humidity, but decrease by .759% for maximum temperature at lag 3 months. Considering the effect of minimum temperature of previous months on dengue incidence, the vector control and dengue management program should be implemented at least 2 months ahead of dengue outbreak season.


Subject(s)
Dengue/epidemiology , Epidemics/statistics & numerical data , Rain , Seasons , Temperature , Humans , Incidence , Models, Statistical , Nepal/epidemiology
13.
Parasit Vectors ; 12(1): 42, 2019 Jan 18.
Article in English | MEDLINE | ID: mdl-30658693

ABSTRACT

BACKGROUND: The expansion of dengue vectors from lowland plains to the upland hilly regions of Nepal suggests the likelihood of increased risk of dengue. Our objective was to assess the effects of meteorological variables on vector indices and populations of dengue vectors in two different ecological regions of Nepal. An entomological survey was conducted in Kathmandu and Lalitpur (upland) and Chitwan (lowland) of Nepal in three different seasons from July 2015 to May 2016. The effect of meteorological variables on vector indices (house index, container index and Breteau index) and Aedes spp. population abundance was analyzed. A gamma regression was used to fit the models for vector indices and a negative binomial regression was used to model Aedes spp. population abundance. RESULTS: Monsoon season showed higher values for vector indices and vector populations compared to post-monsoon and pre-monsoon. Overall, the factor temperature-rainfall effect had a more significant influence on vector indices compared to relative humidity. The regression models showed that relative humidity has a greater impact in Chitwan than in Kathmandu. Variation was observed in the effect of predictor variables on Aedes aegypti and Ae. albopictus abundance. CONCLUSIONS: Temperature and rainfall contribute to the vector indices in the upland hilly region while relative humidity contributes in the lowland plains. Since vector prevalence is not only linked to meteorological factors, other factors such as water storage practices, waste disposal, sanitary conditions and vector control strategy should also be considered. We recommend strengthening and scaling up dengue vector surveillance and control programmes for monsoon season in both upland and lowland regions in Nepal.


Subject(s)
Aedes/virology , Dengue Virus/physiology , Dengue/transmission , Mosquito Vectors/virology , Animals , Dengue/virology , Female , Humidity , Meteorological Concepts , Mosquito Control , Nepal/epidemiology , Population Dynamics , Prevalence , Rain , Risk Factors , Seasons , Temperature
14.
J Basic Microbiol ; 56(11): 1274-1288, 2016 Nov.
Article in English | MEDLINE | ID: mdl-27439917

ABSTRACT

Increasing evidence shows that nitric oxide (NO), a typical signaling molecule plays important role in development of plant and in bacteria-plant interaction. In the present study, we tested the effect of sodium nitroprusside (SNP)-a nitric oxide donor, on bacterial metabolism and its role in establishment of PGPR-plant interaction under salinity condition. In the present study, we adopted methods namely, biofilm formation assay, GC-MS analysis of bacterial volatiles, chemotaxis assay of root exudates (REs), measurement of electrolyte leakage and lipid peroxidation, and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for gene expression. GC-MS analysis revealed that three new volatile organic compounds (VOCs) were expressed after treatment with SNP. Two VOCs namely, 4-nitroguaiacol and quinoline were found to promote soybean seed germination under 100 mM NaCl stress. Chemotaxis assay revealed that SNP treatment, altered root exudates profiling (SS-RE), found more attracted to Pseudomonas simiae bacterial cells as compared to non-treated root exudates (S-RE) under salt stress. Expression of Peroxidase (POX), catalase (CAT), vegetative storage protein (VSP), and nitrite reductase (NR) genes were up-regulated in T6 treatment seedlings, whereas, high affinity K+ transporter (HKT1), lipoxygenase (LOX), polyphenol oxidase (PPO), and pyrroline-5-carboxylate synthase (P5CS) genes were down-regulated under salt stress. The findings suggest that NO improves the efficiency and establishment of PGPR strain in the plant environment during salt condition. This strategy may be applied on soybean plants to increase their growth during salinity stress.


Subject(s)
Gene Expression Regulation, Plant , Glycine max/genetics , Glycine max/microbiology , Nitric Oxide Donors/metabolism , Nitroprusside/metabolism , Pseudomonas/metabolism , Salt Tolerance , Soil Microbiology , Biofilms/growth & development , Catalase/genetics , Germination/drug effects , Lipid Peroxidation , Nitric Oxide/metabolism , Nitrite Reductases/genetics , Nitroprusside/pharmacology , Peroxidase/genetics , Plant Roots/microbiology , Quinolines/metabolism , Rhizosphere , Salt Tolerance/genetics , Seedlings/drug effects , Seedlings/growth & development , Seedlings/microbiology , Sodium Chloride/metabolism , Glycine max/drug effects , Glycine max/growth & development , Stress, Physiological , Volatile Organic Compounds/metabolism
15.
World J Microbiol Biotechnol ; 32(2): 19, 2016 Feb.
Article in English | MEDLINE | ID: mdl-26745979

ABSTRACT

At present, Artemisia annua L. is the major source of artemisinin production. To control the outbreaks of malaria, artemisinin combination therapies (ACTs) are recommended, and hence an ample amount of artemisinin is required for ACTs manufacture to save millions of lives. The low yield of this antimalarial drug in A. annua L. plants (0.01-1.1%) ensues its short supply and high cost, thus making it a topic of scrutiny worldwide. In this study, the effects of root endophyte, Piriformospora indica strain DSM 11827 and nitrogen fixing bacterium, Azotobacter chroococcum strain W-5, either singly and/or in combination for artemisinin production in A. annua L. plants have been studied under poly house conditions. The plant growth was monitored by measuring parameters like height of plant, total dry weight and leaf yield with an increase of 63.51, 52.61 and 79.70% respectively, for treatment with dual biological consortium, as compared to that of control plants. This significant improvement in biomass was associated with higher total chlorophyll content (59.29%) and enhanced nutrition (especially nitrogen and phosphorus, 55.75 and 86.21% respectively). The concentration of artemisinin along with expression patterns of artemisinin biosynthesis genes were appreciably higher in dual treatment, which showed positive correlation. The study suggested the potential use of the consortium P. indica strain DSM 11827 and A. chroococcum strain W-5 in A. annua L. plants for increased overall productivity and sustainable agriculture.


Subject(s)
Artemisia annua/metabolism , Artemisia annua/microbiology , Artemisinins/metabolism , Azotobacter/metabolism , Basidiomycota/metabolism , Artemisia annua/genetics , Biomass , Biosynthetic Pathways , Chlorophyll/metabolism , Nitrogen/chemistry , Nitrogen/metabolism , Nitrogen Fixation , Phosphorus/chemistry , Phosphorus/metabolism , RNA/isolation & purification , Real-Time Polymerase Chain Reaction , Symbiosis
16.
World J Microbiol Biotechnol ; 32(1): 4, 2016 Jan.
Article in English | MEDLINE | ID: mdl-26712619

ABSTRACT

The present study focused on the overproducing mutant of a plant growth promoting rhizobacterium (PGPR) Pseudomonas simiae strain AU (MTCC-12057) for significant drought tolerance in mung bean plants. Five mutants namely AU-M1, AU-M2, AU-M3, AU-M4 and AU-M5 were made after treatment of wild type strain with N-methyl-N-nitro-N-nitrosoguanidine. Mutant strain AU-M4 was recorded for enhanced ACC deaminase (ACC-D) activity, indole acetic acid (IAA) production and inorganic phosphate (Pi) solubilization compared to wild strain and other four mutant strains under drought condition. AU-M4 showed higher phosphate solubilization index (8.17) together with higher ACC-D activity (98 nmol/mg/h) and IAA concentration (69.35 µg/ml) compared with the wild type P. simiae strain AU ACC-D activity (79 nmol/mg/h) and IAA concentration (38.98 µg/ml) respectively. In this report, we investigated the effect of both wild and mutant type bacterial strain on mung bean plants under drought stress. Results showed that mutant AU-M4 and wild type strain AU inoculated plants exhibited superior tolerance against drought stress, as shown by their enhanced plant biomass (fresh weight), higher water content, higher proline accumulation and lower osmotic stress injury. Mutant AU-M4 and wild strain AU inoculated plants reduced the ethylene level by 59 and 45% respectively, compared to the control under stress condition. Furthermore, bacterial inoculated plants showed enhanced induced systemic drought tolerance by reducing stomata size and net photosynthesis resulting higher water content in mung bean plants that may help in survival of plants during drought condition. To mitigate the effects of drought stress, use of PGPR will be needed to ensure sufficient production of food from crop plants. Taking current leads available, concerted future research is needed in this area, particularly on field evaluation with application of potential microorganisms.


Subject(s)
Droughts , Fabaceae/microbiology , Fabaceae/physiology , Pseudomonas/physiology , Biomass , Carbohydrate Metabolism , Carbon-Carbon Lyases/metabolism , Ethylenes/metabolism , Fabaceae/growth & development , Indoleacetic Acids/metabolism , Mutation , Osmotic Pressure , Proline/metabolism , Pseudomonas/genetics , Pseudomonas/growth & development , Pseudomonas/metabolism , Rhizobium/growth & development , Soil Microbiology
17.
Arch Microbiol ; 197(7): 869-81, 2015 Sep.
Article in English | MEDLINE | ID: mdl-26123239

ABSTRACT

It is our consensus that plants survive and flourish in stressed ecosystems because of endosymbiotic organisms that have co-evolved and were essential for their adaptation to changing environments. Some of these microbial components are noncultivable and vertically transmitted from generation to generation. They represent a vast reservoir of heritable DNA that can enhance plant performance in changing environments and add genetic flexibility to adaptation of long-lived plants. If such endophytes can be identified that not only persist in progeny of novel hosts, but can confer benefits in mechanized, agricultural systems, they would be increasingly important in agricultural production and lead to a rapid and economical method of providing novel germplasms of native and crop plants. In the present review, authors advocate the deployment of fungal diversity and its role to overcome the biotic stress in plants. Endophytic fungal association with plants helps it to protect from various pathogen and pests and adapt to survive in harsh biotic and abiotic stress condition.


Subject(s)
Endophytes/physiology , Fungi/physiology , Plants/microbiology , Stress, Physiological/physiology , Adaptation, Physiological , Ecosystem , Symbiosis
18.
Mol Biol Rep ; 41(11): 7631-8, 2014 Nov.
Article in English | MEDLINE | ID: mdl-25086625

ABSTRACT

Plant viruses encode suppressors of posttranscriptional gene silencing, an adaptive antiviral defense responses that confines virus infection. Previously, we identified single-stranded DNA satellite (also known as DNA-ß) of ~1,350 nucleotides in length associated with Croton yellow vein mosaic begomovirus (CYVMV) in croton plants. The expression of genes from DNA-ß requires the begomovirus for packaged, replication, insect transmission and movement in plants. The present study demonstrates the effect of the ßC1 gene on the silencing pathway as analysed by using both transgenic systems and transient Agrobacterium tumefaciens based delivery. Plants that carry an intron-hairpin construct covering the ßC1 gene accumulated cognate small-interfering RNAs and remained symptom-free after exposure to CYVMV and its satellite. These results suggest that ßC1 interferes with silencing mechanism.


Subject(s)
Begomovirus/genetics , Croton/genetics , Croton/immunology , Croton/virology , DNA, Satellite/genetics , RNA Interference/immunology , Agrobacterium tumefaciens , Begomovirus/immunology , Blotting, Northern , Blotting, Southern , DNA Primers/genetics , Plasmids/genetics , Polymerase Chain Reaction , RNA, Small Interfering/genetics , Transformation, Genetic
19.
Planta ; 239(5): 1027-40, 2014 May.
Article in English | MEDLINE | ID: mdl-24504695

ABSTRACT

The aim of the present study was to analyze induced expression of defense-related proteins in the soybean plants by rhizobacterial stain Carnobacterium sp. SJ-5 upon challenge inoculation with Fusarium oxysporum. Determination of the enzymatic activity of the different defense-related enzymes, phenylalanine ammonia lyase (PAL), lipoxygenase (LOX), peroxidase (POD) and polyphenol oxidase (PPO) was performed in the major parts of Glycine max L. Merrill using spectrophotometric method. Native-polyacrylamide gel electrophoresis analysis of the POD and PPO was employed followed by activity staining to find out the isoforms of respective enzymes. Activities of the PAL, LOX, POD and PPO were found to be highest in the bacterized root tissue of the soybean plants challenged with F. oxysporum. Isoform analysis revealed that PPO1, PPO4 and POD2 isoforms were expressed at higher levels in bacterized soybean root tissues challenge inoculated with the pathogen. Conclusively it was found that bacterial strain Carnobacterium sp. SJ-5 protect soybean plants from wilt disease caused by F. oxysporum by elicitation of the defense-related enzymes.


Subject(s)
Carnobacterium/physiology , Fusarium/physiology , Glycine max/immunology , Glycine max/microbiology , Plant Diseases/immunology , Plant Diseases/microbiology , Plant Proteins/metabolism , Antifungal Agents/pharmacology , Biological Assay , Carnobacterium/drug effects , Catechol Oxidase/metabolism , Crosses, Genetic , Fusarium/drug effects , Germination/drug effects , Hydrogen-Ion Concentration , Isoenzymes/metabolism , Lipoxygenase/metabolism , Microbial Sensitivity Tests , Native Polyacrylamide Gel Electrophoresis , Peroxidase/metabolism , Phenylalanine Ammonia-Lyase/metabolism , Phosphates/metabolism , Seeds/drug effects , Seeds/metabolism , Seeds/microbiology , Siderophores/metabolism , Solubility , Glycine max/growth & development , Glycine max/metabolism
20.
World J Microbiol Biotechnol ; 30(2): 399-406, 2014 Feb.
Article in English | MEDLINE | ID: mdl-23933805

ABSTRACT

Wilt disease of soybean caused by a very common soil-borne fungus, Fusarium oxysporum is one of the most destructive diseases of the crop. The aim of the present study was to characterize plant growth-promotion activities and induced resistance of a rhizobacterial strain for the soybean plant against F. oxysporum. Rhizobacterium strain SJ-5 exhibited plant growth-promotion characteristics and antagonistic activity against the test pathogen on dual plate assay. It was identified as a Carnobacterium sp. A 950 bp PCR product was amplified from Carnobacterium sp. strain SJ-5, using zwittermicin A self-resistance gene-specific primers (zmaR). The strain produced indole 3-acetic acid (19 µg/ml) in the presence of salt stress and exhibited growth in Dworkin and Foster salt medium amended with 1-aminocyclopropane-1-carboxylate (ACC) through ACC deaminase activity (277 nmol/mg/h) as compared to the control. Strain seeds treated with the strain significantly enhanced the quorum of healthy plants after challenge inoculation at 14 days after seeding. An increase in the activity of stress enzymes after challenge inoculation with the test pathogen is reported. Treatment with the bacterium resulted in an increase in the chlorophyll content in the leaves in comparison with challenge-inoculated plants.


Subject(s)
Carnobacterium/physiology , Fusarium/growth & development , Glycine max/growth & development , Glycine max/microbiology , Plant Diseases/prevention & control , Carnobacterium/genetics , Carnobacterium/metabolism , Chlorophyll/analysis , Heat-Shock Proteins/biosynthesis , Indoleacetic Acids/metabolism , Plant Growth Regulators/metabolism , Plant Leaves/chemistry , Glycine max/enzymology
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