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1.
Front Psychol ; 13: 881019, 2022.
Article in English | MEDLINE | ID: mdl-35846680

ABSTRACT

The purpose of this research is to investigate the effectiveness of Digital Content Marketing (DCM) on a Mixed Reality (MR) training platform environment with the consideration of online purchase intention (OPI) through social media. E-commerce today encounters several common issues that cause customers to have reservations to purchase online. With the absence of physical contact points, customers often perceive more risks when making purchase decisions. Furthermore, online retailers often find it hard to engage customers and develop long-term relationships. In this research, a Structural Equation Model (SEM) is proposed to examine the efficacy of DCM from both immediate and long-term OPI. The results examine whether adopting DCM on an MR training platform environment through social media brings positive results in OPI. Empirical research was carried out through online questionnaires collected in 2021 and 2022. A total of 374 questionnaires were qualified for data analysis in this study, conducted with IBM SPSS and AMOS. The results imply that DCM is critical to stimulating both immediate and long-term OPI. The immediate OPI is positively affected by increasing perceived value through MR in DCM. Regarding the long-term OPI, increased customer engagement with DCM under MR environment can cultivate brand trust and significantly affect the long-term OPI.

2.
Opt Express ; 25(8): 9088-9093, 2017 Apr 17.
Article in English | MEDLINE | ID: mdl-28437983

ABSTRACT

A fast and non-iterative method for generating a phase-only hologram, hereafter referred to as the patterned-phase-only hologram (PPOH), is reported in this paper. Briefly, a phase mask with a periodic phase pattern is added to the source image, and converted into a hologram. Subsequently, only the phase component is retained as a phase-only hologram. Experimental evaluation reveals that the visual quality of the reconstructed images of the PPOH generated with our proposed method is favorable, and superior to that obtained with existing methods.

3.
Opt Express ; 24(20): 23390-23395, 2016 Oct 03.
Article in English | MEDLINE | ID: mdl-27828405

ABSTRACT

If an image is uniformly down-sampled into a sparse form and converted into a hologram, the phase component alone will be adequate to reconstruct the image. However, the appearance of the reconstructed image is degraded with numerous empty holes. In this paper, we present a low complexity and non-iterative solution to this problem. Briefly, two phase-only holograms are generated for an image, each based on a different down-sampling lattice. Subsequently, the holograms are displayed alternately at high frame rate. The reconstructed images of the 2 holograms will appear to be a single, densely sampled image with enhance visual quality.

4.
Opt Express ; 22(21): 25208-14, 2014 Oct 20.
Article in English | MEDLINE | ID: mdl-25401554

ABSTRACT

We present a novel non-iterative method for generating phase-only Fresnel holograms. The intensity image of the source object scene is first down-sampled with uniform grid-cross lattices. A Fresnel hologram is then generated from the intensity and the depth information of the sampled object points. Subsequently, only the phase component of the hologram is preserved, resulting in a pure phase hologram that we call the sampled-phase-only hologram (SPOH). Experimental evaluation reveals that the numerical, as well as the optical reconstructed images of the proposed phase-only hologram derived with our method are of high visual quality. Moreover, the reconstructed optical image is brighter, and less affected by phase noise contamination on the hologram as compared with those generated with existing error-diffusion approaches.


Subject(s)
Algorithms , Holography/methods , Humans , Image Processing, Computer-Assisted , Numerical Analysis, Computer-Assisted
5.
J Biol Chem ; 274(14): 9455-62, 1999 Apr 02.
Article in English | MEDLINE | ID: mdl-10092627

ABSTRACT

The Prp19p protein of the budding yeast Saccharomyces cerevisiae is an essential splicing factor and is associated with the spliceosome during the splicing reaction. We have previously shown that Prp19p is not tightly associated with small nuclear ribonucleoprotein particles but is associated with a protein complex consisting of at least eight protein components. By sequencing components of the affinity-purified complex, we have identified Cef1p as a component of the Prp19p-associated complex, Ntc85p. Cef1p could directly interact with Prp19p and was required for pre-mRNA splicing both in vivo and in vitro. The c-Myb DNA binding motif at the amino terminus of Cef1p was required for cellular growth but not for interaction of Cef1p with Prp19p or Cef1p self-interaction. We have identified a small region of 30 amino acid residues near the carboxyl terminus required for both cell viability and protein-protein interactions. Cef1p was associated with the spliceosome in the same manner as Prp19p, i.e. concomitant with or immediately after dissociation of U4. The anti-Cef1p antibody inhibited binding to the spliceosome of Cef1p, Prp19p, and at least three other components of the Prp19p-associated complex, suggesting that the Prp19p-associated complex is likely associated with the spliceosome and functions as an integral complex.


Subject(s)
Cell Cycle Proteins/metabolism , Fungal Proteins/metabolism , RNA Precursors/metabolism , RNA Splicing , Saccharomyces cerevisiae Proteins , Transcription Factors/metabolism , Amino Acid Sequence , Electrophoresis, Polyacrylamide Gel , Macromolecular Substances , Molecular Sequence Data , RNA Splicing Factors , RNA-Binding Proteins , Saccharomyces cerevisiae , Spliceosomes/metabolism , Structure-Activity Relationship
6.
Opt Lett ; 22(8): 510-2, 1997 Apr 15.
Article in English | MEDLINE | ID: mdl-18183250

ABSTRACT

An efficient nondegenerate four-wave-mixing process in a highly birefringent optical fiber pumped by a dye laser is reported. The output beam from a dye laser (at ~610 nm) was found to mix with the accompanying superfluorescent light (from 575 to 600 nm) in a birefringent fiber to generate a distinct frequency-shifted beam, which could be Stokes or anti-Stokes radiation, depending on which mode of the fiber was excited. The frequency shift was comparable with that observed in the well-known pump-divided degenerate process, as confirmed by the theory.

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