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1.
Arch Physiol Biochem ; 118(3): 121-7, 2012 Jul.
Article in English | MEDLINE | ID: mdl-22540353

ABSTRACT

The adipocyte enlargement is associated with an increase in the cytoplasmic lipid content, but how the plasma membrane area follows this increase is poorly understood. We monitored single-cell membrane surface area fluctuations, which mirror the dynamics of exocytosis and endocytosis. We employed the patch-clamp technique to measure membrane capacitance (C(m)), a parameter linearly related to the plasma membrane area. Specifically, we studied whether insulin affects membrane area dynamics in adipocytes. A five-minute cell exposure to insulin increased resting C(m) by 12 ± 4%; in controls the change in C(m) was not different from zero. We measured cell diameter of isolated rat adipocytes microscopically. Twenty-four hour exposure of cells to insulin resulted in a significant increase in cell diameter by 5.1 ± 0.6%. We conclude that insulin induces membrane area increase, which may in chronic hyperinsulinemia promote the enlargement of plasma membrane area, acting in concert with other insulin-mediated metabolic effects on adipocytes.


Subject(s)
Adipocytes/drug effects , Cell Enlargement/drug effects , Cell Membrane/drug effects , Insulin/pharmacology , Adipocytes/cytology , Adipocytes/physiology , Animals , Cell Size , Cells, Cultured , Electric Capacitance , Endocytosis/physiology , Exocytosis/physiology , Male , Membrane Potentials/drug effects , Membrane Potentials/physiology , Patch-Clamp Techniques , Rats , Rats, Wistar , Single-Cell Analysis
2.
Pflugers Arch ; 439(3 Suppl): R148-9, 2000.
Article in English | MEDLINE | ID: mdl-10653173

ABSTRACT

We monitored secretory activity of single rat melanotrophs by the patch-clamp membrane capacitance measurements (Cm). Secretory activity was stimulated by cytosol dialysis with a patch-pipette solution containing 1 microM [Ca2+]i. Actin cytoskeleton was disaggregated by pretreating cells with Clostridium spiroforme toxin, which specifically ADP-ribosylates cellular actin. The extent of cytoskeleton disaggregation was monitored by phalloidin immunostaining. The maximal rate of secretion increases two folds in toxin-treated cells in comparison to controls, whereas the extent of calcium-induced secretory response was similar to that obtained in the non-treated cells. The results show that the subcortical actin network attenuates the rate of secretory activity, which we interpret to reflect a barrier function of cytoskeleton for exocytosis.


Subject(s)
Actins/metabolism , Cytoskeleton/metabolism , Exocytosis/physiology , Melanocyte-Stimulating Hormones/metabolism , Pituitary Gland/metabolism , Animals , Bacterial Toxins/pharmacology , Calcium/pharmacology , Cytoskeleton/drug effects , Male , Patch-Clamp Techniques , Pituitary Gland/cytology , Pituitary Gland/drug effects , Pituitary Gland/physiology , Rats , Rats, Wistar
3.
J Physiol ; 521 Pt 2: 389-95, 1999 Dec 01.
Article in English | MEDLINE | ID: mdl-10581310

ABSTRACT

1. We measured membrane capacitance (Cm) in cultured rat melanotrophs pretreated with Clostridium spiroforme toxin (CST), which specifically depolymerizes cortical filamentous actin (F-actin). Phalloidin staining confirmed that CST treatment depolymerised the F-actin. 2. In control cells, cytosol dialysis with 1 microM Ca2+i increased Cm by 23 +/- 4 % (n = 11) relative to the resting Cm 400 s after the start of patch rupture. In CST-treated cells the increase in Cm was 32 +/- 5 % (n = 15), not significantly different from controls. The rate of Cm increase was affected transiently by CST treatment, peaking at 1 min after patch rupture. The maximal rate of Cm increase was 4.27 +/- 0.85 fF s-1 (n = 12; measured 200 s after the start of patch rupture) in controls and 8.0 +/- 1.35 fF s-1 (n = 23; measured 75 s after the start of patch rupture) in CST-treated cells (P < 0.01). 3. In control cells cytosol dialysis with 0 microM Ca2+i decreased Cm by 9 +/- 3 % (n = 7), in CST-treated cells Cm increased by 11 +/- 3 % (n = 7) relative to resting Cm 400 s after the start of cytosol dialysis. The rate of change in Cm remained constant (controls: -1 to -2 fF s-1; CST treatment: 1-2 fF s-1). 4. Transient and sustained effects of CST treatment on changes in Cm at high or low [Ca2+]i, respectively, suggest a distinct role of cytoskeleton in Ca2+-dependent and Ca2+-independent changes in Cm. Transient enhancement of the rate of Cm by CST is consistent with a barrier role of cytoskeleton in regulated exocytosis. The sustained effect of CST on Ca2+-independent changes in Cm suggests cytoskeletal involvement in endocytosis.


Subject(s)
ADP Ribose Transferases , Actins/metabolism , Bacterial Toxins/pharmacology , Cytoskeleton/metabolism , Melanocytes/metabolism , Actins/drug effects , Animals , Calcium/pharmacology , Cytoskeleton/drug effects , Electric Conductivity , Exocytosis/drug effects , Exocytosis/physiology , Male , Melanocytes/drug effects , Microscopy, Fluorescence , Patch-Clamp Techniques , Phalloidine , Polymers/metabolism , Rats , Rats, Wistar
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