ABSTRACT
Hyperthermia (37 degrees C permanently) and heat shock (42 degrees C for 10 min, and then 27 degrees C) retarded the elimination of chloroplasts from the flagellate Euglena gracilis induced by quinolone antibacterial chemotherapeutics (OA, NA, Cnx, Ofx, Cpfx, Enx, Nfx) in comparison with their action at 27 degrees C. In the case of OA, NA, and Cnx those hyperthermic conditions completely blocked their action against chloroplasts. On the other hand, both temperature regimes accelerated the antichloroplast activity of the mutagens/carcinogens nitrosoguanidine and furylfuramide.
Subject(s)
Anti-Bacterial Agents/toxicity , Chloroplasts/drug effects , Hot Temperature/adverse effects , Mutagens/pharmacology , Quinolines/toxicity , Animals , Euglena gracilis , Furylfuramide/toxicity , Nitrosoguanidines/toxicityABSTRACT
The effect of heat-shock treatment (42 degrees C for 15 min) on the ability of four mutagens and carcinogens to induce hereditary bleaching in Euglena gracilis cells was investigated. All four mutagens (treatment time: 1-24 h) tested after heat shock increased the frequency of bleached mutants of Euglena gracilis.
Subject(s)
Carcinogens/pharmacology , Euglena gracilis/drug effects , Hot Temperature , Mutagens/pharmacology , Mutation , Animals , Euglena gracilis/genetics , Euglena gracilis/ultrastructure , Microscopy, Electron , Mutagenicity TestsABSTRACT
Mutagenicity of three selected series of 2-furylethylene was determined by the Ames test. These included: nine alkylesters and eleven N-alkylamides of 5-nitro-2-furylacrylic acid (NFAA) and ten derivatives differing not only at exocyclic double bond, but also in the position 5 of the furan ring. Mutagenicity of the derivatives depends on the presence of the 5-nitro-furan centre in the molecule; side chains in the position 2 modify the degree of mutagenicity. Among the derivatives of NFAA tested as changing the substituents virtually does not affect chemical properties of the 5-nitrofuran ring. Mutagenicity of the n-alkyl congeners decreases linearly with increasing lipophilicity. Mutagenicity of the derivatives with branched alkyl substituents is lower than expected from the behaviour of the n-alkyl homologues.
