Public opinions on seven different stray cat population management scenarios in Flanders, Belgium.

Stray cat population management is an important worldwide issue. Understanding citizen attitudes towards stray cat control options is vital to the success of controlling stray cat numbers, as public perception affects the acceptance of, support for and collaboration in stray cat management policies. Audience segmentation, as to enable each group to be engaged in the stray cat management policy, is important for the success of the interventions. Therefore a web-based survey was conducted among Flemish citizens in order to examine differences in acceptance towards seven management scenarios: household cat neutering with financial support for the owner, household cat neutering without financial support for the owner, encouraging responsible household cat ownership, trapping stray cats and taking them to a shelter, trapping and neutering stray cats for release into a managed "cat colony" (composed by so called "community cats"), trapping and killing of stray cats, and undertaking no action. A total of 4059 valid responses were collected and the proportions of agreement were compared across the different management scenarios using the two-sample z-test. Interactions among factors that influenced each management scenario were investigated using the CHAID (Chi-squared Automatic Interaction Detection) analysis and visualized on a tree. Our results showed that fostering responsible household cat ownership (89.9%) and conversion of stray cats to "community cats" (76.3%) were most supported by respondents in our sample (which consisted mainly of females, cat-lovers, and families without children). Least supported were the killing of stray cats (7.7%) and undertaking no action (3.3%). The demographic analyses revealed that for the acceptance of management scenarios there were three important factors (attitude towards cats, area of residence, and gender), two weaker factors (education and having children) and two which had almost no impact (age and cat ownership). We propose that future studies should focus on the effect of 'area of residence', 'having children' and 'education'. In conclusion, our research confirms that management of and communication on stray cat strategies should not be developed with a one-size-fits-all approach. Efforts should be tailored to each audience segment, thus adapted to the area of residence and human characteristics.

Killing individual poultry on-farm-a survey among veterinarians and farmers.

To date, information about reasons to select and kill poultry on-farm and which method veterinarians and poultry producers preferably use is scarce. Little is also known about their knowledge of the legislation regarding on-farm killing methods and of methods alternative to the one(s) they use, as well as their perception of those alternatives. In this study, Flemish poultry veterinarians (n = 13), broiler chicken producers (n = 27), and turkey producers (n = 4) were surveyed on killing methods they currently use in practice and alternative methods, on their opinion about what constitutes an appropriate method for on-farm killing of poultry, and on their reasons for killing. All poultry veterinarians and chicken producers who filled out the survey kill poultry by manual cervical dislocation (CD), whereas some turkey farmers also indicated killing by percussive blow to the head (n = 1) or exsanguination (n = 1). Turkey producers seem to be more inclined not to kill animals with injuries or symptoms of disease as compared to veterinarians or chicken producers, such that moribund turkeys are more likely to remain in the flock. Veterinarians considered the following properties of a killing method important: animal friendliness, applicability inside the stable, a high success rate, and time efficiency. Producers ranked the properties similarly, but for them, ease of performance and cost-efficiency were more important than applicability inside the stable. Producers scored those killing properties rather positively for manual as well as mechanical CD. Veterinarians and chicken producers considered the captive bolt method to be easy to perform, to have a high success rate, to be feasible to perform in the stable, and to be animal-friendly. Turkey producers, however, had doubts about the latter 2 properties. Gas, injection, and electrocution were inferior methods to kill poultry according to producers. In conclusion, manual CD is the most common method for killing broiler chickens and turkeys, and knowledge of, and experience with, alternative methods is very limited, both among veterinarians and producers. Informing them about legislation and training for the use of alternative killing techniques are recommended.

Physiological stress reactivity and recovery related to behavioral traits in dogs (Canis familiaris).

This study investigated whether stress responsiveness (in one context) can be used to predict dog behavior in daily life. On two occasions (NT1 = 32 puppies; NT2 = 16 young adults), dogs' physiological stress response after a behavioral test at home was measured in terms of reactivity (10 min post-test) and recovery (40 min post-test) for three salivary markers: cortisol, chromogranin A (CgA) and secretory immunoglobulin A (sIgA). For each marker, it was determined whether dogs with a strong physiological response displayed different behavior in daily life compared to dogs with a weaker physiological response. The results revealed three main findings: first, for CgA and cortisol, different patterns were identified according to sample time. High reactivity related to desirable traits, whereas slow recovery after the behavioral test related to undesirable traits. The findings suggest that increased levels of CgA and cortisol 10 minutes after the behavioral test reflected an adaptive stress response, whereas elevated levels 40 minutes after the test reflected unsuccessful coping. Second, patterns for sIgA differed from CgA and cortisol: significant associations were only found with behavioral traits at T2, mostly considered desirable and related to Trainability. Possibly, the delayed reaction pattern of sIgA caused this difference between markers, as sIgA reflects the (secondary) immune response to stress, due to immunosuppressive effects of cortisol. Third, predictive capacity of puppies' physiological stress response (T1) was inconclusive, and contrary relations were found with behavioral traits at T2, suggesting that developmental factors play an important role. This study provides new insights about the relation between stress physiology and behavioral traits, and methodological advice is given to study these patterns further. In conclusion, physiological markers could provide additional insights in dogs' tendencies to display certain behaviors, especially at the young adult stage. Further studies are needed to confirm these patterns.

Rejections in an non-purpose bred assistance dog population: Reasons, consequences and methods for screening.

Assistance dogs aid people with various impairments on a daily basis. To become an assistance dog, a strict selection procedure and intensive training period must be successfully completed. Consequently, not every dog acquired for this purpose, becomes an assistance dog. The purpose of this study was to investigate reasons for failure and the financial consequences thereof for assistance dog associations that do not have a dedicated breeding program for their dogs. Data were collected for a total of 537 dogs enlisted between 2001 and 2015 and purchased out of the general dog population by five Belgian assistance dog associations. Only 60 percent of the dogs actually became an assistance dog and the main reasons for failure were related to undesirable behavioural characteristics and orthopaedic disorders. The estimated average financial loss per rejected dog was found to be 10524 euro. A detailed comparison of the two most popular breeds (Golden Retriever and Labrador Retriever) within the guide dogs and mobility assistance dogs revealed no significant difference in probability of successfully completing the training. However, a comparison of orthopaedic screening methods revealed a higher rejection with computed tomography for elbow dysplasia and laxity-based radiographical techniques for hip dysplasia compared to radiography and the standard ventrodorsal hip extend radiograph alone, respectively. Based on these results, we provide several suggestions to increase the probability of success.

On the origin of puppies: breeding and selling procedures relevant for canine behavioural development.

The success of the dog as a companion animal has undeniably led to a shift in dog breeding practices. While effects of inbreeding or large-scale breeding have given rise to numerous studies about potentially related health issues, it remains unclear to what extent behavioural development of dogs is influenced. By investigating the environment of puppies while at the breeder, the authors aimed to make an inventory of current practices regarding management, socialisation and environmental learning and subsequently to identify potential differences between breeder types. The cross-sectional study, conducted during 2016, revealed considerable variability in environment among dog breeders. Small-scale breeders, and especially occasional breeders (less than 10 adult dogs on-site) provided most enrichment, both social and non-social, by, for instance, providing more outdoor access for pregnant dams and puppies or by providing access to visitors more freely. Environmental stimuli were less controlled in occasional breeders, raising the debate about quantity versus quality of stimuli at a young age. Large-scale breeders declared to screen potential owners less intensely and time to advise them was limited. To the authors' knowledge, this is the first study that compares a large number of environmental factors between the different dog breeding categories.

Faculty's work engagement in patient care: impact on job crafting of the teacher tasks.

BACKGROUND: High levels of work engagement protect against burnout. This can be supported through the work environment and by faculty themselves when they try to improve their work environment. As a result, they can become more engaged and better performers. We studied the relationship between adaptations by physicians to improve their teaching work environment, known as job crafting, and their energy levels, or work engagement, in their work as care provider and teacher. Job crafting encompasses seeking social (i) and structural (ii) resources and challenges (iii) and avoiding hindrances (iv). METHODS: We established a cross-sectional questionnaire survey in a cohort of physicians participating in classroom and clinical teaching. Job crafting and work engagement were measured separately for physicians' clinical and teaching activities. We analyzed our data using structural equation modelling controlling for age, gender, perceived levels of autonomy and participation in decision making. RESULTS: 383 physicians were included. Physicians' work engagement for patient care was negatively associated with two job crafting behaviors in the teaching roles: seeking structural resources (classroom teaching: ß = - 0.220 [95% CI: -0.319 to - 0.129]; clinical teaching: ß = - 0.148 [95% CI: -0.255 to - 0.042]); seeking challenges (classroom teaching: ß = - 0.215 [95% CI: -0.317 to - 0.113]; clinical teaching:, ß = - 0.190 [95% CI: -0.319 to - 0.061]). Seeking social resources and avoiding hindrances were unaffected by physicians' work engagement for patient care. CONCLUSIONS: High engagement for teaching leads to job crafting in teaching. High engagement for patient care does not lead to job crafting in teaching.

Heat stress in cows at pasture and benefit of shade in a temperate climate region.

Under temperate climates, cattle are often at pasture in summer and are not necessarily provided with shade. We aimed at evaluating in a temperate region (Belgium) to what extent cattle may suffer from heat stress (measured through body temperature, respiration rate and panting score, cortisol or its metabolites in milk, and feces on hot days) and at assessing the potential benefits of shade. During the summer of 2012, 20 cows were kept on pasture without access to shade. During the summer of 2011, ten cows had access to shade (young trees with shade cloth hung between them), whereas ten cows had no access. Climatic conditions were quantified by the Heat Load Index (HLI). In animals without access to shade respiration rates, panting scores, rectal temperatures, and milk cortisol concentrations increased as HLI increased in both 2011 and 2012. Fecal cortisol metabolites varied with HLI in 2011 only. When cattle had access to shade, their use of shade increased as the HLI increased. This effect was more pronounced during the last part of the summer, possibly due to better acquaintance with the shade construction. In this case, shade use increased to 65% at the highest HLI (79). Shade tempered the effects on respiration, rectal temperature, and fecal cortisol metabolites. Milk cortisol was not influenced by HLI for cows using shade for > 10% of the day. Therefore, even in temperate areas, cattle may suffer from heat when they are at pasture in summer and providing shade can reduce such stress.

Is being a medical educator a lonely business? The essence of social support.

CONTEXT: Social support helps prevent burnout and promotes its positive opposite, work engagement. With higher work engagement performance increases. The context-specific aspects of social support for medical educators, in their educator role, are unknown. To help facilitate social support our study describes the essential elements of social support and their meaning for medical educators. METHODS: We held interviews with medical educators purposefully sampled for diverse backgrounds and working circumstances and who spent a considerable amount of time on education. Both clinicians and basic scientists participated. The Pictor technique guided the interviews. Participants were invited to talk about the breadth of social support and elaborate on meaningful experiences. Template analysis was used for a descriptive phenomenological approach. RESULTS: Thirteen medical educators were interviewed. We identified four themes: (i) sources of support and their intent (e.g. a superior with the intent to stimulate personal growth); (ii) the materialisation of support (e.g. sought or offered); (iii) its manifestation (e.g. the act of providing protected time); and (iv) the overarching effect of social support, both in terms of practical effects and the meaning of support. We identified three sorts of meanings of social support for educators. Receiving support could lead to (i) feeling reassured and confident; (ii) feeling encouraged and determined and (iii) a sense of relatedness and acknowledgement of the educator role. CONCLUSION: Support for education comes from a wide range of sources because it is both sought and offered beyond the boundaries of the educational role. The resulting differences in support provided necessitate that educational leaders and policymakers consider the sources available to each educator, connecting educators where necessary. When facilitating or designing social support it is important that the need to feel reassured, encouraged or related is met.

Prepubertal gonadectomy in cats: different injectable anaesthetic combinations and comparison with gonadectomy at traditional age.

Anaesthetic and analgesic effects of three different injectable anaesthetic combinations for prepubertal gonadectomy (PPG) in cats were studied. One anaesthetic protocol was compared with a similar one for gonadectomy at traditional age (TAG). Kittens were randomly assigned to PPG or TAG. For PPG, three different protocols were compared: (1) intramuscular (IM) administration of 60 µg/kg dexmedetomidine plus 20 µg/kg buprenorphine followed by an IM injection of the anaesthetic agent (20 mg/kg ketamine) (DB-IM protocol); (2) oral transmucosal (OTM) administration of 80 µg/kg dexmedetomidine plus 20 µg/kg buprenorphine followed by an IM injection of 20 mg/kg ketamine combined with 20 µg/kg dexmedetomidine (DB-OTM protocol); (3) IM injection of a 40 µg/kg medetomidine-20 µg/kg buprenorphine-20 mg/kg ketamine combination (MBK-IM protocol). For TAG, a DB-IM protocol was used, but with different doses for dexmedetomidine (40 µg/kg) and ketamine (5 mg/kg). All cats (PPG and TAG) received a non-steroidal anti-inflammatory before surgery. Anaesthetic and analgesic effects were assessed pre- and postoperatively (until 6 h). Cumulative logit, linear and logistic regression models were used for statistical analysis. Compared with the DB-OTM protocol, the DB-IM and MBK-IM protocols provided better anaesthesia with fewer adverse effects in PPG cats. Postoperative pain was not significantly different between anaesthetic protocols. PPG and TAG cats anaesthetised with the two DB-IM protocols differed significantly only for sedation and pain scores, but sedation and pain scores were generally low. Although there were no anaesthesia-related mortalities in the present study and all anaesthetic protocols for PPG in cats provided a surgical plane of anaesthesia and analgesia up to 6 h postoperatively, our findings were in favour of the intramuscular (DB-IM and MBK-IM) protocols.

The effect of different working definitions on behavioral research involving stereotypies in Mongolian gerbils (Meriones unguiculatus).

Many sources of variation in animal experiments are related to characteristics of the animal or its husbandry conditions. In ethologic studies, observational methods can also affect interexperimental variation. Different descriptions for a behavior can lead to divergent findings that may be incorrectly attributed to other factors if not recognized as stemming from a classification dissonance. Here we discuss 2 observational studies in Mongolian gerbils (Meriones unguiculatus). The first study describes how data vary when 2 different working definitions are used for stereotypic digging: WD(mor), a definition based on a morphologic description of the behavior, and WD(12), a definition that relies mainly on a duration criterion of digging bouts (greater than 12 s). The total duration and number of stereotypic bouts were 22.0% and 63.1% lower, respectively, when WD(12) was applied compared with WD(mor). However, strong correlations existed between data generated by WD(mor) and WD(12), indicating that the 2 definitions yielded qualitatively similar results. The second study provides the first report that laboratory gerbils develop stereotypic behavior that is characterized by alternating bouts of digging and bar-gnawing. Of the 1685 stereotypy bouts investigated, 9.1% comprised both stereotypies, 87.6% consisted of digging only, and 3.3% consisted of bar gnawing only. Working definitions that neglect combined stereotypies can result in considerable underestimation of stereotypic behavior in Mongolian gerbils.

The contribution of each anterior cruciate ligament bundle to the Lachman test: a cadaver investigation.

The clinical diagnosis of a partial tear of the anterior cruciate ligament (ACL) is still subject to debate. Little is known about the contribution of each ACL bundle during the Lachman test. We investigated this using six fresh-frozen cadaveric lower limbs. Screws were placed in the femora and tibiae as fixed landmarks for digitisation of the bone positions. The femur was secured horizontally in a clamp. A metal hook was screwed to the tibial tubercle and used to apply a load of 150 N directed anteroposteriorly to the tibia to simulate the Lachman test. The knees then received constant axial compression and 3D knee kinematic data were collected by digitising the screw head positions in 30° flexion under each test condition. Measurements of tibial translation and rotation were made, first with the ACL intact, then after sequential cutting of the ACL bundles, and finally after complete division of the ACL. Two-way analysis of variance analysis was performed. During the Lachman test, in all knees and in all test conditions, lateral tibial translation exceeded that on the medial side. With an intact ACL, both anterior and lateral tibial landmarks translated significantly more than those on the medial side (p < 0.001). With sequential division of the ACL bundles, selective cutting of the posterolateral bundle (PLB) did not increase translation of any landmark compared with when the ACL remained intact. Cutting the anteromedial bundle (AMB) resulted in an increased anterior translation of all landmarks. Compared to the intact ACL, when the ACL was fully transected a significant increase in anterior translation of all landmarks occurred (p < 0.001). However, anterior tibial translation was almost identical after AMB or complete ACL division. We found that the AMB confers its most significant contribution to tibial translation during the Lachman test, whereas the PLB has a negligible effect on anterior translation. Section of the PLB had a greater effect on increasing the internal rotation of the tibia than the AMB. However, its contribution of a mean of 2.8° amplitude remains low. The clinical relevance of our investigation suggests that, based on anterior tibial translation only, one cannot distinguish between a full ACL and an isolated AMB tear. Isolated PLB tears cannot be detected solely by the Lachman test, as this bundle probably contributes more resistance to the pivot shift.

Guidelines and ethical considerations for housing and management of psittacine birds used in research.

The Psittaciformes are a large order of landbirds comprising over 350 species in about 83 genera. In 2009, 141 published studies implicated parrots as research subjects; in 31 of these studies, 483 individuals from 45 different species could be considered laboratory animals. Amazons and budgerigars were by far the most represented psittacine species. The laboratory research topics were categorized as either veterinary medicine and diagnostics (bacteriology, hematology, morphology, and reproduction; 45%) or behavioral and sensory studies (behavior, acoustics, and vision; 17%). Confinement of psittacine species for research purposes is a matter of concern as scientifically based species-specific housing guidelines are scarce. The aim of this article is to provide scientific information relevant to the laboratory confinement of Psittaciformes to promote the refinement of acquisition, housing, and maintenance practices of these birds as laboratory animals. We briefly discuss systematics, geographical distribution, legislation, and conservation status as background information on laboratory parrot confinement. The following section presents welfare concerns related to captive containment (including domestication status) and psittacine cognition. We then discuss considerations in the acquisition of laboratory parrots and review important management issues such as nutrition, zoonoses, housing, and environmental enrichment. The final section reviews indications of distress and compromised welfare.

Does combined posterior cruciate ligament and posterolateral corner reconstruction for chronic posterior and posterolateral instability restore normal knee function?

INTRODUCTION: Posterior cruciate ligament (PCL) injuries are frequently associated with posterolateral corner (PLC) damages. These complex lesions are most often poorly tolerated clinically. Adherence to sound biomechanical principles treating these complex lesions entails obtaining a functional PCL and reconstructing sufficient posterolateral stability. HYPOTHESIS: Surgical treatment of postero-posterolateral laxity (PPLL) re-establishes sufficient anatomical integrity to provide stability and satisfactory knee function. MATERIAL AND METHODS: In this retrospective, continuous, single-operator study, 21 patients were operated for chronic PPLL with combined reconstruction of the PCL and PLC and were reviewed with a minimum 1 year follow-up. The clinical and subjective outcomes were evaluated using the IKDC score. Surgical correction of posterior laxity was quantified clinically and radiologically on dynamic posterior drawer images (posterior Telos stress test and hamstrings contraction lateral view). RESULTS: The mean subjective IKDC score was 62.8 at the last follow-up versus a preoperative score of 54.5 (NS). Preoperatively, all were classified in groups C and D. Postoperatively, 13 patients out of 21 were classified in groups A and B according to the overall clinical IKDC score. The radiological gain in laxity was 51% on the hamstring contraction films and 67% on the posterior Telos images (p<0.05). DISCUSSION: The objective of surgical treatment is to re-establish anatomical integrity to the greatest possible extent. The clinical and radiological laxity results are disappointing in terms of the objectives but are in agreement with the literature. The subjective evaluation demonstrated that this operation can provide sufficient function for standard daily activities but not sports activities. LEVEL OF EVIDENCE: Level IV retrospective study.

Does reconstruction of isolated chronic posterior cruciate ligament injuries restore normal knee function?

INTRODUCTION: Isolated posterior laxity is most often cared for with conservative functional treatment. However, when there is pain or instability, surgical treatment can legitimately be proposed. The objective of this study was to assess the results of surgical treatment for chronic isolated posterior laxity. HYPOTHESIS: Surgical treatment of direct posterior laxity re-establishes sufficient anatomical integrity to stabilize and provide good function to the knee. MATERIAL AND METHODS: This was a retrospective, continuous, single-operator study. Eleven operated patients were retained for this study, all followed up a mean 20.9 months, with a minimum follow-up of 1 year. Subjective and clinical assessments were carried out using the International Knee Documentation Score (IKDC) score. Surgical correction of posterior laxity was measured clinically and radiologically. RESULTS: The subjective IKDC score increased from 53 preoperatively to 68.5 at the last follow-up (P=0.006). For the objective IKDC score, all knees were classified C or D preoperatively; at the last follow-up, six were A or B and none D. All the knees had preoperative Clancy grade 2 or 3 laxity; after surgery, there were three. According to the IKDC laxity score, eight knees were classified A or B at the last follow-up. The radiographic workup noted a 48.6% (P=0.05) posterior laxity correction on the TELOS test. DISCUSSION: Posterior cruciate ligament reconstruction provides partial correction of posterior laxity. However, the subjective result remains insufficient, providing acceptable function for daily life activities but not sports activities. LEVEL OF EVIDENCE: Level IV, retrospective study.

Psittacine birds as laboratory animals: refinements and assessment of welfare.

We address housing, refinements of husbandry, and some concerns regarding the use of parrots as laboratory animals. Because the duration of a project is most likely brief relative to the lifespan of the bird, among the most important goals is a well-established socialization program to maximize success of rehoming the birds after laboratory housing. We also present appropriate methods for catching and restraining parrots during experimental procedures. We discuss factors that contribute to appropriate laboratory and cage environments, such as the importance of cage location in the animal room as well as providing birds with suitable perching and enrichment devices. Finally, we review a few methods for scoring signs of compromised welfare in psittacine birds.

Intraperitoneal versus subcutaneous telemetry devices in young Mongolian gerbils (Meriones unguiculatus).

Radiotelemetry has become a very popular biotelemetric tool for measuring physiological parameters such as heart rate, blood pressure, body temperature and muscle activity, as well as general behavioural activity in undisturbed, freely moving animals. In most studies using this technique, adult subjects are used. However, sometimes an ontogenetic approach is required to clarify whether changes in one parameter are preceeded or followed by changes in another parameter. Tracking physiological changes in young, developing individuals could explain given states of these animals as adults. Implanting telemetry devices can be done subcutaneously and intraperitoneally, the former method posing less of a challenge on the animal and its recovery from surgery. Because telemetry will be used in weanling gerbils during subsequent studies, we needed to investigate whether subcutaneous implantation of telemetric devices is preferable to intraperitoneal surgery with respect to animal welfare. This is a technical paper describing anaesthetic and surgical techniques in detail during a pre-trial involving subcutaneous (n=10, aged 21-29 days) and intraperitoneal (n=10, aged 19-34 days) implantation of dummy telemetry transmitters (1.9 cm3, 3.6 g after shortening of leads) in weanling gerbils, Meriones unguiculatus. Body weight was measured and analysed over four-day intervals. Optimizing anaesthetic dosages was a first step in this pilot trial. This occurred during the first few subcutaneous implantations. Three animals died while anaesthetized during the subcutaneous procedure but none post-surgery. All animals survived anaesthesia during the intraperitoneal implantation, but two died in the first three days post-surgery. In the former method, the tension on the dermal sutures caused by the presence of the transmitters was too great, resulting in the animals opening the sutures by chewing them. The animals died during the latter procedure probably due to strangulation of the intestine by the excess lead that was coiled in the abdomen. Furthermore, placement of the exposed negative lead of the transmitter on the underlying muscle had to be done on the m. pectoralis transversus in order for it to stay in place as the animal developed. This paper showed that the implantation of a telemetric device in weanling gerbils is feasible and is best executed through the intraperitoneal technique.