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1.
Biochem Biophys Res Commun ; 459(3): 553-9, 2015 Apr 10.
Article in English | MEDLINE | ID: mdl-25749339

ABSTRACT

Recent studies have shown that OPN (osteopontin) plays critical roles in cell survival, differentiation, bio-mineralization, cancer and cardiovascular remodeling. However, its roles in the differentiation of brown adipocytes and the underlying mechanisms remain unclear. Therefore, the aim of this study was to investigate the roles of OPN in the brown adipogenesis and the underlying mechanisms. It was shown that the OPN successfully induced the differentiation of 3T3-L1 white preadipocytes into the PRDM16(+) (PRD1-BF1-RIZ1 homologous domain containing 16) and UCP-1(+) (uncoupling protein-1) brown adipocytes in a concentration and time-dependent manner. Also, activation of PI3K (phosphatidylinositol 3-kinase)-AKT pathway was required for the OPN-induced brown adipogenesis. The findings suggest OPN plays an important role in promoting the differentiation of the brown adipocytes and might provide a potential novel therapeutic approach for the treatment of obesity and related disorders.


Subject(s)
Adipocytes, White/cytology , Adipocytes, White/metabolism , Adipogenesis/physiology , Osteopontin/metabolism , Phosphatidylinositol 3-Kinases/metabolism , Proto-Oncogene Proteins c-akt/metabolism , 3T3-L1 Cells , Adipogenesis/genetics , Animals , Cell Differentiation , Integrin alphaVbeta3/metabolism , Mesenchymal Stem Cells/cytology , Mesenchymal Stem Cells/metabolism , Mice , Osteopontin/genetics , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Signal Transduction
2.
Eur J Pharmacol ; 754: 41-51, 2015 May 05.
Article in English | MEDLINE | ID: mdl-25687252

ABSTRACT

Vascular endothelial insulin resistance (IR) is a critically initial factor in cardiocerebrovascular events resulted from diabetes and is becoming a worldwide public health issue. Thiazolidinediones (TZDs) are clinical insulin-sensitizers acting through a canonical peroxisome proliferator-activated receptor gamma (PPARγ)-dependent insulin trans-activation pathway. However, it remains elusive whether there are other mechanisms. In current study, we investigated whether TZDs improve endothelial IR induced by high glucose concentration or hyperglycemia via a non-canonical PPARγ-dependent nuclear factor-kappa B (NF-κB) trans-repression pathway. Our results showed that pre-treatment with TZDs dramatically decrease the susceptibility of endothelial cell to IR, while post-treatment notably improve the endothelial IR both in vitro and in vivo. Moreover, TZDs substantially increase the levels of endothelial nitric oxide synthase (eNOS) and inhibitory κB alpha (IκBα), whereas decrease those of the phosphorylated inhibitory κB kinase alpha/beta (phosphor-IKKα/ß) and the cytokines including tumor necrosis factor alpha (TNFα), interleukin-6 (IL-6), soluble intercellular adhesion molecule-1 (sICAM-1) and soluble vascular cellular adhesion molecule-1 (sVCAM-1), suggesting that TZDs act indeed through a PPARγ-dependent NF-κB trans-repression pathway. These findings highlighted a non-canonical mechanism for TZDs to ameliorate endothelial IR which might provide a potential strategy to prevent and treat the diabetic vascular complications clinically.


Subject(s)
Endothelium, Vascular/drug effects , Insulin Resistance/physiology , NF-kappa B/metabolism , PPAR gamma/agonists , PPAR gamma/physiology , Animals , Cytokines/metabolism , Down-Regulation/physiology , Endothelium, Vascular/metabolism , Human Umbilical Vein Endothelial Cells , Humans , I-kappa B Kinase/metabolism , I-kappa B Proteins/metabolism , Male , NF-KappaB Inhibitor alpha , Nitric Oxide Synthase Type III/metabolism , Rats , Repression, Psychology , Signal Transduction/drug effects , Thiazolidinediones/pharmacology
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