ABSTRACT
Introduction: Aberrant microRNA (miRNA) expressions are often discovered in many life threatening diseases such as cancer. In particular, recent studies show combinations of miRNA levels have greater diagnostic accuracy as opposed to single miRNA levels. For point-of-care applications, rapid and sensitive isothermal amplification with loop-mediated isothermal amplification (LAMP) has gained significant interest. Method: We developed a cost-effective point-of-care testing (POCT) device for multiple miRNAs that can integrate miRNA signals into a single output. Results and Discussion: We demonstrate that the loop primers for LAMP can be broken and be used for miRNA detection. This split-LAMP approach provides a logic AND-gate output for two distinct miRNA inputs. We then show that this is potentially useable in point-of-care testing using pH-sensitive dye to give a rapid, colorimetric endpoint readout within 30 min. This novel logic gate approach can potentially be extended to multiple miRNAs such that there can be a powerful diagnostic concept for multiple short RNAs in a point-of-care rapid test.
ABSTRACT
Research on self-esteem reactivity has demonstrated that self-esteem fluctuates in response to daily stressor exposure, and the strength of this relationship varies between individuals. Drawing upon the positive link between objective socioeconomic status (SES) and self-esteem, how subjective SES influences self-esteem reactivity to daily stressor exposure was explored. Using a 7-day daily diary study, the current study (Nparticipants = 243, Ndays = 1651) adopted a multilevel analysis to demonstrate that subjective SES attenuated the within-person association between daily stressor exposure and daily self-esteem, even after controlling for demographics and objective indicators of SES. The interactions were also consistent across social stressors and non-social stressors. The findings provide evidence supporting the protective role of subjective SES in self-esteem reactivity to daily stressor exposure.
ABSTRACT
The negative consequences of smartphone usage have seen frequent discourse in popular media. While existing studies seek to resolve these debates in relation to executive functions, findings are still limited and mixed. This is partly due to the lack of conceptual clarity about smartphone usage, the use of self-reported measures, and problems related to task impurity. Addressing these limitations, the current study utilizes a latent variable approach to examine various types of smartphone usage, including objectively measured data-logged screen time and screen-checking, and nine executive function tasks in 260 young adults through a multi-session study. Our structural equation models showed no evidence that self-reported normative smartphone usage, objective screen time, and objective screen-checking are associated with deficits in latent factors of inhibitory control, task-switching, and working memory capacity. Only self-reported problematic smartphone usage was associated with deficits in latent factor task-switching. These findings shed light on the boundary conditions of the link between smartphone usage and executive functions and suggest that smartphone usage in moderation may not have inherent harms on cognitive functions.
Subject(s)
Executive Function , Smartphone , Young Adult , Humans , Self Report , Memory, Short-Term , CognitionABSTRACT
While smartphones have brought many benefits and conveniences to users, there is continuing debate regarding their potential negative consequences on everyday cognition such as daily cognitive failures. A few cross-sectional studies have found positive associations between smartphone use and cognitive failures. However, several research gaps remain, such as the use of cross-sectional designs, confounds related to stable individual differences, the lack of validity in self-report measures of smartphone use, memory biases in retrospective self-reports, and the lack of differentiation between smartphone checking and smartphone screen time. To simultaneously address the aforementioned shortcomings, the current study examined the within-person associations between various objective indicators of smartphone use and daily cognitive failures using a 7-day daily diary study. Multilevel modelling revealed that smartphone checking, but not total smartphone screen time, predicted a greater occurrence of daily cognitive failures at the within-person level. Surprisingly, we also found negative within-person associations between smartphone screen time for social- and tools-related applications and daily cognitive failures, suggesting that some types of smartphone use may temporarily benefit one's cognitive functioning. This finding demonstrates the importance of studying the specific functions of smartphone use and their differential cognitive consequences, as well as highlights the complex relations between smartphone use and cognition.
