ABSTRACT
BACKGROUND/AIM: Cervical cancer is one of the most common cancers worldwide and a major cause of cancer-related mortality among women. Previous studies have reported that microRNA-miR-187*, which is one of the non-coding parts of the genome producing small conserved ribonucleic acids, is associated with various cancers. In this study, we explored the function of miR-187* in cervical cancer cells. MATERIALS AND METHODS: miR-187* mimic, WWOX reporter constructs, siRNA and overexpression constructs were transfected into SiHa cells to investigate the function and regulatory mechanisms of miR-187*. RESULTS: Exogenous miR-187* was found to increase the oncogenic phenotypes of SiHa cells. The tumor suppressor gene WWOX is a novel target of miR-187* in SiHa cells. WWOX siRNA suppressed endogenous WWOX expression and increased the oncogenic phenotypes of SiHa cells. Exogenous WWOX expression was able to suppress the oncogenic phenotypes of SiHa cells and rescue cells from miR-187*-induced migration. CONCLUSION: miR-187* seems to enhance SiHa cervical cancer cell oncogenicity via suppression of the WWOX pathway.
Subject(s)
MicroRNAs/administration & dosage , Tumor Suppressor Proteins/antagonists & inhibitors , Uterine Cervical Neoplasms/enzymology , Uterine Cervical Neoplasms/genetics , WW Domain-Containing Oxidoreductase/antagonists & inhibitors , Cell Line, Tumor , Down-Regulation , Female , Genes, Tumor Suppressor , Humans , MicroRNAs/biosynthesis , MicroRNAs/genetics , RNA, Small Interfering/administration & dosage , Transfection , Tumor Suppressor Proteins/biosynthesis , Tumor Suppressor Proteins/genetics , Up-Regulation , Uterine Cervical Neoplasms/pathology , WW Domain-Containing Oxidoreductase/biosynthesis , WW Domain-Containing Oxidoreductase/geneticsABSTRACT
BACKGROUND: Preoperative tumor aggressiveness biomarkers may help surgeons decide the extent of an operation. However, whether serum angiogenetic factors can be used to predict the prognosis of patients with differentiated thyroid cancer is still unclear. METHODS: Seventy-six DTC patients were prospectively recruited. Preoperative serum samples were collected and measured for Tie-2, Ang-1, Ang-2, VEGF-A, and VEGF-D levels. The potential correlations between their serum levels and clinicopathologic features as well as their prognoses were analyzed. RESULTS: Older age (>45 years old) and higher VEGF-A serum levels were independent predictors of extrathyroidal extension. The VEGF-D serum level was an independent factor for lymph node metastases and VEGF-A was an independent factor for distant metastases. None of these serum angiogenetic factors were significantly different between patients who were disease free and those with recurrences. The presence of lymph node metastases was the only independent factor for recurrence over the 2-year follow-up. CONCLUSION: Preoperative serum VEGF-A and VEGF-D levels were significantly elevated in DTC patients with distant and lymph node metastases. These findings, when combined with other clinicopathological factors, may help in surgical decisions.
Subject(s)
Adenocarcinoma, Follicular/pathology , Biomarkers, Tumor/blood , Carcinoma, Papillary/pathology , Lymphatic Metastasis , Neoplasm Metastasis , Thyroid Neoplasms/pathology , Vascular Endothelial Growth Factor A/blood , Vascular Endothelial Growth Factor D/blood , Adenocarcinoma, Follicular/blood , Adenocarcinoma, Follicular/surgery , Adolescent , Adult , Age Factors , Aged , Aged, 80 and over , Angiopoietins/blood , Carcinoma, Papillary/blood , Carcinoma, Papillary/surgery , Child , Female , Humans , Lymph Nodes/pathology , Male , Middle Aged , Neoplasm Recurrence, Local/pathology , Prognosis , Prospective Studies , Receptors, TIE/blood , Thyroid Neoplasms/blood , Thyroid Neoplasms/surgery , Young AdultABSTRACT
BACKGROUND: The purpose of this study was to determine the anatomic features of the external branch of the superior laryngeal nerve (EBSLN) in Chinese adults. METHODS: We analyzed the anatomic distribution of the 86 EBSLNs in 43 cadavers. RESULTS: The incidences of the EBSLN in the thyroid area were 94.2% and 91.3% on the right and left sides, respectively. In accord with the Cernea classification, type 1 was 16.2%, type2a was 39.5%, and type 2 was 38.3%. There were no significant differences between the right and the left side. The high-risk position of the EBSLN was 77.8%. CONCLUSIONS: The inferior cornu of the thyroid cartilage was a reliable landmark in identifying the external branch of superior laryngeal nerve. Racial variations between the white and the Chinese should be taken into consideration for an explanation of the differences.
