[RAPD analysis of the populations of the malaria mosquitoes Anopheles superpictus and A. pulcherrimus in the malaria foci of the central Asia].

RAPD-analysis was used to estimate intraspecies-specific variability in malaria mosquitoes, the major vectors of malaria in Central Asia. The high heterogeneity of RAPD-loci was found in 7 populations of An. superpictus and 2 populations of An. pulcherrimus. Genetic distances between the populations were determined on the basis of analysis of the frequencies of 15 RAPD-loci. There was a genetic similarity of An. superpictus populations in the malaria foci of Tadjikistan with those in the neighboring Surkhandariya Region of Uzbekistan.

[Meiotic gynogenesis in the stellate, Russian sturgeon and sterlet]. / Meioticheskii ginogenez u sevriugi, russkogo osetra i sterliadi.

Diploid gynogenetic progenies were obtained in the stellate sturgeon Acipenser stellatus, Russian sturgeon A. gueldenstaedtii, and sterlet A. ruthenus by means of insemination of the eggs with UV-irradiated spermatozoa and suppression of the second meiotic division by heat shock. The gynogenetic nature of experimental fish was confirmed by RAPD-PCR analysis of DNA. Effective photoreactivation of UV-induced lesions of spermatozoa was shown in the case of illumination of the fertilized eggs with visible light. This phenomenon should be taken into account when determining the doses of irradiation that allow inactivation of the male chromosomes and incubating gynogenetic embryos. Gynogenetic stellate and Russian sturgeons are viable and can be reared in order to study the mechanism of sex determination in sturgeons.

[In vitro formation of triple-stranded DNA structures in the human alpha1-antitrypsin gene]. / Poluchenie in vitro trekhtsepochechnykh struktur v gene alpha1-antitripsina cheloveka.

We analyzed the possibility of triplex formation in the human alpha 1-antitrypsin gene. Conditions and nucleotide sequence dependence of triplex formation and thermostability of the product were determined.