ABSTRACT
Structural changes of eye chorioretinal complex were investigated in 40 adult male outbred albino rats after total transient cerebral ischemia using electron microscopy and morphometric analysis. Furthermore, the influence of a new sterically hindered phenolic antioxidant dibornol on these processes was estimated. Our studies demonstrated that total transient cerebral ischemia in rats resulted in the capillary thrombosis of the choriocapillary lamina of the uvea, structural disturbances of the blood-retinal barrier, degeneration of the retinal neurons and radial glia. Course administration of dibornol was shown to improve the microcirculation and to protect the retinal neuronal structures, pigment epithelium, and radial glia.
Subject(s)
Brain Ischemia/pathology , Camphanes/pharmacology , Central Serous Chorioretinopathy/pathology , Choroid/ultrastructure , Cresols/pharmacology , Retina/ultrastructure , Animals , Blood-Retinal Barrier/metabolism , Choroid/pathology , Male , Neurons/ultrastructure , Rats , Retina/physiopathology , Thrombosis/pathologyABSTRACT
Along with microangiopathy, one of the main causes of blindness in diabetic retinopathy consists of degeneration of retinal neurons. Electron microscopy and morphometric analysis were used to study structural changes in neurosensory cells, associative, and ganglion neurons in the retina in 30 while mongrel male rats with streptozotocin diabetes for two months and the effects of a new semisynthetic antioxidant 4-methyl-2,6-diisobornylphenol, a screened phenol, were evaluated. Destructive changes were found to affect the outer segments of neurosensory cells and ganglion neurons. The number density of neurosensory and ganglion cells decreased, and the proportion of these cells with pyknotic nuclei increased. 4-Methyl-2,6-diisobornylphenol had neuroprotective actions, preventing destructive changes to neurosensory cells and ganglion neurons.
Subject(s)
Camphanes/therapeutic use , Cresols/therapeutic use , Diabetes Mellitus, Experimental/complications , Diabetic Retinopathy/drug therapy , Neuroprotective Agents/therapeutic use , Retinal Neurons/drug effects , Animals , Camphanes/pharmacology , Cresols/pharmacology , Diabetes Mellitus, Experimental/pathology , Diabetic Retinopathy/etiology , Diabetic Retinopathy/pathology , Male , Neuroprotective Agents/pharmacology , Rats , Retinal Neurons/pathology , StreptozocinABSTRACT
Along with microangiopathy, degeneration of retinal neurons is one of the basic causes of blindness in patients with diabetic retinopathy. Using the electronic microscopy and morphometric analysis, the structural changes of neurosensory cells, associative and ganglionic retinal neurons were studied in 30 albino outbred male rats with long term (2 months) streptozotocin diabetes and the effect of a new semisynthetic antioxidant belonging to a group of strictly hindered phenols (4-methyl-2,6-diisobornylphenol) on these parameters was evaluated. In diabetic rats, the destructive changes of external segments of neurosensory cells and ganglionic retinal neurons were found. The numerical density of neurosensory and ganglionic cells was reduced, while the proportion of these cells with pyknotic nuclei was increased. 4-Methyl-2,6-diisobornylphenol demonstrated neuroprotective effect by preventing destructive changes of neurosensory cells and ganglionic retinal neurons.
