ABSTRACT
Passive sensors provide a new route for the characterization of concentration-dependent radiofrequency parameters with high reproducibility in real time. We propose a microfabricated resonator realized using integrated passive device technology for the sensitive detection and characterization of glucose. Experimental results verify the high performance of the proposed biosensor, because radiofrequency parameters such as resonance frequency (from 0.541 to 1.05 GHz) and reflection coefficient (from -34.04 to -24.11 dB) linearly vary in response to deionized water and subsequent iterative measurements of different glucose concentrations (from 50 to 250 mg dL-1). The biosensor has a very low limit of detection of 8.46 mg dL-1, a limit of quantitation of 25.63 mg dL-1, a minimum frequency sensitivity of 29 MHz, and a minimum magnitude sensitivity of 0.22 dB. Moreover, the coupling coefficient consistently decreases with the increasing glucose concentration. We also used the measured radiofrequency parameters to determine the unknown permittivity of glucose samples through mathematical modeling. A decreasing trend in the loss tangent and an increasing trend in the characteristic wave impedance were observed with the increase of glucose concentration. The reproducibility of the sensor was verified through iterative measurements on the same sensor surface and subsequent study of surface morphology.
ABSTRACT
Tremendous demands for sensitive and reliable label-free biosensors have stimulated intensive research into developing miniaturized radiofrequency resonators for a wide range of biomedical applications. Here, we report the development of a robust, reusable radiofrequency resonator based integrated passive device biosensor chip fabricated on a gallium arsenide substrate for the detection of glucose in water-glucose solutions and sera. As a result of the highly concentrated electromagnetic energy between the two divisions of an intertwined spiral inductor coupled with an interdigital capacitor, the proposed glucose biosensor chip exhibits linear detection ranges with high sensitivity at center frequency. This biosensor, which has a sensitivity of up to 199 MHz/mgmL(-1) and a short response time of less than 2 sec, exhibited an ultralow detection limit of 0.033 µM and a reproducibility of 0.61% relative standard deviation. In addition, the quantities derived from the measured S-parameters, such as the propagation constant (γ), impedance (Z), resistance (R), inductance (L), conductance (G) and capacitance (C), enabled the effective multi-dimensional detection of glucose.
