Subject(s)
Acetylmuramyl-Alanyl-Isoglutamine/analogs & derivatives , Acetylmuramyl-Alanyl-Isoglutamine/therapeutic use , Adjuvants, Immunologic/therapeutic use , Acetylmuramyl-Alanyl-Isoglutamine/administration & dosage , Acetylmuramyl-Alanyl-Isoglutamine/pharmacology , Adjuvants, Immunologic/administration & dosage , Adjuvants, Immunologic/pharmacology , Bacterial Infections/drug therapy , Bacterial Infections/prevention & control , Clinical Trials as Topic , Humans , Virus Diseases/drug therapy , Virus Diseases/prevention & controlABSTRACT
Hybridoma technique was used to obtain a clone producing IgG antibodies. Paired helical neurofilaments isolated from the brain of patients suffering from Alzheimer's disease were used as an antigen during preparation of the clone. Antibodies recognize neurons with neurofibrillary changes. Specificity of the obtained antibodies to paired helical filaments was proved immunohistochemically. Monoclonal antibodies obtained are likely to be useful in the determination of the role played by pathological proteins in mutually twisted filaments formation.
Subject(s)
Alzheimer Disease/pathology , Antibodies, Monoclonal/biosynthesis , Cerebral Cortex/ultrastructure , Immunoglobulin G/biosynthesis , Neurofibrillary Tangles/immunology , Alzheimer Disease/immunology , Animals , Antibodies, Monoclonal/immunology , Cerebral Cortex/immunology , Humans , Hybridomas/immunology , Immunization , Immunoglobulin G/immunology , Lymphocytes/immunology , Mice , Mice, Inbred BALB C , Multiple Myeloma/pathology , Spleen/cytologyABSTRACT
Solid-phase IEA was used to measure the level of autoantibodies to somatostatin in the blood serum of 44 schizophrenics and 24 healthy donors. The patients suffering from schizophrenia manifested a higher (p less than 0.01) level of immune responsiveness of the blood serum to somatostatin (0.665 +/- 0.03) as compared to the control group (0.509 +/- 0.05). The main contribution to the differences between the groups as regards the parameter measured is made by patients with malignant (0.810 +/- 0.10) and paranoid (0.773 +/- 0.08) schizophrenia whereas the patients' subgroup with slow-progressive schizophrenia did not differ from normal regarding the level of autoantibodies to somatostatin in the serum (0.504 +/- 0.03). These tentative data agree with a hypothesis of the involvement of autoimmune processes into the development of schizophrenia.
Subject(s)
Autoantibodies/analysis , Autoimmune Diseases/immunology , Schizophrenia/immunology , Somatostatin/immunology , Adolescent , Adult , Autoimmune Diseases/etiology , Chronic Disease , Humans , Schizophrenia/etiology , Schizophrenia, Paranoid/etiology , Schizophrenia, Paranoid/immunologyABSTRACT
Preliminary incubation of normal non-immune spleen lymphocytes from C57BL/6j and BALB/c mice with levamisole (1 . 10(-4) - 1 . 10(-5)M) for 45 min at 37 degrees C with subsequent wash-out resulted in potentiation of the cytotoxic effect with respect to allogenic target cells (L-cells). Simultaneous administration of lymphocytes and levamisole to a culture of target cells did not produce cytotoxic effect.
