ABSTRACT
This study investigated the recovery of H2 and CH4 from bagasse bioethanol fermentation residues (bagasse BEFR) using a two-stage bioprocess. In the hydrogen fermentation bioreactor (HFB), carbohydrate removal efficiency was maintained at 82-93% and the highest hydrogen yield was 8.24mL/gCOD at volumetric loading rate (VLR) of 80kgCOD/m(3)/day. The results indicated a positive correlation between hydrogen yield and butyrate-to-acetate ratio, which might be due to the mechanisms of lactate/acetate utilization for hydrogen production and acetogenesis occurring in the HFB. Remaining volatile fatty acids and alcohols in the HFB effluent were further utilized for methane production in methane fermentation bioreactor (MFB), in which the highest methane yield of 345.2mL/gCOD was attained at VLR of 2.5kgCOD/m(3)/day. Overall, the two-stage bioprocess achieved a maximum COD removal of 81% from bagasse BEFR, and converted 0.3% and 72.8% of COD in the forms of H2 and CH4, respectively.
Subject(s)
Biotechnology/methods , Cellulose/chemistry , Ethanol/metabolism , Fermentation , Hydrogen/analysis , Methane/biosynthesis , Acetates/analysis , Biofuels/analysis , Biological Oxygen Demand Analysis , Bioreactors , Butyrates/analysis , Carboxylic Acids/analysis , Wastewater/chemistry , Xylose/analysisABSTRACT
This study conducted batch experiments to evaluate the potential of butanol production from microalgae biodiesel residues by Clostridium acetobutylicum. The results indicated that with 90 g/L of glucose as the sole substrate the highest butanol yield of 0.2 g/g-glucose was found, but the addition of butyrate significantly enhanced the butanol yield. The highest butanol yield of 0.4 g/g-glucose was found with 60 g/L of glucose and 18 g/L of butyrate. Using microalgae biodiesel residues as substrate, C. acetobutylicum produced 3.86 g/L of butanol and achieved butanol yield of 0.13 g/g-carbohydrate via ABE fermentation, but the results indicated that approximately one third of carbohydrate was not utilized by C. acetobutylicum. Biological butanol production from microalgae biodiesel residues can be possible, but further research on fermentation strategies are required to improve production yield.
Subject(s)
Biofuels , Butanols/metabolism , Clostridium acetobutylicum/metabolism , Microalgae/metabolism , Butyrates/pharmacology , Clostridium acetobutylicum/drug effects , Fermentation/drug effects , Glucose/pharmacology , Hydrolysis , Microalgae/drug effects , Waste ProductsABSTRACT
This study evaluates a two-stage bioprocess for recovering hydrogen and methane while treating organic residues of fermentative bioethanol from rice straw. The obtained results indicate that controlling a proper volumetric loading rate, substrate-to-biomass ratio, or F/M ratio is important to maximizing biohydrogen production from rice straw bioethanol residues. Clostridium tyrobutyricum, the identified major hydrogen-producing bacteria enriched in the hydrogen bioreactor, is likely utilizing lactate and acetate for biohydrogen production. The occurrence of acetogenesis during biohydrogen fermentation may reduce the B/A ratio and lead to a lower hydrogen production. Organic residues remained in the effluent of hydrogen bioreactor can be effectively converted to methane with a rate of 2.8 mmol CH(4)/gVSS/h at VLR of 4.6 kg COD/m(3)/d. Finally, approximately 75% of COD in rice straw bioethanol residues can be removed and among that 1.3% and 66.1% of COD can be recovered in the forms of hydrogen and methane, respectively.