ABSTRACT
Progesterone and progesterone receptors (PGR) are known to play important roles in the pathophysiology of pelvic organ prolapse (POP). We investigated whether the PGR gene polymorphisms were associated with POP by conducting a case-control association study in 87 women with POP and 150 women without POP. Genotypes of the PGR gene polymorphisms (rs500760 and rs484389) were determined by polymerase chain reaction, followed by restriction fragment length polymorphism analysis. There was significant difference between women with and those without POP in the distribution of the PGR rs484389 genotypes evaluated. Using multivariable logistic regression, older age, increased body mass index, menopausal status, and PGR rs484389 genotype CT were significantly associated with POP. The present study shows that PGR genotype may be associated with POP.
Subject(s)
Receptors, Progesterone/genetics , Uterine Prolapse/genetics , Adult , Age Factors , Body Mass Index , Case-Control Studies , Chi-Square Distribution , Female , Genotype , Humans , Logistic Models , Menopause , Middle Aged , Polymerase Chain Reaction , Polymorphism, Genetic , Polymorphism, Restriction Fragment Length , Prognosis , RiskABSTRACT
OBJECTIVE: To investigate whether pelvic organ prolapse (POP) is associated with collagen 3 alpha 1 (COL3A1) polymorphisms and other factors. METHODS: A case-control association study was conducted with 84 women affected with POP and 147 controls. The genotypes of nucleotides COL3A1 rs1800255 and COL3A1 rs1801184 polymorphisms were ascertained by polymerase chain reaction and restriction fragment length polymorphism analysis. RESULTS: The distribution of the COL3A1 rs1800255 genotypes was significantly different among affected women and controls. Older age and incidence of COL3A1 rs1800255 genotype AA were significantly associated with risk of POP. CONCLUSION: There may be an association between COL3A1 genotype and risk of POP.
Subject(s)
Collagen Type III/genetics , Polymorphism, Single Nucleotide , Uterine Prolapse/genetics , Adult , Age Factors , Base Sequence , Case-Control Studies , Female , Genetic Predisposition to Disease , Genotype , Humans , Middle Aged , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Risk Factors , Uterine Prolapse/physiopathologyABSTRACT
Estrogen and estrogen receptors are known to play important roles in the pathophysiology of pelvic organ prolapse (POP). We investigated whether estrogen receptor alpha (ERalpha) gene polymorphisms were associated with POP risk by conducting a case-control association study in 88 women with POP and 153 women without POP. Genotypes of the ERalpha (ESR1) gene polymorphisms (rs17847075, rs2207647, rs2234693, rs3798577, and rs2228480) were determined by polymerase chain reaction, followed by restriction fragment length polymorphism analysis. There was significant difference between women with and those without POP in the distribution of the ESR1 rs2228480 genotypes evaluated. By using multivariable logistic regression, age and ESR1 rs2228480 genotype GA were significantly associated with POP risk. Although the sample size of women with POP studied is small, the present study shows that ERalpha genotype may be associated with POP risk.
Subject(s)
Estrogen Receptor alpha/genetics , Polymorphism, Single Nucleotide , Uterine Prolapse/genetics , Case-Control Studies , Female , Humans , Immunohistochemistry , Logistic Models , Risk Factors , Uterine Prolapse/epidemiologyABSTRACT
OBJECTIVE: Estrogen and estrogen receptors are known to play important roles in the pathophysiology of pelvic organ prolapse (POP). We investigated whether estrogen receptor beta (ERbeta) gene polymorphisms were associated with POP by conducting a case-control association study in 69 women with POP and 141 women without POP. STUDY DESIGN: Genotypes of the ERbeta gene polymorphisms (rs2987983, rs1271572, rs944459, rs1256049, and rs1255998) were determined by polymerase chain reaction, followed by restriction fragment length polymorphism analysis. Haplotyping analysis was used to determine the relationship among five polymorphisms in the ERbeta gene and POP. RESULTS: There was no significant difference between women with and those without POP in the distribution of any of the genotypes evaluated. In haplotype frequency estimation analysis, haplotype CGCGC was more prevalent in women with POP (16.7%) than in women without POP (8.9%) (p=0.011). Using multivariable logistic regression, age, parity and haplotype CGCGC were significantly associated with POP. CONCLUSION: Although the sample size of women with POP studied is small, the present study shows that ERbeta gene haplotype may be associated with POP.
