ABSTRACT
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ABSTRACT
Surveys for long-term monitoring programs managing natural resources often incorporate sampling design complexity. However, design weights are often ignored in trend models of data from complex sampling designs. Generalized random tessellation stratified samples of a simulated population of lakes are selected with various levels of survey design complexity, and three trend approaches are compared. We compare an unweighted trend model, linear regression models of the trend in design-based estimates of annual status, and a probability-weighted iterative generalized least squares (PWIGLS) approach with a linearization variance. The bias and confidence interval coverage of the trend estimate and the size and power of the trend test are used to evaluate weighted and unweighted approaches. We find that the unweighted approach often outperforms the other trend approaches by providing high power for trend detection and nominal confidence interval coverage of the true trend regression parameter. We also find that variance composition and revisit design structure affect the performance of the PWIGLS estimator. When a subpopulation exhibiting an extreme trend is sampled disproportionately to its occurrence in the population, the unweighted approach may produce biased estimates of trend with poor confidence interval coverage. We recommend considering variance composition and potential subpopulation trends when selecting sampling designs and trend analysis approaches.
Subject(s)
Environmental Monitoring/methods , Lakes/chemistry , Least-Squares Analysis , Nevada , Probability , Research Design , Surveys and Questionnaires , WaterABSTRACT
Acorn tannins may affect food preferences and foraging strategies of squirrels through effects on acorn palatability and digestibility and squirrel physiology. Captive eastern gray squirrels (Sciurus carolinensis) were fed 100% red oak (Quercus rubra) or white oak (Quercus alba) acorn diets to determine effects on intake, digestion, and detoxification activity. Red oak acorns had higher phenol and tannin levels, which may explain the lower dry matter intakes and apparent protein digestibilities and the higher glucuronidation activities observed in squirrels. Although the white oak acorn diet had lower apparent protein digestibilities than the reference diet, it did not suppress dry matter intake for a prolonged period or stimulate glucuronidation. Negative physiological effects of a 100% red oak acorn diet suggest gray squirrels may require other foods to dilute tannin intake and provide additional nutrients. To distinguish the roles of different tannin types in the observed effects of acorn diets on squirrels, squirrels were fed rat chow containing no tannins, 4% or 8% tannic acid (hydrolyzable tannin), or 3% or 6% quebracho (condensed tannin). Apparent protein digestibilities were reduced by tannic acid and quebracho diets. Only the 8% tannic acid diet tended to increase glucuronidation. Specific effects of tannins may largely depend on tannin type, composition, and source and on other nutritional and physiological factors.