Investigation and improvement of the problems which are caused by pretreating reused medical instruments after usage / 中国实用护理杂志

Objective To discuss the reason of the problem, which are caused by pretreating reused medical instruments after usage in each hospital clinical departments;ensure the washing quality for the reused medical instruments and prolonging the working life of them. Methods The execution rate, washing quality, damage of which pretreating reused medical instruments in clinical departments were counted The Clinic of Infection, Nursing Department and Central Sterile Supply Department observed and adopted questionnaire for information, and then formulated targeted guidance and improvement according to the information. Results The execution rate of pretreating reused medical instrument after usage reached from 64.6% to 99.4%, the qualified rate of instrument washing had improved from 93.0% to 99.7%,the damage rate of instruments had decreased from 1.84% to 0.74%,and there were significant differences(χ2=9 413.8,38 160.6,2 820.2,all P<0.05). Conclusions Pretreating reused medical instrument after usage correctly and timely,can not only ensure the washing quality for the medical instruments, but also prolong the working life of them. It ensures the application value of the medical instruments.

Epidermal growth factor receptor mutations in non-small cell lung cancer of tissue and peripheral blood by fluorescence quantitive polymerase chain reaction / 国际肿瘤学杂志

Objective To investigate gene mutations of the epidermal growth factor receptor of tumor tissue and peripheral blood in non-small-cell lung cancer, and to compare the consistency of EGFR mutations in tumor tissue and peripheral blood, fluorescence quantitive polymerase chain reaction (FQ-PCR) was performed according to standard procedure. Methods 112 peripheral blood and 87 tumor tissue of NSCLC were detected by FQ-PCR, among which 45 cases were analyzed using peripheral blood-tumor tissue matched samples. The relationship between clinical efficacy and EGFR mutations Was analyzed. Results Our results showed that there is 32 mutations in EGFR in 112(28.6%)of the patients'peripheral blood samples and 27 mutations in EGFR in 87(31.O%)tumor tissue. EGFR mutations were mainly found in adenocarcinoma and non-smokers in NSCLC (P<0.05). The consistency of EGFR mutation between tumor tissue and peripheal blood reached 71.4%. The clinical outcome of mutations Was significantly effective(P