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Objective@#To analyze the efficacy of HLA-haploidentical peripheral hematopoietic stem cell transplantation (haplo-PBSCT) following reduced intensity conditioning (RIC) regimen to treat the patients with hematological malignancies who were older than 50 years old.@*Methods@#Eighteen patients with hematological malignancies over 50 years were enrolled, including 8 male and 10 female patients. The median age of all patients was 52 (range: 50–66) years. Of them, 8 patients had acute myeloid leukemia (AML) , 2 chronic myelocytic leukemia (CML) , 5 myelodysplastic syndrome (MDS) , 2 acute lymphoblastic leukemia (ALL) , and 1 aggressive natural killer cell leukemia (ANKL) . All patients received fludarabine, cytarabine and melphalan with rabbit anti-human thymocyte globulin (FAB+rATG regimen) and transplanted with high dose non-T cell-depleted peripheral hematopoietic stem cells from donors. Enhanced graft versus host disease (GVHD) prophylaxis and infection prevention were administered.@*Results@#Fifteen days after transplantation, 16 patients achieved complete donor chimerism. One of them rejected the donor graft completely at thirty days after transplantation, and the other 2 patients had mixed chimerism 15 days after transplantation and converted to complete recipient chimerism at 30 days after transplantation. The cumulative incidence of acute GVHD (aGVHD) was 61.1% (95%CI49.6%-72.6%) . The incidence of grade Ⅱ-Ⅳ aGVHD was 35.4% (95%CI 21.1%-49.7%) , whereas grade III-IV was 13.8% (95%CI 4.7%-22.9%) . The 2-year cumulative incidence of chronic GVHD (cGVHD) rate was estimated at 38.2% (95%CI 25.5%-50.9%) . Patients were followed-up for a median of 14.5 months (range, 3-44 months) . The Kaplan Meier estimates of 2-year overall survival (OS) and disease-free survival (DFS) was 72.6% (95%CI 60.1%-85.1%) and 63.7% (95%CI 49.2%-78.2%) , respectively. The 2-year cumulative incidence of relapse and non-relapse-mortality (NRM) was 31.2% (95%CI 16.5%-45.9%) and 12.5% (95%CI 4.2%-20.8%) , respectively.@*Conclusion@#RIC-haplo-PBSCT protocol can achieve better results in patients with hematologic malignancies over 50 years old.
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Objective To understand the epidemiological characteristics of human infection with avian influenza A (H7N9) virus in China,and provide evidence for the prevention and control of human infection with H7N9 virus.Methods The published incidence data of human infection with H7N9 virus in China from March 2013 to April 2017 were collected.Excel 2007 software was used to perform the analysis.The characteristics of distribution of the disease,exposure history,cluster of the disease were described.Results By the end of April 2017,a total of 1 416 cases of human infection with H7N9 virus were confirmed in China,including 559 deaths,the case fatality rate was 39.5％.In 2016,the case number was lowest (127 cases),with the highest fatality rate (57.5％).The first three provinces with high case numbers were Zhejiang,Guangdong and Jiangsu.The median age of the cases was 55 years and the male to female ratio was 2.3 ∶ 1.Up to 66％ of cases had clear live poultry exposure history before illness onset,31％ of cases had unknown exposure history and only 3％ of the cases had no live poultry exposure history.There were 35 household clusters (5 in 2013,9 in 2014,6 in 2015,5 in 2016,10 in 2017),which involved 72 cases,accounting for 5％ of the total cases.Conclusions The epidemic of human infection with H7N9 virus in China during 2013-2017 had obvious seasonality and spatial distribution.There was limited family clustering.Infection cases were mostly related to poultry contact.
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Objective To understand the epidemiological characteristics of human infection with avian influenza A (H7N9) virus in China,and provide evidence for the prevention and control of human infection with H7N9 virus.Methods The published incidence data of human infection with H7N9 virus in China from March 2013 to April 2017 were collected.Excel 2007 software was used to perform the analysis.The characteristics of distribution of the disease,exposure history,cluster of the disease were described.Results By the end of April 2017,a total of 1 416 cases of human infection with H7N9 virus were confirmed in China,including 559 deaths,the case fatality rate was 39.5％.In 2016,the case number was lowest (127 cases),with the highest fatality rate (57.5％).The first three provinces with high case numbers were Zhejiang,Guangdong and Jiangsu.The median age of the cases was 55 years and the male to female ratio was 2.3 ∶ 1.Up to 66％ of cases had clear live poultry exposure history before illness onset,31％ of cases had unknown exposure history and only 3％ of the cases had no live poultry exposure history.There were 35 household clusters (5 in 2013,9 in 2014,6 in 2015,5 in 2016,10 in 2017),which involved 72 cases,accounting for 5％ of the total cases.Conclusions The epidemic of human infection with H7N9 virus in China during 2013-2017 had obvious seasonality and spatial distribution.There was limited family clustering.Infection cases were mostly related to poultry contact.
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OBJECTIVE:To establish a method for the content determination of carbohydrates in Osseous and cucumis extract injection. METHODS:Ion chromatography was conducted on the column of Dionex CarboPac PA20 with mobile phase of water-1 mol/L Sodium acetate solution-250 mmol/L Sodium hydroxide solution(gradient elution)at flow rate of 0.45 ml/min,column tem-perature was 35 ℃,and injection volume was 10 μl,detector was electrochemical analysis detector. RESULTS:The linear ranges of galactose,glucose,mannose,sucrose and fructose were 0.5-10.0μg/ml(r>0.998 0);RSDs of precision,stability and reproduc-ibility tests were lower than 2%;recovery was 94.8%-99.1%(RSD=0.52%-0.89%,n=9). CONCLUSIONS:The method is sim-ple,fast and accurate,and can be used for the determination of carbohydrates in Osseous and cucumis extract injection.
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Objective To establish a method to analyze the composition and content of monoses in the garlic polysaccharide by ion chromatography. Methods The monoses in the solution of garlic polysaccharide hydrolysised by trifluoroacetic acid were determined by amino PAC column, eluted by gradient hydroxide sodium and identified by ampere detector (AU as working electrode, Ag/AgCl as reference electrode). Results The garlic polysaccharide was mainly composed by arabinose, galactose, glucose, xylose and fructose. Good resolution could be achieved among the above mentioned five kinds of monoses. Their linear correlations over the investigated concentration were above 0.998, while the average recovery rates were in the range of 95%-105%. Conclusion The ion chromatography method is fast, accurate, simple and reliable, and can be applied in the content determination and quality control of garlic polysaccharide.
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Objective To study the clinical significance of lung function and allergen detection in the diagnosis of cough variant asthma (CVA) in children. Method Forty-three cases of chronic cough in children with bronchial provocation (diastolic) test and skin prick allergy test results were analyzed, CVA group was 23 cases and control group was 20 cases. Results The positive of bronchial provocation (diastolic) test in CVA group was 19 cases, significantly higher than that in control group (3 cases, χ2=14.745, P ＜ 0.01 ).The positive of skin prick allergy test in CVA group was 17 cases , significantly higher than that in control group (5 cases, χ2 = 10.243,P ＜0.05). The correlation analysis showed that bronchial provocation (diastolic) test and skin prick allergy test was significantly correlated (r =0.404,P＜0.01 ).Conclusion Bronchial provocation (diastolic) test has an important role in the diagnosis of CVA; skin prick allergy test has a supporting role on the CVA diagnosis;allergens affect on lung function, bronchial provocation (diastolic) test with the help of skin prick allergy test in children with cough variant asthma diagnosis.
