[Development of quantitative analyse method for determination of alkaloid cytisin in Spartium junceum L., growing in Georgia].

The aim of the research was to develop a method for quantitative determination of cytisine in Spartium junceum L. We used the above-ground parts of plants. In developing a method of analysis we used the method of 3-phase extraction. In this case the best results were obtained in the system: chopped raw material - water solution of ammonia - chloroform. In this case, the amount of alkaloids extracted almost entirely from the plant and goes into the chloroform phase. Evaluation of the results was carried out by the validation. The method for determination of cytisine in raw product was proposed. The method comprises the following steps-extraction of raw materials extracting chloroform phase and its evaporation, the translation of solids in methanol, the chromatographic separation cytisine and its fixation of the spectrophotometer method. The method is reproducible, has the required accuracy, is easy to analysis (less than 9 hours).

[Biopharmaceutic and technological investigation of vagina suppositories containing brilliant green].

The vaginal suppository composition, containing 0, 05, 0, 1 and 0, 5 of drug substance is presented. The release of drug substance was investigated. The influence of a number of the factors on the degree of release was investigated. Cocoa oil, confectionery fat and three different types of emulgator were used. According to gained results the average release degree was 73-76%, which is sufficient for effective action of a preparation. The technological parameters of suppositories including the temperature melting and hardening are determined. All parameters were at an acceptable level. The optimum composition of suppositories was determined. The optimal composition of suppositories was determined (base, emulgator, the content of active substance, mass of the suppository. The investigation proved the effectiveness of the preparation.