Subject(s)
Cat Diseases/parasitology , Filariasis/veterinary , Animals , Brugia , Cats , Male , ThailandABSTRACT
Jirds were vaccinated by three to five subcutaneous (SC) injections of infective larvae of Brugia pahangi which had been irradiated at 25, 45 or 90 krads from a 60Co source. They were challenged either SC or intraperitoneally. Vaccination with four doses of 50 larvae irradiated with 25 krads produced 49.3% resistance to IP challenge worms and 39.8% against SC challenge worms. Five doses of larvae irradiated with 45 krads produced 62% resistance to SC challenge. Three doses of larvae irradiated with 90 krads produced 74.9% resistance to SC challenge and five doses produced 76.2% resistance. The reasons why irradiated larvae produce resistance whereas normal larvae do not are discussed.
Subject(s)
Brugia/immunology , Elephantiasis, Filarial/prevention & control , Lymphedema/prevention & control , Vaccination , Vaccines, Attenuated , Animals , Brugia/radiation effects , Female , Gerbillinae , Larva , MaleABSTRACT
Jirds were given a chemoprophylactic inoculation of flubendazole (FMBZ) and then five injections of infective larvae of Brugia pahangi whilst still protected by the FMBZ. When the drug was thought to be non-effective the jirds (and controls) were given a challenge infection of B. pahangi larvae. By comparison with control jirds the treated-infected-challenged jirds had 40% fewer adult worms. The control treated-challenged jirds contained mostly sterile female worms showing that they were still partially protected by FMBZ but worms numbers were not significantly reduced as compared with untreated controls.
Subject(s)
Antinematodal Agents/therapeutic use , Benzimidazoles/therapeutic use , Brugia/immunology , Filariasis/immunology , Filarioidea/immunology , Mebendazole/therapeutic use , Animals , Brugia/growth & development , Female , Filariasis/parasitology , Filariasis/prevention & control , Gerbillinae , Immunity , Immunization , Larva/immunology , Male , Mebendazole/analogs & derivatives , MicrofilariaeABSTRACT
When jirds were infected with a single inoculum of 25-50 infective larvae of Brugia pahangi an overall mean recovery of adult worms of 44.5% (n = 41) was obtained. There was no difference in recoveries between male and female jirds. If jirds were repeatedly inoculated with larvae into the peritoneal cavity yields were only slightly reduced. Yields were 30.5% for 5 infections (n = 10), 26.7% for 10 infections (n = 8), 34.4% for 15 infections (n = 10) and 28.5% for 20 infections (n = 7). Twice as many worms were recovered from intraperitoneally inoculated jirds than from subcutaneously inoculated jirds.
