ABSTRACT
An uniform multi-levelled approach to immunodiagnosis of tuberculosis of different sites is proposed with regard to latest information on the local immune response based on the detection of tuberculosis antibodies in blood and biological fluids from anatomic lesion areas. If necessary, immunological diagnosis is specified by using a set of serological tests and Koch's provocative test, as well as data on specific T-lymphocyte proliferation.
Subject(s)
Antibodies, Bacterial/analysis , Mycobacterium tuberculosis/immunology , Serologic Tests , Tuberculosis/diagnosis , Algorithms , Complement Fixation Tests , Diagnosis, Differential , Female , Hemagglutination Tests , Humans , Male , Tuberculosis/immunologyABSTRACT
The spectrum of tuberculosis antibodies detectable by indirect hemagglutination test, complement consumption, passive hemaagglutilution test (PHAT), and enzyme-linked immunosorbent assay (EISA) in patients with urinary tract diseases (tuberculosis, nonspecific disorders) was studied. In untreated bacteria-isolating patients in the early phase of disease, antibodies were found in 61.8%, their different types were detectable at different rates so it is more advisable to explore responses by using several serological tests. During treatment there is an increase in antibody detection rates by ELISA. At this time only can PHAT and ELISA be made. High seropositive rates (85-100%) are ensured by PHAT and ELISA in new case in end-stage disease. The antibodies are detected in 75 of the pretreated patients with nephrotuberculosis largely by PHAT and ELISA. Examination of patients with nonspecific disease by ELISA clarifies the clinical status of these patients.
