ABSTRACT
A novel human gene hNA TL (Human NAT Like) was cloned by in silico cloning and RT-PCR. hNA TL cDNA is 1803bp in length with a 621bp coding region, and the Genbank accession No. is AY632082. hNA TL encodes a protein of 206 amino acid residues which containing a N-acetyltransferase domain. hNA TL is located on human chromosome 17q25.2 with 6 exons. Serial analysis of gene expression revealed that hNA TL was highly expressed in human brain and gonad, while hNA TL was expressed in heart, spleen and gonad of adult mouse. Whole-mount in situ hybridization showed that hNATL specifically expresses in E7.5 and E8.5 mouse embryo brains and in HH10 stage chicken embryo brain. These results suggest that hNATL may play an important role in the development of embryo brain and may also be important for function of adult brain and gonad.
Subject(s)
Acetyltransferases/genetics , Brain/metabolism , Chromosomes, Human, Pair 17/genetics , Gene Expression Profiling , Amino Acid Sequence , Animals , Base Sequence , Blotting, Northern , Brain/embryology , Chick Embryo , Chickens , Chromosome Mapping , Cloning, Molecular , DNA, Complementary/chemistry , DNA, Complementary/genetics , Exons/genetics , Gonads/metabolism , Humans , In Situ Hybridization , Mice , Molecular Sequence Data , N-Terminal Acetyltransferase A , N-Terminal Acetyltransferase E , Sequence Alignment , Sequence Analysis, DNAABSTRACT
The zinc-finger motif found in many transcription factors is thought to be important for human heart development and diseases. This study reports cloning and expression analysis of a novel human zinc finger protein ZNF18 cDNA. The ZNF18 cDNA is 2,767 bp in length encoding a 549-amino-acid protein that contains a SCAN-box, a KRAB box and five consecutive C2H2 zinc finger motifs. ZNF18 shows high similarity with mouse Zfp535 (identity 77%). The ZNF18 gene is located on human chromosome 17p12-p13 with 9 exons and 8 introns. ZNF18 was ubiquitously expressed in adult mouse tissues except heart as revealed by Northern blot. Whole-mount in situ hybridization showed that expression of ZNF18 in mouse embryos was very dynamic. Expression was predominantly in extraembryonic tissues of E7.5 mouse embryo. By E8.5, ZNF18 began to express in anterior of trunk, and became abundant in tail and heart at E9.0, especially in the embryo heart at E10.5. These expression results suggest that ZNF18 may play an important role in the development of embryo heart.
Subject(s)
Gene Expression Profiling , Gene Expression Regulation, Developmental , Kruppel-Like Transcription Factors/genetics , Myocardium/metabolism , Amino Acid Sequence , Animals , Base Sequence , Blotting, Northern , Chromosomes, Human, Pair 17/genetics , Cloning, Molecular , DNA, Complementary/genetics , Embryo, Mammalian/metabolism , Female , Humans , In Situ Hybridization , Male , Mice , Molecular Sequence Data , Pregnancy , RNA, Messenger/genetics , RNA, Messenger/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Sequence Homology, Amino Acid , Zinc FingersABSTRACT
ceg1 gene was a novel human gene, which was cloned by bioinformatics research and RT-PCR. It was a single exon gene and located on human chromosome 14. The length of the cDNA was 2050 bp. Bioinformatics analysis predicted a 1340 bp complete open reading frame (ORF) which encoded a 446 amino acid protein, containing an EGF-like and CLECT domain. We found that the homologous genes of ceg1 in mouse embryo and chicken embryo were specifically expressed in the brain by in-situ hybridization. The result of RT-PCR of the mature mouse organs showed it was widely expressed in many organs. The result indicates that ceg1 gene may have an essential role in the development of brain and the maintenance of the organs' normal function. The analysis of expression and function profile of ceg1 gene may provide valuable insights into the functions of ceg1 in the development and function of human body.
