Can International Nonsewered Sanitation Standards Help Solve the Global Sanitation Crisis?

To address one of the most severe global challenges affecting human health and the environment, two new voluntary product standards (ISO 30500 and ISO 31800) for nonsewered sanitation systems (NSSS) and fecal sludge treatment units (FSTUs) have been developed and published. While providing stringent voluntary product requirements for the containment and the treatment of human excreta with safe outputs (air, liquids, and solids), ISO 30500 and ISO 31800 make the inextricable connections between environmental emission thresholds, technical innovations, and sustainability aspects of NSSS and FSTUs. The purpose of this feature is to discuss these connections.

Membrane-Based In-Gel Loop-Mediated Isothermal Amplification (mgLAMP) System for SARS-CoV-2 Quantification in Environmental Waters.

Since the COVID-19 pandemic is expected to become endemic, quantification of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in ambient waters is critical for environmental surveillance and for early detection of outbreaks. Herein, we report the development of a membrane-based in-gel loop-mediated isothermal amplification (mgLAMP) system that is designed for the rapid point-of-use quantification of SARS-CoV-2 particles in environmental waters. The mgLAMP system integrates the viral concentration, in-assay viral lysis, and on-membrane hydrogel-based RT-LAMP quantification using enhanced fluorescence detection with a target-specific probe. With a sample-to-result time of less than 1 h, mgLAMP successfully detected SARS-CoV-2 below 0.96 copies/mL in Milli-Q water. In surface water, the lowest detected SARS-CoV-2 concentration was 93 copies/mL for mgLAMP, while the reverse transcription quantitative polymerase chain reaction (RT-qPCR) with optimal pretreatment was inhibited at 930 copies/mL. A 3D-printed portable device is designed to integrate heated incubation and fluorescence illumination for the simultaneous analysis of nine mgLAMP assays. Smartphone-based imaging and machine learning-based image processing are used for the interpretation of results. In this report, we demonstrate that mgLAMP is a promising method for large-scale environmental surveillance of SARS-CoV-2 without the need for specialized equipment, highly trained personnel, and labor-intensive procedures.

Onsite Graywater Treatment in a Two-Stage Electro-Peroxone Reactor with a Partial Recycle of Treated Effluent.

The efficacy of an uncoupled electro-peroxone (E-peroxone) prototype reactor system for the treatment of synthetic graywater is determined. The two-stage E-peroxone process integrates ozonation with the in situ production of hydrogen peroxide (H2O2) in a first stage reactor before ozone (O3) is converted via the peroxone reaction to a hydroxyl radical (•OH). The two-stage prototype reactor system allows for the generation of H2O2 via cathodic oxygen reduction in the first-stage reactor before mixing with O3 in the second-stage reactor. This approach prevents the degradation of polytetrafluoroethylene (PTFE) coated carbon cathodes by •OH that takes place in a single well-mixed reactor that combines electrochemical peroxide generation with O3. The dosage of H2O2 into the second-stage reactor is optimized to enhance graywater treatment. Under these conditions, the uncoupled E-peroxone system is capable of treating synthetic graywater with an initial chemical oxygen demand (COD0) of 358 mg O2/L, a total organic carbon (TOC0) of 96.9 mg/L, a biochemical oxygen demand (BOD0) of 162 mg O2/L, and a turbidity of 11.2 NTU. The two-stage electro-peroxone system can reduce the initial COD0 by 89%, the TOC0 by 91%, BOD0 by 86%, and the turbidity by 95% after 90 min of treatment. At this performance level, the reactor effluent is acceptable for discharge and for use in nonpotable applications such as toilet-water flushing. A portion of the effluent is recycled back into the first-stage reactor to minimize water consumption. Recycling can be repeated consecutively for four or more cycles, although the time required to achieve the desired H2O2 concentration increased slightly from one cycle to another. The two-stage E-peroxone system is shown to be potentially useful for onsite or decentralized graywater treatment suitable for arid water-sensitive areas.

Optimal design of an electrochemical reactor for blackwater treatment.

Electrolysis of blackwater for disinfection and nutrient removal is a portable and scalable technology that can lessen the need for cities to construct large-scale wastewater treatment infrastructure and enable the safe onsite reuse of blackwater. Several systems for treating wastewater from single toilets are described in the literature, but there are few examples of systems designed to use electrolysis to treat blackwater from nearby toilets, which is a situation more common in densely packed urban living environments. In order to scale a single toilet electrolysis system to one that could service multiple toilets, computational fluid dynamic analysis was used to optimize the electrochemical reactor design, and laboratory and field-testing were used to confirm results. Design efforts included optimization of the reactor shape and mixing to improve treatment efficiency, as well as automated cleaning and salt injection to reduce maintenance and service requirements. PRACTITIONER POINTS: Design of a reverse polarity mechanism to enable in situ electrode cleaning and improve long-term electrode performance. Optimization of a hopper design and drainpipe location to collect and remove flaking precipitates and mitigate maintenance issues. Design of an automated salt injection system to guarantee sufficient chloride levels for producing adequate chlorine residuals for consistent disinfection.

Phosphate Recovery from Human Waste via the Formation of Hydroxyapatite during Electrochemical Wastewater Treatment.

Electrolysis of toilet wastewater with TiO2-coated semiconductor anodes and stainless steel cathodes is a potentially viable onsite sanitation solution in parts of the world without infrastructure for centralized wastewater treatment. In addition to treating toilet wastewater, pilot-scale and bench-scale experiments demonstrated that electrolysis can remove phosphate by cathodic precipitation as hydroxyapatite at no additional energy cost. Phosphate removal could be predicted based on initial phosphate and calcium concentrations, and up to 80% total phosphate removal was achieved. While calcium was critical for phosphate removal, magnesium and bicarbonate had only minor impacts on phosphate removal rates at concentrations typical of toilet wastewater. Optimal conditions for phosphate removal were 3 to 4 h treatment at about 5 mA cm-2 (∼3.4 V), with greater than 20 m2 m-3 electrode surface area to reactor volume ratios. Pilot-scale systems are currently operated under similar conditions, suggesting that phosphate removal can be viewed as an ancillary benefit of electrochemical wastewater treatment, adding utility to the process without requiring additional energy inputs. Further value may be provided by designing reactors to recover precipitated hydroxyapatite for use as a low solubility phosphorus-rich fertilizer.

Design and preliminary implementation of onsite electrochemical wastewater treatment and recycling toilets for the developing world.

Self-contained toilet wastewater treatment system prototypes based on electrochemical oxidation of feces and urine using bi-layered semiconductor anodes ([Bi2O3] z [TiO2]1-z/Ir x Ta y O2/Ti) have been designed, constructed, and implemented in regions where access to proper and sufficient sanitation is limited. Prototypes were designed to fit in shipping containers in order to provide toilets and onsite wastewater treatment with clean water recycling. Units were designed to handle the waste of 25 users per day (or 130 L of toilet wastewater). The first prototype was tested on the Caltech campus (Pasadena, California) followed by improved second-generation prototypes that were subsequently installed in India (Ahmedabad, Gujarat and Kottayam, Kerala) and China (Yixing, Jiangsu) for open use in various public settings. The prototypes were able to provide for the disinfection of pathogens (<10 MPN Total coliforms and <1 MPN Fecal coliform indicator organisms per 100 mL), reduction of chemical oxygen demand (<100 mg O2 L-1), ammonia (<10 mg N L-1), and color at an average energy consumption of less than 180 W h per user per day. The treated wastewater was recycled for use as toilet flushing water.

Urine microbial fuel cells in a semi-controlled environment for onsite urine pre-treatment and electricity production.

Microbial fuel cell (MFC) systems have the ability to oxidize organic matter and transfer electrons to an external circuit as electricity at voltage levels of <1 V. Urine has been shown to be an excellent feedstock for various MFC systems, particularly MFCs inoculated with activated sludge and with a terracotta ceramic membrane separating carbon-based electrodes. In this article, we studied a MFC system composed of two stacks of 32 individual cells each sharing the same anolyte. By combining the current produced by the 32 cells connected in parallel and by adding the potential of both stacks connected in series, an average power density of 23 mW m-2 was produced at an effective current density of 65 mA m-2 for more than 120 days. [NH3], TIC, COD, and TOC levels were monitored frequently to understand the chemical energy conversion to electricity as well as to determine the best electrical configuration of the stacks. Archaeal and bacterial populations on selected anode felts and in the anolyte of both stacks were investigated as well. Indicator microorganisms for bacterial waterborne diseases were measured in anolyte and catholyte compartments to evaluate the risk of reusing the catholyte in a non-regulated environment.

Electrochemical disinfection of toilet wastewater using wastewater electrolysis cell.

The paucity of proper sanitation facilities has contributed to the spread of waterborne diseases in many developing countries. The primary goal of this study was to demonstrate the feasibility of using a wastewater electrolysis cell (WEC) for toilet wastewater disinfection. The treated wastewater was designed to reuse for toilet flushing and agricultural irrigation. Laboratory-scale electrochemical (EC) disinfection experiments were performed to investigate the disinfection efficiency of the WEC with four seeded microorganisms (Escherichia coli, Enterococcus, recombinant adenovirus serotype 5, and bacteriophage MS2). In addition, the formation of organic disinfection byproducts (DBPs) trihalomethanes (THMs) and haloacetic acids (HAA5) at the end of the EC treatment was also investigated. The results showed that at an applied cell voltage of +4 V, the WEC achieved 5-log10 reductions of all four seeded microorganisms in real toilet wastewater within 60 min. In contrast, chemical chlorination (CC) disinfection using hypochlorite [NaClO] was only effective for the inactivation of bacteria. Due to the rapid formation of chloramines, less than 0.5-log10 reduction of MS2 was observed in toilet wastewater even at the highest [NaClO] dosage (36 mg/L, as Cl2) over a 1 h reaction. Experiments using laboratory model waters showed that free reactive chlorine generated in situ during EC disinfection process was the main disinfectant responsible for the inactivation of microorganisms. However, the production of hydroxyl radicals [OH], and other reactive oxygen species by the active bismuth-doped TiO2 anode were negligible under the same electrolytic conditions. The formation of THMs and HAA5 were found to increase with higher applied cell voltage. Based on the energy consumption estimates, the WEC system can be operated using solar energy stored in a DC battery as the sole power source.

Effects of anodic potential and chloride ion on overall reactivity in electrochemical reactors designed for solar-powered wastewater treatment.

We have investigated electrochemical treatment of real domestic wastewater coupled with simultaneous production of molecular H2 as useful byproduct. The electrolysis cells employ multilayer semiconductor anodes with electroactive bismuth-doped TiO2 functionalities and stainless steel cathodes. DC-powered laboratory-scale electrolysis experiments were performed under static anodic potentials (+2.2 or +3.0 V NHE) using domestic wastewater samples, with added chloride ion in variable concentrations. Greater than 95% reductions in chemical oxygen demand (COD) and ammonium ion were achieved within 6 h. In addition, we experimentally determined a decreasing overall reactivity of reactive chlorine species toward COD with an increasing chloride ion concentration under chlorine radicals (Cl·, Cl2(-)·) generation at +3.0 V NHE. The current efficiency for COD removal was 12% with the lowest specific energy consumption of 96 kWh kgCOD(-1) at the cell voltage of near 4 V in 50 mM chloride. The current efficiency and energy efficiency for H2 generation were calculated to range from 34 to 84% and 14 to 26%, respectively. The hydrogen comprised 35 to 60% by volume of evolved gases. The efficacy of our electrolysis cell was further demonstrated by a 20 L prototype reactor totally powered by a photovoltaic (PV) panel, which was shown to eliminate COD and total coliform bacteria in less than 4 h of treatment.