Multimodal Device for Real-Time Monitoring of Skin Oxygen Saturation and Microcirculation Function.

The present study introduces a recently developed compact hybrid device for real-time monitoring of skin oxygen saturation and temperature distribution. The prototype involves a snapshot hyperspectral camera, multi-wavelength illuminator, thermal camera, and built-in computer with custom-developed software. To validate this device in-vivo we performed upper arm vascular occlusion on eight healthy volunteers. Palm skin oxygen saturation maps were analyzed in real-time using k-means segmentation algorithm and two-layer optical diffuse model. The prototype system demonstrated a satisfying performance of skin hyperspectral measurements in the spectral range of 507-625 nm. The results confirmed the reliability of the proposed system for in-vivo assessment of skin hemoglobin saturation with oxygen and microcirculation.

Magnetic microrods as a tool for microrheology.

Dynamics of superparamagnetic rods in crossed constant and alternating magnetic fields as a function of field frequency are studied and it is shown that above the critical value of the amplitude of the alternating field the rod oscillates around the direction of the alternating field. The fit of the experimentally measured time dependence of the mean orientation angle of the rod allows one to determine the ratio of magnetic and viscous torques which act on the rod. The protocol of microrheological measurements consists of recording the dynamics of the orientation of the rod when the magnetic field is applied at an angle to the rod and observing its relaxation due to the accumulated elastic energy after the field is switched off. The microrheological data obtained are in reasonable agreement with the macrorheological measurements.