ABSTRACT
The HPLC technique for the isolation and quantitation of 5-fluoro-2-(5'-nitro-2'-furyl)benzimidazole (F-O-NO2), previously described by the same Authors, in biological samples from mice is reported. Evidence is given that the highest biological concentration and the time needed for reaching it depend both on the dose and on the administration route. In fact, almost the same maximal biological concentration of F-O-NO2 (about 0.18 micrograms/mouse) is reached after 60 min from either 40 mg/Kg IP or 200 mg/Kg os drug administration, whereas the maximal biological concentration of F-O-NO2 (5.75 micrograms/mouse) is reached after 30 min from 120 mg/Kg IP drug administration.
Subject(s)
Anti-Infective Agents/analysis , Benzimidazoles/analysis , Animals , Anti-Infective Agents/blood , Anti-Infective Agents/pharmacokinetics , Benzimidazoles/blood , Benzimidazoles/pharmacokinetics , Chromatography, High Pressure Liquid , Male , Mice , Organ Size , Tissue DistributionABSTRACT
The kinetics of hydrolysis of indomethacin and related compounds was studied in an alkaline medium at 25 degrees C in the presence of anionic (sodium dodecyl sulfate) and cationic (hexadecyltrimethylammonium bromide) surfactants. The rate-surfactant profiles for rate inhibition in the presence of sodium dodecyl sulfate and rate enhancement in the presence of hexadecyltrimethylammonium bromide were analyzed in terms of the current theory of micellar effects.
Subject(s)
Indomethacin/analysis , Cetrimonium , Cetrimonium Compounds , Hydrolysis , Indomethacin/analogs & derivatives , Kinetics , Micelles , Models, Chemical , Sodium Dodecyl SulfateABSTRACT
The kinetics of the hydrolysis of indomethacin and related compounds were studied in an alkaline medium at 25 degrees. The pseudo-first-order rate constants were evaluated from log absorbance versus time plots in the ultraviolet. These compounds showed a second-order rate constant at low concentrations of hydroxide ion and a first-order rate constant at higher concentrations of hydroxide ion.
