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1.
FEBS Lett ; 500(1-2): 99-104, 2001 Jun 29.
Article in English | MEDLINE | ID: mdl-11434934

ABSTRACT

Interleukin-2 (IL-2) regulates the proliferation and homeostasis of lymphocytes through the coordinated activation of distinct signaling pathways. Deletion of the acidic-rich domain of the IL-2 receptor beta chain (IL-2Rbeta) prevents association of Src tyrosine kinases to the receptor, as well as IL-2-induced Akt activation. Cells bearing this deletion (BafbetaDeltaA) maintain full proliferation in response to IL-2 both in vivo and in vitro, suggesting that those pathways are dispensable for this important function of IL-2. In this study, we re-examined phosphatidylinositol-3 kinase (PI3K) activation in BafbetaDeltaA cells and found that, in BaF/3 IL-2RbetaDeltaA cells, deletion of the acidic domain induced constitutive activation of the receptor-associated PI3K activity. This, in turn, was responsible for the higher basal Akt activity observed in cells expressing this deletion. Based on these data, and since pharmacological abrogation of PI3K activity prevented IL-2-driven cell proliferation of BafbetaDeltaA cells, we conclude that the PI3K/Akt pathway is still functionally relevant in cells bearing this mutation. Moreover, we show that the PI3K-induced signals are, at least in part, responsible for c-myc expression. In conclusion, we have used this model to better identify those signals that are integral components of the molecular mechanisms responsible for IL-2-regulated cell proliferation.


Subject(s)
Phosphatidylinositol 3-Kinases/metabolism , Protein Serine-Threonine Kinases , Receptors, Interleukin-2/metabolism , Animals , Cell Division/drug effects , Cell Division/physiology , Enzyme Activation , Enzyme Inhibitors/pharmacology , Gene Deletion , Interleukin-2/metabolism , Interleukin-2/physiology , Mice , Phosphoinositide-3 Kinase Inhibitors , Precipitin Tests , Protein Structure, Tertiary , Proto-Oncogene Proteins/metabolism , Proto-Oncogene Proteins c-akt , Proto-Oncogene Proteins c-myc/biosynthesis , Receptors, Interleukin-2/chemistry , Signal Transduction , Transfection , src-Family Kinases/metabolism
2.
J Immunol ; 163(2): 708-14, 1999 Jul 15.
Article in English | MEDLINE | ID: mdl-10395661

ABSTRACT

Stimulation via IL-2R ligation causes T lymphocytes to transit through the cell cycle. Previous experiments by our group have demonstrated that, in human T cells, IL-2 binding induces phosphatidic acid production through activation of the alpha isoform of diacylglycerol kinase. In this study, using the IL-2-dependent mouse T cell line CTLL-2, we demonstrate that pharmacological inhibition of IL-2-induced diacylglycerol kinase activation is found to block IL-2-induced late G1 to S transition without affecting cell viability. Herein, we demonstrate that diacylglycerol kinase inhibition has a profound effect on the induction of the protooncogenes c-myc, c-fos, and c-raf by IL-2, whereas expression of bcl-2 and bcl-xL are not affected. When the IL-2-regulated cell cycle control checkpoints are examined in detail, we demonstrate that inhibition of diacylglycerol kinase activation prevents IL-2 induction of cyclin D3 without affecting p27 down-regulation. The strict control of cell proliferation exerted by phosphatidic acid through activation of diacylglycerol kinase is independent of other well-characterized IL-2R-derived signals, such as the phosphatidylinositol-3 kinase/Akt pathway, indicating the existence of a different and important mechanism to control cell division.


Subject(s)
Cell Cycle Proteins , Diacylglycerol Kinase/antagonists & inhibitors , G1 Phase/immunology , Interleukin-2/physiology , Phosphatidylinositol 3-Kinases/physiology , Protein Serine-Threonine Kinases , S Phase/immunology , T-Lymphocytes, Cytotoxic/enzymology , Tumor Suppressor Proteins , Animals , Cell Cycle/drug effects , Cell Cycle/immunology , Cell Line , Culture Media, Serum-Free , Cyclin D3 , Cyclin-Dependent Kinase Inhibitor p27 , Cyclins/metabolism , Diacylglycerol Kinase/metabolism , Enzyme Activation/drug effects , Enzyme Activation/immunology , Enzyme Inhibitors/pharmacology , G1 Phase/drug effects , Gene Expression Regulation/drug effects , Gene Expression Regulation/immunology , Interleukin-4/antagonists & inhibitors , Interleukin-4/physiology , Mice , Microtubule-Associated Proteins/metabolism , Phosphatidylinositol 3-Kinases/metabolism , Piperidines/pharmacology , Proto-Oncogene Proteins/metabolism , Proto-Oncogene Proteins c-akt , Proto-Oncogenes/drug effects , Proto-Oncogenes/immunology , Quinazolines/pharmacology , Quinazolinones , Retinoblastoma Protein/metabolism , S Phase/drug effects , T-Lymphocytes, Cytotoxic/cytology , T-Lymphocytes, Cytotoxic/drug effects , T-Lymphocytes, Cytotoxic/metabolism
3.
Eur J Immunol ; 29(4): 1158-67, 1999 04.
Article in English | MEDLINE | ID: mdl-10229082

ABSTRACT

IL-2 binding to its high-affinity receptor regulates signaling events that control both lymphocyte cell survival and cell cycle progression. Although many studies have examined the mechanisms by which IL-2 regulates cell growth, few studies have dissected the pathways involved in promoting cell survival or the coupling of these pathways to the receptor. In the present study, using the pre-B cell line Baf-B03 transfected with a truncated form of the IL-2 receptor (IL-2R) beta chain, we demonstrate that IL-2-dependent cell survival requires only the N-terminal 350 amino acids of the IL-2Rbeta chain. IL-2-dependent survival of cells expressing the truncated receptor correlates with increases in receptor-associated phosphatidylinositol 3-kinase (PI3K) activity and expression of Bcl-X(L), but not with changes in c-Myc expression or proliferation. Inhibition of the PI3K pathway in these cells, but not in cells expressing the wild-type receptor, has a marked effect on the capacity of IL-2 to prevent cell death and diminishes the Bcl-X(L) response. The requirement for IL-2-induced PI3K activity in suppressing the onset of apoptotic cell death is discussed.


Subject(s)
Milk Proteins , Proto-Oncogene Proteins c-bcl-2/analysis , Receptors, Interleukin-2/physiology , Apoptosis , Cell Survival , DNA-Binding Proteins/physiology , Humans , Interleukin-2/pharmacology , Interleukin-3/pharmacology , Phosphatidylinositol 3-Kinases/physiology , STAT5 Transcription Factor , Trans-Activators/physiology , bcl-X Protein
4.
J Biol Chem ; 272(41): 25706-12, 1997 Oct 10.
Article in English | MEDLINE | ID: mdl-9325295

ABSTRACT

To elucidate the physiological role of human stromelysin-3 (hST-3) in tumor progression and/or wound healing, insulin-like growth factor-binding protein-1 (IGFBP-1) was analyzed as a potential physiological substrate. hST-3 proteolysis generates two fragments of 16 and 9 kDa that react with IGFBP-1 monoclonal antibody, although they do not bind insulin-like growth factor-I (IGF-I) in ligand blot. N-terminal sequencing shows that hST-3 cleaves IGFBP-1 at the His140-Val141 bond located in the IGFBP-1 midregion. We show that IGFBP-1 inhibits IGF-I-induced survival and proliferation of BAF/3 cells, as well as IGF-I-mediated activation of phosphatidylinositol 3-kinase (PI 3-K). Co-incubation of the IGF-I. IGFBP-1 complex with hST-3 restores IGF-I-induced proliferation and PI 3-K kinase activity in these cells. BAF/3 proliferation is significantly increased with the hST-3-treated IGF-I.IGFBP-1 complex compared with that obtained using IGF-I alone. To produce this enhanced proliferation, IGF-I must bind to IGFBP-1 before hST-3 proteolysis, demonstrated using an IGF-I variant that does not bind IGFBP. IGFBP-1 also inhibits IGF-I-induced proliferation of the MCF-7 breast adenocarcinoma, and this inhibition was not seen in hST-3-transfected MCF-7 cells. Such proteolysis may thus play a role in in vivo tumor progression. These results indicate that hST-3 may regulate IGF-I bioavailability by proteolyzing IGFBP, thus favoring cell survival and proliferation.


Subject(s)
Insulin-Like Growth Factor Binding Protein 1/metabolism , Metalloendopeptidases/metabolism , Humans , Hydrolysis , Insulin-Like Growth Factor I/metabolism , Matrix Metalloproteinase 11 , Metalloendopeptidases/genetics , Molecular Weight , Peptide Mapping , Phosphatidylinositol 3-Kinases/metabolism , Signal Transduction , Transfection , Tumor Cells, Cultured
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