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3.
Eur J Neurol ; 25(6): 833-840, 2018 06.
Article in English | MEDLINE | ID: mdl-29489040

ABSTRACT

BACKGROUND AND PURPOSE: Ataxia-telangiectasia (A-T) is a rare neurodegenerative disease, due to A-T mutated (ATM) gene mutations, which typically presents with signs of progressive neurological dysfunction, cerebellar ataxia and uncoordinated movements. A-T severely affects patients' quality of life. Successful treatment options are still not available. The aim of this multicenter study, performed with a blind evaluation procedure, was to define the minimal effective dosage of oral betamethasone, thus preventing the occurrence of side effects. METHODS: Nine A-T patients were enrolled to receive betamethasone at increasing dosages of 0.001, 0.005 and 0.01 mg/kg/day. Neurological assessment and the evaluation of quality of life were performed through the Scale for the Assessment and Rating of Ataxia and the Italian version of the Childhood Health Assessment Questionnaire (CHAQ) at each time-point. The drug safety profile was evaluated. Patients were categorized as responders, partial responders and non-responders. RESULTS: Four of nine patients had a benefit at a dose of 0.005 mg/kg/day of oral betamethasone. Using the higher dosage, only one additional patient had a positive response. Conversely, a daily dose of 0.001 mg/kg was ineffective. A correlation between the serum adrenocorticotropic hormone levels and the clinical response was observed. Five of 30 CHAQ items improved in four patients. CONCLUSIONS: These data suggest that a short-term betamethasone oral treatment, at a daily dosage of 0.005 mg/kg, is effective in some patients. Pre-existing risk factors for side effects should be taken into account before therapy.


Subject(s)
Ataxia Telangiectasia/drug therapy , Betamethasone/administration & dosage , Glucocorticoids/administration & dosage , Adolescent , Betamethasone/therapeutic use , Child , Child, Preschool , Dose-Response Relationship, Drug , Female , Glucocorticoids/therapeutic use , Humans , Male , Phenotype , Quality of Life , Treatment Outcome , Young Adult
5.
Eur Phys J C Part Fields ; 76(6): 349, 2016.
Article in English | MEDLINE | ID: mdl-28280440

ABSTRACT

Large arrays of aligned carbon nanotubes (CNTs), open at one end, could be used as target material for the directional detection of weakly interacting dark matter particles (WIMPs). As a result of a WIMP elastic scattering on a CNT, a carbon ion might be injected in the body of the array and propagate through multiple collisions within the lattice. The ion may eventually emerge from the surface with open end CNTs, provided that its longitudinal momentum is large enough to compensate energy losses and its transverse momentum approaches the channeling conditions in a single CNT. Therefore, the angle formed between the WIMP wind apparent orientation and the direction of parallel carbon nanotube axes must be properly chosen. We focus on very low ion recoil kinetic energies, related to low mass WIMPs ([Formula: see text] GeV) where most of the existing experiments have low sensitivity. Relying on some exact results on two-dimensional lattices of circular obstacles, we study the low energy ion motion in the transverse plane with respect to CNT directions. New constraints are obtained on how to devise the CNT arrays to maximize the target channeling efficiency.

9.
Eur J Neurol ; 18(4): 564-70, 2011 Apr.
Article in English | MEDLINE | ID: mdl-20840352

ABSTRACT

BACKGROUND: Ataxia-telangiectasia (A-T) is a non-curable neurodegenerative disorder, associated with progressive neurological dysfunction, oculocutaneous telangiectasia, immunodeficiency, predisposition to cancer and radiosensitivity. A recent study documented improvement in neurological symptoms after a short-term therapy with betamethasone in patients with A-T. Aim of this study was to evaluate the minimum therapeutically effective dosage of betamethasone on neurological symptoms of A-T. METHODS: Six responsive patients with A-T, received two 20-day cycles of oral betamethasone at 0.01 and 0.03 mg/kg/day (10% and 30% of the previously used full dosage), each followed by a 20-day washout period. Clinical and laboratory evaluations were carried out at T0 and at the end of each cycle. Neurological assessment was performed through the Scale for the Assessment and Rating of Ataxia (SARA). The glucocorticoid-induced leucine zipper (GILZ) and glucocorticoid receptor (GR) RNA expression were evaluated before and during the trial through real-time PCR. RESULTS: SARA scores significantly improved in all patients at the dosage of 0.03 mg/kg/day. In particular, three patients exhibited an improvement in 5/8 variables and two patients of 7 and 8 variables, respectively. Furthermore, the clinical improvement was already evident after the lower dosage. The basal GILZ and GR RNA expression were significantly lower in patients than in controls. GILZ expression increased in all patients after the beginning of the therapy, whereas no correlation between GR and the response was found. CONCLUSION: Our data indicate that betamethasone is effective in A-T at a minimal dosage and that GILZ may be a useful biomarker of the clinical response. This study provides Class IIIA evidence that betamethasone at very low dosage is effective in improving neurological signs of patients affected with ataxia-telangiectasia.


Subject(s)
Anti-Inflammatory Agents/administration & dosage , Ataxia Telangiectasia/drug therapy , Betamethasone/administration & dosage , Adolescent , Biomarkers/analysis , Child , Dose-Response Relationship, Drug , Female , Humans , Male , Receptors, Glucocorticoid/biosynthesis , Reverse Transcriptase Polymerase Chain Reaction , Transcription Factors/biosynthesis , Treatment Outcome , Young Adult
10.
Eur J Neurol ; 16(6): 755-9, 2009 Jun.
Article in English | MEDLINE | ID: mdl-19475758

ABSTRACT

BACKGROUND AND PURPOSE: Ataxia-telangiectasia (A-T) is a rare autosomal recessive disorder caused by alterations of the A-T mutated (ATM) gene. Although A-T is a non-curable disease, we, previously, documented a clear improvement of cerebellar functions during a short-term betamethasone trial. The aim of this study was to define the underlying biochemical mechanism. METHODS: In six A-T patients receiving a short-term steroid therapy, intracellular glutathione (GSH) levels were evaluated with a colorimetric assay. The lipid peroxidation level and reactive oxygen species (ROS) production were evaluated using commercial assays. All the parameters were compared with the improvement of cerebellar functions expressed as delta (Delta) of the Scale for the Assessment and Rating of Ataxia (SARA). RESULTS: We observed an inverse correlation between Delta SARA and the severity of cerebellar atrophy and between the latter and basal GSH values. Four of the five patients with the highest Delta SARA also had the highest GSH values. Moreover, even though basal ROS values were comparable in patients and controls, in the only patient studied at different time-points of therapy, a remarkable reduction in ROS levels was documented. CONCLUSION: We suggest that antioxidative mechanisms play a role in favouring the improvement of cerebellar functions observed in A-T patients receiving a short-term betamethasone trial.


Subject(s)
Ataxia Telangiectasia/drug therapy , Ataxia Telangiectasia/pathology , Betamethasone/pharmacology , Cerebellar Diseases/drug therapy , Cerebellar Diseases/pathology , Oxidative Stress/drug effects , Adolescent , Adult , Anti-Inflammatory Agents/pharmacology , Anti-Inflammatory Agents/therapeutic use , Antioxidants/metabolism , Ataxia Telangiectasia/physiopathology , Atrophy/drug therapy , Atrophy/metabolism , Atrophy/pathology , Betamethasone/therapeutic use , Cells, Cultured , Cerebellar Diseases/physiopathology , Cerebellum/drug effects , Cerebellum/metabolism , Cerebellum/pathology , Child , Child, Preschool , Disease Progression , Female , Glutathione/analysis , Glutathione/metabolism , Humans , Lipid Peroxidation/drug effects , Lipid Peroxidation/physiology , Male , Oxidative Stress/physiology , Reactive Oxygen Species/antagonists & inhibitors , Reactive Oxygen Species/metabolism , Severity of Illness Index , Treatment Outcome , Young Adult
11.
Med Lav ; 97(6): 749-61, 2006.
Article in Italian | MEDLINE | ID: mdl-17219764

ABSTRACT

BACKGROUND: The problem of the assessment of noise annoyance in the workplace has long been a topic of discussion. Several studies led to the definition of a set of descriptors suitable to describe noise in the workplace and its effects in terms of annoyance of the subjects exposed. OBJECTIVES: The aim of the study was to analyse the performance of different indices in terms of correlation with subjective sensation, taking into account those indices which combine both the intensity and the spectral quality of noise. METHOD: A noise survey was carried out in ten ofice buildings, covering 85 rooms. Objective measurements of environmental noise were made together with subjective evaluations collected through questionnaires. Subjective ratings were then averaged and correlated with objective parameters. Linear regression analyses were performed in order to assess the reliability of objective noise indices. RESULTS: The most important sources of noise were face-to-face and telephone conversations. All spectra had excess high frequency content. Regression analysis showed that under these conditions most noise indics provide a reliable prediction of subjective annoyance. However, the best performing parameter was the A-weighted equivalent sound pressure level followed by the Combined Noise Index which combines noise intensity and its spectral quality.


Subject(s)
Noise, Occupational , Acoustics , Adolescent , Adult , Aged , Data Collection , Female , Humans , Linear Models , Male , Middle Aged , Models, Theoretical , Noise, Occupational/adverse effects , Occupations , Psychoacoustics , Regression Analysis , Surveys and Questionnaires , Telephone
12.
Phys Rev E Stat Nonlin Soft Matter Phys ; 72(2 Pt 2): 026139, 2005 Aug.
Article in English | MEDLINE | ID: mdl-16196676

ABSTRACT

We study the kinetics of domain growth in the Ising model with nonconserved dynamics under the action of a stochastic driving field that mimics the action of a shear flow. At late times, we found multistriped configurations with constant transversal size and linear growth in the direction of the flow. In cases with weak shear, a regime characterized by the decreasing of the transversal size is found that could correspond to previous theoretical investigations. This behavior is confirmed by the analysis of the structure factor patterns.

13.
J Agric Food Chem ; 50(20): 5735-41, 2002 Sep 25.
Article in English | MEDLINE | ID: mdl-12236707

ABSTRACT

Despite extensive literature describing the biological effects of polyphenols, little is known about their absorption from diet, one major unresolved point consisting of the absorption of the bound forms of polyphenols. In this view, in the present work we studied the absorption in humans of phenolic acids from coffee, a common beverage particularly rich in bound phenolic acids, such as caffeic acid, ferulic acid, and p-coumaric acid. Coffee brew was analyzed for free and total (free + bound) phenolic acids. Chlorogenic acid (5'-caffeoylquinic acid), a bound form of caffeic acid, was present in coffee at high levels, while free phenolic acids were undetectable. After alkaline hydrolysis, which released bound phenolic acids, ferulic acid, p-coumaric acid, and high levels of caffeic acid were detected. Plasma samples were collected before and 1 and 2 h after coffee administration and analyzed for free and total phenolic acid content. Two different procedures were applied to release bound phenolic acids in plasma: beta-glucuronidase treatment and alkaline hydrolysis. Coffee administration resulted in increased total plasma caffeic acid concentration, with an absorption peak at 1 h. Caffeic acid was the only phenolic acid found in plasma samples after coffee administration, while chlorogenic acid was undetectable. Most of caffeic acid was present in plasma in bound form, mainly in the glucuronate/sulfate forms. Due to the absence of free caffeic acid in coffee, plasma caffeic acid is likely to be derived from hydrolysis of chlorogenic acid in the gastrointestinal tract.


Subject(s)
Coffee/chemistry , Hydroxybenzoates/pharmacokinetics , Absorption , Biological Availability , Blood Proteins/metabolism , Caffeic Acids/analysis , Caffeic Acids/blood , Caffeic Acids/metabolism , Chlorogenic Acid/analysis , Chlorogenic Acid/metabolism , Coumaric Acids/analysis , Glucuronidase/metabolism , Hydrogen-Ion Concentration , Hydrolysis , Hydroxybenzoates/analysis , Kinetics , Propionates
14.
Phys Rev E Stat Nonlin Soft Matter Phys ; 64(5 Pt 2): 057103, 2001 Nov.
Article in English | MEDLINE | ID: mdl-11736144

ABSTRACT

It is discussed how the equilibrium properties of the Ising model are described by an Hamiltonian with an antiferromagnetic low temperature behavior if only an heat bath dynamics, with the characteristics of a Probabilistic cellular automaton, is assumed to determine the temporal evolution of the system.

15.
Semin Nephrol ; 21(3): 262-8, 2001 May.
Article in English | MEDLINE | ID: mdl-11320491

ABSTRACT

This article starts with a concise synopsis of the history of edema. The role of underfilling, overflow, antidiuretic hormone, and acquaporins is subsequently discussed. Emphasis is given to the use of diuretics in edematous patients. The role and risks of albumin infusion are illustrated. The new hypothesis of pulse reverse osmosis is discussed. The final section deals with the measurement of colloid osmotic pressure in the clinical setting.


Subject(s)
Edema/physiopathology , Edema/therapy , Kidney Diseases/physiopathology , Kidney Diseases/therapy , Humans , Kidney/physiopathology , Nephrotic Syndrome/physiopathology , Nephrotic Syndrome/therapy
16.
Am J Kidney Dis ; 35(6): 1144-8, 2000 Jun.
Article in English | MEDLINE | ID: mdl-10845829

ABSTRACT

The study examined whether indexing glomerular filtration rate (GFR) for body surface area is appropriate for people who are severely overweight. Twenty normotensive adult men who were severely overweight but without microalbuminuria were enrolled into this study. The control group consisted of 20 healthy subjects matched for age, sex, and height. GFR was determined by measuring insulin with the continuous-infusion method. The clearance of endogenous creatinine was also measured after two daily urine collections. Renal plasma flow (RPF) was measured by p-aminohippurate clearance using the continuous-infusion method. Lean body weight was measured by impedentiometry. Adjusting for body surface area (in 1.73 m(2)) caused a significant reduction in GFR (P < 0.0001) in overweight humans (84.1 +/- 2.32 versus 109.6 +/- 3.07 mL/min/1.73 m(2)). The difference disappeared when GFR/height criteria were adopted. No difference between obese and healthy controls occurred after adjusting for lean body weight. Data for creatinine clearance paralleled those with insulin clearance; a significant reduction (P < 0.001) occurred after indexing for basal surface area, which disappeared after correction for height, as well as for lean body weight.


Subject(s)
Glomerular Filtration Rate/physiology , Obesity/physiopathology , Adipose Tissue/anatomy & histology , Adult , Albuminuria/urine , Blood Pressure/physiology , Body Constitution , Body Height , Body Mass Index , Body Surface Area , Case-Control Studies , Creatinine/urine , Electric Impedance , Follow-Up Studies , Humans , Inulin , Male , Muscle, Skeletal/anatomy & histology , Renal Plasma Flow/physiology , p-Aminohippuric Acid
17.
Miner Electrolyte Metab ; 25(1-2): 24-7, 1999.
Article in English | MEDLINE | ID: mdl-10207254

ABSTRACT

Renal reserve was explored by means of an oral protein load (2 g/kg body weight) under the form of cooked red meat in a group of 9 patients with end-stage heart failure (ESHF), class III of the New York Heart Association receiving loop diuretics and angiotensin-converting enzyme (ACE) inhibitors, and in a group of 18 healthy controls (HC) matched for age, gender, and height under an identical dietary regimen providing 40 cal/kg per day, 1 g/kg body weight of protein per day, Na 120 mmol/day, and K 50 mmol/day. Baseline glomerular filtration rate averaged 109.5+/-9.89 ml/min x 1.73 m2 in HC and 71.9+/-8.8 ml/min x 1.73 m2 in ESHF. Renal plasma flow averaged 540+/-27 ml/min x 1.73 m2 in HC and 235+/-47 ml/min x 1.73 m2 in ESHF. The filtration fraction was significantly higher in ESHF (p<0.01). Renal reserve averaged 26.03+/-3.28 ml/min x 1.73 m2 in HC and 27.2+/-7.12 ml/min x 1.73 m2 (not significant). Renal reserve averaged 123.9+/-2.9% in HC and 137.3+/-6.68% in ESHF (not significant). The filtration capacity was significantly higher in HC (p<0.001). The data point to a normalcy of renal reserve in ESHF which may depend on the chronic use of ACE inhibitors.


Subject(s)
Cardiomyopathy, Dilated/physiopathology , Kidney/physiopathology , Adult , Cardiomyopathy, Dilated/surgery , Glomerular Filtration Rate/physiology , Heart Transplantation , Humans , Male , Middle Aged , Reference Values , Renal Circulation/physiology , Waiting Lists
18.
Nephron ; 81(2): 136-40, 1999 Feb.
Article in English | MEDLINE | ID: mdl-9933747

ABSTRACT

The work was devised to compare measurements of glomerular filtration rate (GFR) by technetium-99m-diethylenetriaminepentacetic acid (99mTc-DTPA) renogram to those by creatinine clearance (measured and predicted by Cockroft and Gault) and by inulin clearance. A total number of 65 individuals were enrolled: 15 healthy controls and 50 patients with renal disease. Compared to inulin clearance used as the gold standard, 99mTc-DTPA overestimated at low and underestimated at high GFRs. 99mTc-DTPA measurements were less precise than creatinine clearance except for individuals with GFR >100 ml/min x 1.73 m2. Measured creatinine clearance had the highest correlation coefficient with inulin clearance, 99mTc-DTPA clearance the lowest. In correlation analyses, 81.5% of the interindividual variability for measured creatinine clearance could be explained by true differences in inulin clearance; this value dropped to 59.1 and 57.4% for predicted creatinine clearance and 99mTc-DTPA, respectively. In patients with GFR <25 ml/min x 1.73 m2, all 99mTc-DTPA measurements were out of the 95% confidence interval for the inulin measurement. It can be inferred that 99mTc-DTPA clearance from the renogram is less precise than measured and predicted creatinine clearance.


Subject(s)
Creatinine/metabolism , Kidney Diseases/diagnosis , Radioisotope Renography/standards , Technetium Tc 99m Pentetate , Adult , Aged , Female , Glomerular Filtration Rate , Humans , Inulin/pharmacokinetics , Kidney Glomerulus/physiology , Linear Models , Male , Metabolic Clearance Rate , Middle Aged , Radioisotope Renography/methods , Reproducibility of Results
19.
Nephron ; 76(4): 406-10, 1997.
Article in English | MEDLINE | ID: mdl-9274837

ABSTRACT

The study was devised to explore the effects of an acute oral protein load on renal hemodynamic response in patients with IgA nephropathy (IgAN). The study was performed in 10 proteinuric IgAN patients (800 +/- 300 mg/day) and in 20 healthy controls (matched by sex, age, BMI, BSA, plasma creatinine, plasma urea, urinary urea and protein intake). Blood pressure and creatinine clearance were nearly identical in the two groups. GFR and RPF, measured as the clearance of inulin and of p-aminohippurate (PAH) were studied before and after a meat meal which provided 2 g of protein/kg BW. Following the protein load, renal reserve, percent renal reserve and postmeal cumulative changes of GFR were not significantly different in IgAN and controls. Filtration fraction (FF) at baseline was significantly higher (p < 0.01) in IgAN than in controls (25.5 +/- 1.41 vs. 19 +/- 2%). Postmeal hyperemia and hyperfiltration did not affect FF in either group. Filtration capacity in IgAN was lower (p<0.02) than in controls (117 +/- 5.6 vs. 137.9 +/- 7.0 ml/min x 1.73 m2), whereas the percent of filtration capacity utilized at rest was identical in controls and in IgAN. Creatinine clearance overestimated GFR in IgAN. The data indicate that renal hemodynamic response to proteins in IgAN is normal.


Subject(s)
Dietary Proteins/pharmacology , Glomerulonephritis, IGA/physiopathology , Renal Circulation/drug effects , Adult , Female , Glomerular Filtration Rate , Humans , Kidney Function Tests , Male , Reference Values , Renal Plasma Flow/physiology
20.
Miner Electrolyte Metab ; 23(3-6): 283-6, 1997.
Article in English | MEDLINE | ID: mdl-9387134

ABSTRACT

This study was carried out to examine the renal hemodynamic response in adult patients with single kidneys born with unilateral renal agenesis. A group of 21 patients with unilateral renal agenesis were divided into three groups according to their glomerular filtration rate (GFR): 112 +/- 3 ml/min x 1.73 m2 in group A, 68 +/- 3.2 ml/min x 1.73 m2 in group B, and 40.7 +/- 3.3 ml/min x 1.73 m2 in group C. Mean arterial blood pressure was significantly higher in the patients of group C who were also proteinuric. The renal hemodynamic response to an oral protein load (2 g/kg of protein as beefsteak) was normal in all groups and unrelated to hyperfiltration or to renal failure and proteinuria. The study indicates that in patients with renal agenesis, the hemodynamic response to a protein challenge is similar to that of kidney donors, renal transplant recipients and uninephrectomized patients. The paper also demonstrates that the renal response to a protein challenge is inadequate to identify patients with renal agenesis who are at risk of developing renal disease. Finally, in renal agenesis with renal disease, creatinine clearance overestimated the GFR by an average of 32.7%.


Subject(s)
Hemodynamics/physiology , Kidney/abnormalities , Renal Insufficiency/complications , Adaptation, Physiological , Adult , Creatinine/metabolism , Female , Glomerular Filtration Rate , Humans , Insulin/metabolism , Male , Metabolic Clearance Rate , Middle Aged , Renal Insufficiency/physiopathology
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