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Bioelectrochemistry ; 59(1-2): 29-33, 2003 Apr.
Article in English | MEDLINE | ID: mdl-12699816

ABSTRACT

Immobilization of invertase in conducting copolymer matrices of 3-methylthienyl methacrylate with pyrrole and thiophene was achieved by constant potential electrolysis using sodium dodecyl sulfate (SDS) as the supporting electrolyte. Polythiophene (PTh) was also used in entrapment process for comparison. Kinetic parameters, Michaelis-Menten constant, K(m), and the maximum reaction rate, V(max), were investigated. Operational stability and temperature optimization of the enzyme electrodes were also examined.


Subject(s)
Enzymes, Immobilized/chemistry , Polymethyl Methacrylate/chemistry , Thiophenes/chemistry , beta-Fructofuranosidase/chemistry , Electrochemistry , Enzymes, Immobilized/metabolism , Kinetics , Microscopy, Electron, Scanning , Pyrroles/chemistry , Sodium Dodecyl Sulfate/chemistry , Surface Properties , Temperature , beta-Fructofuranosidase/metabolism
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