Prodiamesa olivacea Meigen and Prodiamesa bureshi Michailova (Diptera, Chironomidae, Prodiamesinae) as a candidate for assessing the genotoxicity of trace metals in fluvial sediments.

The genome response, realized by structure chromosome rearrangements in the polytene chromosomes of two sibling species Prodiamesa bureshi Michailova and Prodiamesa olivacea Mg., was studied. The larvae of the species were collected in May and September, 2016, from Biala Przemsza River, a metal-mine-affected site in southern Poland, where Zn, Cd, and Pb concentrations in the sediment exceeded many times the reference data and those from unpolluted sites. The water had high contents of different major ions and nutrients. A high spectrum of somatic chromosome aberrations was detected in the salivary gland chromosomes of both species, which defined a high somatic index (from 1.2 to 7), indicating the sensitivity of both genomes to anthropogenic stress. The cells with somatic rearrangements of both species were significantly higher (P. bureshi: G = 25.636, P < 0.001 May, G = 32.722, P < 0.001 September; P. olivacea: G = 47.863, P < 0.001 May, G = 38.742, P < 0.001 September) than the control. Both species from polluted and unpolluted sites showed a high frequency of ectopic conjugations, as between arms B, CD (centromere regions), and E (NOR). Some deformities of mentum and mandibles of P. bureshi (20%) and P. olivacea (35%) were detected. We postulate that the appearances of somatic chromosome aberrations are more sensitive indicators of genotoxicity in the studied species than changes in external morphology. The sensitivity of the P. olivacea and P. bureshi genomes shows that these species are good candidates for detecting the presence of genotoxic compounds in aquatic basins and evaluating their genotoxic effects.

A Review of Flood-Related Storage and Remobilization of Heavy Metal Pollutants in River Systems.

Recently observed rapid climate changes have focused the attention of researchers and river managers on the possible effects of increased flooding frequency on the mobilization and redistribution of historical pollutants within some river systems. This text summarizes regularities in the flood-related transport, channel-to-floodplain transfer, and storage and remobilization of heavy metals, which are the most persistent environmental pollutants in river systems. Metal-dispersal processes are essentially much more variable in alluvia than in soils of non-inundated areas due to the effects of flood-sediment sorting and the mixing of pollutants with grains of different origins in a catchment, resulting in changes of one to two orders of magnitude in metal content over distances of centimetres. Furthermore, metal remobilization can be more intensive in alluvia than in soils as a result of bank erosion, prolonged floodplain inundation associated with reducing conditions alternating with oxygen-driven processes of dry periods and frequent water-table fluctuations, which affect the distribution of metals at low-lying strata. Moreover, metal storage and remobilization are controlled by river channelization, but their influence depends on the period and extent of the engineering works. Generally, artificial structures such as groynes, dams or cut-off channels performed before pollution periods favour the entrapment of polluted sediments, whereas the floodplains of lined river channels that adjust to new, post-channelization hydraulic conditions become a permanent sink for fine polluted sediments, which accumulate solely during overbank flows. Metal mobilization in such floodplains takes place only by slow leaching, and their sediments, which accrete at a moderate rate, are the best archives of the catchment pollution with heavy metals.

Small effects of a large sediment contamination with heavy metals on aquatic organisms in the vicinity of an abandoned lead and zinc mine.

The effects of the long-term contamination of water reservoirs with mine effluents were investigated at an abandoned mine site in Upper Silesia, southern Poland. The studies covered metal content and mobility in bottom sediments as well as water chemistry in relation to the content of metals in selected macrophytes and their physiology and the composition of phyto- and zooplankton communities. Although it is 40 years since mining ceased, reservoir sediments are still heavily contaminated with cadmium, zinc and lead with concentrations (mg/kg), which vary roughly between 130­340, 10,000­50,000 and 4,000­12,000, respectively. About 50­80 % of these elements are associated with the reducible phase, and only a small percentage, <10%, is present in the most mobile exchangeable phase. Despite the high total metal concentration in sediments, their content in the submerged plants Myriophyllum spicatum and the emerged plants Phragmites australis was low. The observed effects of heavy metal contamination on photosynthetic activity in the leaves of P. australis were negligible, whereas those in M. spicatum show up only as a difference in the distribution of photosynthetic activity in leaves of different ages, which seems to be related to the very good water quality and to the generally small concentrations of metals in pond water. The physicochemical properties of water also seem to control the presence of planktonic species more than does sediment contamination. However, a shift toward groups of species known to be more resistant to heavy metals (diatoms, green algae and Rotifera) indicates some adaptative changes related to the longlasting contamination of ponds.