ABSTRACT
Actinobaculum schaalii may be a more common urinary tract pathogen than previously described. Here we report four cases of A. schaalii UTIs and we also propose a simple identification scheme to be used in the conventional microbiology laboratory based on standard biochemical tests.
Subject(s)
Actinomycetaceae/isolation & purification , Urinary Tract Infections/diagnosis , Urinary Tract Infections/drug therapy , Actinomycetaceae/drug effects , Adolescent , Adult , Aged , Aged, 80 and over , Argentina , Bacteriological Techniques , DNA, Bacterial/isolation & purification , Drug Resistance, Multiple, Bacterial , Humans , Male , Middle Aged , RNA, Ribosomal, 16S/isolation & purification , Sequence Analysis, DNA , Urinary Tract Infections/microbiology , Young AdultSubject(s)
Disease Outbreaks , Pseudomonas Infections/epidemiology , Pseudomonas Infections/microbiology , Pseudomonas aeruginosa/classification , Pseudomonas aeruginosa/isolation & purification , Anti-Bacterial Agents/pharmacology , Argentina/epidemiology , Bacterial Proteins/genetics , Carbapenems/pharmacology , Humans , Microbial Sensitivity Tests , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/genetics , beta-Lactam Resistance , beta-Lactamases/geneticsABSTRACT
Bifidobacterium species are difficult to identify and may be underreported or not recovered by many laboratories because of their slow growth. We emphasize the importance of the Gram stain in urine samples and the addition of enriched media and enhanced atmosphere over time for urine cultures with pyuria. This is the first report of a Bifidobacterium scardovii recurrent urinary infection in an elderly woman.
Subject(s)
Bifidobacteriales Infections/diagnosis , Bifidobacteriales Infections/pathology , Bifidobacterium/classification , Bifidobacterium/isolation & purification , Aged, 80 and over , Bacterial Typing Techniques , Bacteriological Techniques/methods , Bifidobacteriales Infections/microbiology , Bifidobacterium/genetics , Cluster Analysis , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Female , Gentian Violet , Humans , Molecular Sequence Data , Phenazines , Phylogeny , Pyuria/diagnosis , Pyuria/microbiology , Pyuria/pathology , RNA, Ribosomal, 16S/genetics , Recurrence , Sequence Analysis, DNA , Urine/microbiologyABSTRACT
Non-enterococcal Gram-positive bacteria that are intrinsically vancomycin-resistant have been infrequently isolated in association with serious infections. However, well-documented infections have lately been reported with increasing frequency. Because these organisms may be pathogens, we tested the MICs of 19 antimicrobial agents by the agar dilution method for predicting susceptibility. The activity of these antimicrobial agents was assessed against 28 strains (Lactobacillus rhamnosus, 6; Lactobacillus acidophilus, 1; Lactobacillus casei, 1; Lactobacillus fermentum, 2; Lactobacillus brevis, 1; Lactobacillus plantarum, 1; Weissella confusa, 2; Leuconostoc mesenteroides, 7; Leuconostoc lactis, 4; Pediococcus acidilactici, 2; Pediococcus pentosaceus, 1), isolated from clinical specimens in an Argentinian university hospital from 1997 to 2003. The MICs of penicillin for 67% of the Lactobacillus strains and 100% of the Leuconostoc spp. and Pediococcus spp. strains tested were in the 0.25-2 microg/mL range. Erythromycin was the most active antimicrobial overall. Multiresistance was observed in 2 strains (Lactobacillus rhamnosus, 1; Lactobacillus plantarum, 1).
