ABSTRACT
En nuestro servicio se realizaron entre 1987 y 1997, 115 nefrectomías por carcinoma renal, de las cuales 7 (6 por ciento) presentaban la variedad histológica sarcomatoide. En 6 casos, el tumor había sobrepasado la cápsula renal, en el restante estaba confinado al parénquima renal; éste fue uno de los cuales en los que no se observó progresión de la enfermedad luego del tratamiento quirúrgico. Llevamos a cabo estudios de morfología celular con hematoxilina-eosina, inmunohistoquímica (MIB) y ploidía celular por medio de citometría de flujo, comparando en forma retrospectiva la evolución clínica de este tip de tumor y la utilidad de los métodos empleados como factores pronósticos de progresión de la enfermedad
Subject(s)
Humans , Male , Female , Flow Cytometry , Kidney Neoplasms/classification , Kidney Neoplasms/surgery , Ploidies , PrognosisABSTRACT
En nuestro servicio se realizaron entre 1987 y 1997, 115 nefrectomías por carcinoma renal, de las cuales 7 (6 por ciento) presentaban la variedad histológica sarcomatoide. En 6 casos, el tumor había sobrepasado la cápsula renal, en el restante estaba confinado al parénquima renal; éste fue uno de los cuales en los que no se observó progresión de la enfermedad luego del tratamiento quirúrgico. Llevamos a cabo estudios de morfología celular con hematoxilina-eosina, inmunohistoquímica (MIB) y ploidía celular por medio de citometría de flujo, comparando en forma retrospectiva la evolución clínica de este tip de tumor y la utilidad de los métodos empleados como factores pronósticos de progresión de la enfermedad(AU)
Subject(s)
Humans , Male , Female , Kidney Neoplasms/classification , Kidney Neoplasms/surgery , Ploidies , Flow Cytometry , PrognosisABSTRACT
Extremely halophilic archaea, which flourish in hypersaline environments, are known to contain a variety of large dynamic replicons. Previously, the analysis of one such replicon, pNRC100, in Halobacterium sp. strain NRC-1, showed that it undergoes high-frequency insertion sequence (IS) element-mediated insertions and deletions, as well as inversions via recombination between 39-kb-long inverted repeats (IRs). Now, the complete sequencing of pNRC100, a 191,346-bp circle, has shown the presence of 27 IS elements representing eight families. A total of 176 ORFs or likely genes of 850-bp average size were found, 39 of which were repeated within the large IRs. More than one-half of the ORFs are likely to represent novel genes that have no known homologs in the databases. Among ORFs with previously characterized homologs, three different copies of putative plasmid replication and four copies of partitioning genes were found, suggesting that pNRC100 evolved from IS element-mediated fusions of several smaller plasmids. Consistent with this idea, putative genes typically found on plasmids, including those encoding a restriction-modification system and arsenic resistance, as well as buoyant gas-filled vesicles and a two-component regulatory system, were found on pNRC100. However, additional putative genes not expected on an extrachromosomal element, such as those encoding an electron transport chain cytochrome d oxidase, DNA nucleotide synthesis enzymes thioredoxin and thioredoxin reductase, and eukaryotic-like TATA-binding protein transcription factors and a chromosomal replication initiator protein were also found. A multi-step IS element-mediated process is proposed to account for the acquisition of these chromosomal genes. The finding of essential genes on pNRC100 and its property of resistance to curing suggest that this replicon may be evolving into a new chromosome.
Subject(s)
Halobacterium/genetics , Replicon , Chromosome Mapping , Chromosomes, Archaeal/genetics , DNA Transposable Elements/genetics , DNA, Archaeal/chemistry , DNA, Archaeal/genetics , Gene Library , Genes, Archaeal/genetics , Molecular Sequence Data , Open Reading Frames/genetics , Plasmids/genetics , Sequence Analysis, DNAABSTRACT
We have developed a chicken (Gallus domesticus) Z-chromosome-specific DNA library in a phage vector by means of chromosome microisolation and microcloning. The chromosomal origin, specificity, and purity was evaluated by fluorescent in situ hybridization (FISH) on chicken metaphases. Heterologous chromosome painting using this Z-chromosome-specific probe on turkey (Meleagris gallopavo) metaphases identified its homologous Z-chromosome, under the same stringent conditions as that used in the chicken, indicating a high degree of Z-chromosome sequence homology among these two species. This chicken Z-chromosome library will facilitate the development of Z-chromosome-specific DNA markers that will be useful for genetic mapping in the domestic chicken and related avian species. The Z-chromosome-specific DNA probe will also be useful for studies pertaining to the sex chromosome evolution in avian species.
