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1.
Phytochem Anal ; 28(6): 550-557, 2017 Nov.
Article in English | MEDLINE | ID: mdl-28670816

ABSTRACT

INTRODUCTION: Hieracium pilosella L. is a medicinal plant encountered in Europe in traditional herbal medicinal products. Caffeoylquinic (ortho-dihydroxycinnamic) acid derivatives are characteristic constituents used as analytical markers in the quality control of the herbal material. Until now, the caffeoylquinic acid derivatives have been assayed using a colorimetric method according to the French Pharmacopoeia. OBJECTIVE: To develop an HPLC-method for quantification of caffeoylquinic acid derivatives in H. pilosella. METHODOLOGY: Samples were prepared by methanol extraction of H. pilosella, dried herb. An HPLC method suitable for analysis was developed and validated. The content of caffeoylquinic acid derivatives was determined and expressed as chlorogenic acid. Individual substances in the samples were identified by analyses of UV-MS/MS spectra and by comparisons with spectra and chromatographic retention times of authentic reference substances. RESULTS: Validation showed that the chromatographic method has good selectivity with no interfering peaks. Sensitivity, linearity, repeatability and accuracy were shown to be adequate. In analyses of several batches of H. pilosella, contents of caffeoylquinic acids ranging from 0.7 to 1.9% were found. Compared to the colorimetric method, this newly developed HPLC method is more specific and results in detection of nominally lower amounts of caffeoylquinic acid derivatives. A new acceptance limit of 1.0% instead of 2.5% caffeoylquinic acid, expressed as chlorogenic acid, for H. pilosella, is proposed when using this HPLC-method. CONCLUSION: A newly developed HPLC method is shown to be appropriate for quantitative determination of caffeoylquinic acid derivatives in H. pilosella. Copyright © 2017 John Wiley & Sons, Ltd.


Subject(s)
Asteraceae/chemistry , Chromatography, High Pressure Liquid/methods , Quinic Acid/analogs & derivatives , Molecular Structure , Quinic Acid/chemistry , Reproducibility of Results , Sensitivity and Specificity
2.
J Ethnopharmacol ; 158 Pt B: 463-6, 2014 Dec 02.
Article in English | MEDLINE | ID: mdl-25043782

ABSTRACT

Based on the regulatory requirements on efficacy documentation in the European Union, the herbal medicinal products have been grouped into the following sections: (i) Herbal medicinal products for which the efficacy is demonstrated by results of a "full" set of clinical trials that are in conformity with the relevant guidelines of the therapeutic area in question. This regulatory pathway to obtain a marketing authorisation for a new medicinal product (new chemical entity) is open to herbal medicinal products, but the examples are in reality few. (ii) Herbal medicinal products which have a "well-established medicinal use with a recognised efficacy and an acceptable level of safety" in the European Union. Results of new and product specific clinical trials are not required to obtain a marketing authorisation for products that fulfil these criteria, but a substantial clinical experience must be documented and sufficient scientific data on efficacy must be publicly available. (iii) "Traditional" herbal medicinal products, that do not fulfil the efficacy requirements for a marketing authorisation, but for which a medicinal use of at least 30 years including 15 years in the European Union can be documented. Traditional herbal medicinal products can only be registered with therapeutic indications that are considered safe for use without the supervision of a physician. After briefly reviewing the regulatory requirements on efficacy documentation of herbal medicinal products in the European Union, some concluding remarks on the past and future developments in the area are made.


Subject(s)
Legislation, Drug , Medicine, Traditional/standards , Plant Preparations/standards , Drug Approval/legislation & jurisprudence , European Union , Humans , Phytotherapy/standards , Time Factors
3.
Biopolymers ; 94(5): 626-34, 2010.
Article in English | MEDLINE | ID: mdl-20564012

ABSTRACT

Cycloviolacin O2 is a small cyclic cysteine-rich protein belonging to the group of plant proteins called cyclotides. This cyclotide has been previously shown to exert cytotoxic activity against a variety of human tumor cell lines as well as primary cultures of human tumor cells in vitro. This study is the first evaluation of its tolerability and antitumor activity in vivo. Maximal-tolerated doses were estimated to 1.5 mg/kg for single intravenous (i.v.) dosing and 0.5 mg/kg for daily repeated dosing, respectively. Two different in vivo methods were used: the hollow fiber method with single dosing (i.v., 1.0 mg/kg) and traditional xenografts with repeated dosing over 2 weeks (i.v., 0.5 mg/kg daily, 5 days a week). The human tumor cell lines used displayed dose-dependent in vitro sensitivity (including growth in hollow fibers to confirm passage of cycloviolacin O2 through the polyvinylidene fluoride fibers), with IC5o values in the micromolar range. Despite this sensitivity in vitro, no significant antitumor effects were detected in vivo, neither with single dosing in the hollow fiber method nor with repeated dosing in xenografts. In summary, the results indicate that antitumor effects are minor or absent at tolerable (sublethal) doses, and cycloviolacin O2 has a very abrupt in vivo toxicity profile, with lethality after single injection at 2 mg/kg, but no signs of discomfort to the animals at 1.5 mg/kg. Repeated dosing of 1 mg/kg gave a local-inflammatory reaction at the site of injection after 2-3 days; lower doses were without complications.


Subject(s)
Antineoplastic Agents/pharmacology , Cell Line, Tumor/drug effects , Cyclotides/pharmacology , Plant Proteins/pharmacology , Amino Acid Sequence , Animals , Antineoplastic Agents/administration & dosage , Cyclotides/administration & dosage , Cyclotides/genetics , Dose-Response Relationship, Drug , Drug Delivery Systems , Female , Humans , Male , Mice , Mice, Nude , Molecular Sequence Data , Plant Proteins/administration & dosage , Plant Proteins/genetics , Protein Structure, Tertiary , Transplantation, Heterologous
4.
J Biol Chem ; 284(16): 10672-83, 2009 Apr 17.
Article in English | MEDLINE | ID: mdl-19211551

ABSTRACT

Cyclotides are a family of plant defense proteins that are highly resistant to adverse chemical, thermal, and enzymatic treatment. Here, we present the first crystal structure of a cyclotide, varv F, from the European field pansy, Viola arvensis, determined at a resolution of 1.8 A. The solution state NMR structure was also determined and, combined with measurements of biophysical parameters for several cyclotides, provided an insight into the structural features that account for the remarkable stability of the cyclotide family. The x-ray data confirm the cystine knot topology and the circular backbone, and delineate a conserved network of hydrogen bonds that contribute to the stability of the cyclotide fold. The structural role of a highly conserved Glu residue that has been shown to regulate cyclotide function was also determined, verifying its involvement in a stabilizing hydrogen bond network. We also demonstrate that varv F binds to dodecylphosphocholine micelles, defining the binding orientation and showing that its structure remains unchanged upon binding, further demonstrating that the cyclotide fold is rigid. This study provides a biological insight into the mechanism by which cyclotides maintain their native activity in the unfavorable environment of predator insect guts. It also provides a structural basis for explaining how a cluster of residues important for bioactivity may be involved in self-association interactions in membranes. As well as being important for their bioactivity, the structural rigidity of cyclotides makes them very suitable as a stable template for peptide-based drug design.


Subject(s)
Cyclotides , Cystine Knot Motifs , Drug Design , Insecticides , Protein Conformation , Protein Folding , Amino Acid Sequence , Animals , Crystallography, X-Ray , Cyclotides/chemistry , Cyclotides/genetics , Cyclotides/metabolism , Humans , Hydrogen Bonding , Insecticides/chemistry , Insecticides/metabolism , Molecular Sequence Data , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Sequence Alignment
5.
Curr Protein Pept Sci ; 5(5): 317-29, 2004 Oct.
Article in English | MEDLINE | ID: mdl-15544528

ABSTRACT

This review focuses on the discovery of cyclotides in the Violaceae, their isolation and their anti-cancer effects. These macrocyclic plant peptides consist of about 30 amino acids, including three conserved disulfide bonds in a cystine knotted arrangement, which renders them a remarkable stability. Their unique structure, combined with a wide array of biological activities, makes them of great interest as possible leads in drug development or as carriers of grafted peptide sequences. Here we describe the work conducted in our laboratory, which started with the overall aim of identifying peptides and small proteins of the size 10-50 amino acid residues in plants with novel chemical structures and biological profiles with a potential for drug development or for use as pharmacological tools. Thus we developed a fractionation protocol to directly address major challenges encountered when dealing with plant material, such as removal of chlorophyll, polyphenols, and low molecular compounds omnipresent in plants. Using this protocol, we then discovered a suite of cyclotides, the varv peptides, from the plant Viola arvensis (Violaceae). Following this, separation methods directly targeting cyclotides were developed, e.g. by adsorption, ion exchange chromatography and solvent-solvent partitioning, which then were used in the isolation of additional cyclotides. To structurally examine cyclotides we have also developed methods based on mass spectrometry for cyclotide sequencing and mapping of disulfide bonds. Finally, to assess structure-activity relationships, regarding their anti-cancer and cytotoxic effects that we focus upon, we have also characterized the three dimensional structure of cyclotides by homology modeling techniques.


Subject(s)
Cyclotides/chemistry , Cyclotides/isolation & purification , Violaceae/chemistry , Animals , Antineoplastic Agents/chemistry , Antineoplastic Agents/isolation & purification , Antineoplastic Agents/metabolism , Antineoplastic Agents/pharmacology , Chromatography, Liquid , Cyclotides/metabolism , Cyclotides/pharmacology , Disulfides/metabolism , Humans , Structure-Activity Relationship
6.
J Nat Prod ; 67(8): 1287-90, 2004 Aug.
Article in English | MEDLINE | ID: mdl-15332843

ABSTRACT

Cycloviolacin O2, a plant peptide of the cyclotide family, is shown to have potent effects against fouling barnacles (Balanus improvisus), with complete inhibition of settlement at a concentration of 0.25 microM. The effect of cycloviolacin O2 against barnacles is reversible and nontoxic in the bioassay employed in these studies. Cycloviolacin O2 was isolated from the terrestrial plant Viola odorata by strong cation exchange and reversed-phase HPLC and identified by mass spectrometry following aminoethylation and enzymatic cleavage.


Subject(s)
Cyclotides , Marine Biology , Peptides, Cyclic/pharmacology , Thoracica/drug effects , Viola/chemistry , Amino Acid Sequence , Animals , Chromatography, High Pressure Liquid , Molecular Sequence Data , Seawater/chemistry , Structure-Activity Relationship
7.
J Nat Prod ; 67(5): 806-10, 2004 May.
Article in English | MEDLINE | ID: mdl-15165141

ABSTRACT

Cyclotides, a family of approximately 50 mini-proteins isolated from various Violaceae and Rubiaceae plants, are characterized by their circular peptide backbone and six conserved cysteine residues arranged in a cystine knot motif. Cyclotides show a wide range of biological activities, making them interesting targets for both pharmaceutical and agrochemical research, but little is known about their natural function and the events that trigger their expression. An investigation of the geographical and seasonal variations of cyclotide profiles has been performed, using the native Australian violet, Viola hederacea, and the Swedish sweet violet, Viola odorata, as model plants. The results showed that in the Australian violet the relative peptide levels of some cyclotides remained almost constant throughout the year, while other cyclotides were present only at certain times of the year. Therefore, it appears that V. hederacea expresses a basic armory of cyclotides as well as special "add-ons" whose levels are influenced by external factors. In the Swedish violet, cyclotide levels were increased up to 14 times during the warmest period of the year. The larger variation in expression levels of the Swedish plants may be a reflection of a greater climatic variation.


Subject(s)
Cyclotides , Genetic Variation , Peptides, Cyclic/chemistry , Peptides, Cyclic/genetics , Plant Proteins/chemistry , Plant Proteins/genetics , Viola/chemistry , Amino Acid Sequence , Climate , Databases, Protein , Models, Molecular , Molecular Sequence Data , Sequence Alignment , Sequence Analysis, Protein , Sequence Homology, Amino Acid , Sweden
8.
J Nat Prod ; 67(2): 144-7, 2004 Feb.
Article in English | MEDLINE | ID: mdl-14987049

ABSTRACT

A crude fraction of Viola tricolor rich in small lipophilic proteins was prepared and subjected to fractionation guided by bioactivity, using RP-HPLC and a fluorometric cytotoxicity assay. Two human cancer cell lines, U-937 GTB (lymphoma) and RPMI-8226/s (myeloma), were used in this study. The most potent compounds isolated, that is, the compounds showing the lowest IC(50) values, were shown to be three small proteins: vitri A (IC(50) = 0.6 microM and IC(50) = 1 microM, respectively), varv A (IC(50) = 6 microM and IC(50) = 3 microM, respectively), and varv E (IC(50) = 4 microM in both cell lines). Their sequences, determined by automated Edman degradation, quantitative amino acid analysis, and mass spectrometry, were cyclo-GESCVWIPCITSAIGCSCKSKVCYRNGIPC (vitri A), cyclo-GETCVGGTCNTPGCSCSWPVCTRNGLPVC (varv A), and cyclo-GETCVGGTCNTPGCSCSWPVCTRNGLPIC (varv E), of which vitri A is described for the first time. Each forms a head-to-tail cyclic backbone, with six cysteine residues being involved in three disulfide bonds, characteristic of the family of small proteins called the cyclotides. This is the first report on cyclotides from the species V. tricolor and the first report on the sequence of the cytotoxic cyclotide vitri A.


Subject(s)
Peptides, Cyclic/chemistry , Plant Proteins/chemistry , Plants, Medicinal/chemistry , Viola/chemistry , Amino Acid Sequence , Amino Acids/analysis , Cell Line, Tumor , Drug Screening Assays, Antitumor , Germany , Humans , Inhibitory Concentration 50 , Molecular Sequence Data , Structure-Activity Relationship
9.
Phytochemistry ; 64(1): 135-42, 2003 Sep.
Article in English | MEDLINE | ID: mdl-12946412

ABSTRACT

Two polypeptides named vodo M and vodo N, both of 29 amino acids, have been isolated from Viola odorata L. (Violaceae) using ion exchange chromatography and reversed phase HPLC. The sequences were determined by automated Edman degradation, quantitative amino acid analysis, and mass spectrometry (MS). Using MS, it was established that vodo M (cyclo-SWPVCTRNGAPICGESCFTGKCYTVQCSC) and vodo N (cyclo-SWPVCYRNGLPVCGETCTLGKCYTAGCSC) form a head-to-tail cyclic backbone and that six cysteine residues are involved in three disulphide bonds. Their origin, sequences, and cyclic nature suggest that these peptides belong to the family of cyclic plant peptides, called cyclotides. The three-dimensional structures of vodo M and vodo N were modelled by homology, using the experimentally determined structure of the cyclotide kalata B1 as the template. The images of vodo M and vodo N show amphipathic structures with considerable surface hydrophobicity for a protein modelled in a polar environment.


Subject(s)
Cyclotides , Peptides, Cyclic/chemistry , Peptides, Cyclic/genetics , Viola/chemistry , Amino Acid Sequence , Computer Simulation , Databases, Protein , Models, Molecular , Molecular Sequence Data , Peptides, Cyclic/isolation & purification , Protein Conformation , Rubiaceae/chemistry , Sequence Alignment , Sequence Analysis, Protein , Sequence Homology, Amino Acid , Structural Homology, Protein
10.
Anal Biochem ; 318(1): 107-17, 2003 Jul 01.
Article in English | MEDLINE | ID: mdl-12782038

ABSTRACT

The expression of cyclotides-macrocyclic plant peptides-was profiled in six violets, Viola cotyledon, V. biflora, V. arvensis, V. tricolor, V. riviniana, and V. odorata, by LC-MS. All were found to express notably complex mixtures, with single species containing >50 cyclotides. To facilitate their sequencing by MS-MS, an analytical strategy is presented involving aminoethylation of cysteines. This overcomes a number of problems intimately associated with the cyclotide core structure-that is, their joined N and C termini, disulfide knot, and low or clustered content of positively charged amino acids and enzymatic cleavage sites. As a result, charges as well as cleavage sites are introduced at the most conserved part of their sequence, the cysteines. Combined with tryptic digestion, all intercysteine loops are then of suitable size and charge for MS-MS sequencing. The utility of this strategy is shown by the sequencing of two novel cyclotides isolated from V. cotyledon; vico A (cyclo-(AESCVYIPCFTGIAGCSCKNKVCYYNGSIPC)) and vico B (cyclo-(AESCVYIPCITGIAGCSCKNKVCYYNGSIPC)); their complete sequence could be determined by nanospray MS-MS. The strategy for converting conserved cysteines to enzymatic cleavage sites might also benefit the study of other peptides and proteins displaying similar structural problems for MS analysis.


Subject(s)
Cysteine/analogs & derivatives , Cysteine/chemistry , Peptides, Cyclic/metabolism , Plant Proteins/metabolism , Viola/metabolism , Amino Acid Sequence , Amino Acids/analysis , Chromatography, Liquid , Mass Spectrometry/methods , Molecular Sequence Data , Molecular Weight , Peptides, Cyclic/analysis , Peptides, Cyclic/chemistry , Plant Proteins/analysis , Plant Proteins/chemistry , Viola/chemistry
11.
Phytochemistry ; 63(3): 249-55, 2003 Jun.
Article in English | MEDLINE | ID: mdl-12737975

ABSTRACT

Through a reliable and repeatable procedure based on solid-phase extraction techniques, a protein fraction (P fraction) rich in Cys/Gly residues was extracted and captured from oat (Avena sativa L.) seeds. Quantitative amino acid analysis and MS of the P fraction indicated that it contains a series of heterogeneous Cys/Gly-rich proteins with molecular masses of 3.6-4.0 kDa. Preliminary results from bioassays showed that these proteins possess weak to moderate antifungal properties to some fungal strains. From this fraction, a new polypeptide, designated avesin A, was purified and sequenced by Edman degradation. Avesin A consists of 37 amino-acid residues, with 10 glycine residues and eight cysteine residues forming disulfide bridges, and contains a single chitin-binding domain, which indicates that avesin A is a new member of the putative chitin-binding proteins. Avesin A is the first identified hevein-like small protein from cereal grains.


Subject(s)
Avena/chemistry , Chitin/metabolism , Plant Proteins/chemistry , Seeds/chemistry , Amino Acid Sequence , Amino Acids/analysis , Antifungal Agents/chemistry , Cysteine/chemistry , Disulfides/analysis , Disulfides/chemistry , Glycine/chemistry , Molecular Sequence Data , Molecular Weight , Plant Proteins/genetics , Plant Proteins/isolation & purification , Plant Proteins/pharmacology , Protein Structure, Tertiary , Sequence Homology, Amino Acid , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
12.
Mol Cancer Ther ; 1(6): 365-9, 2002 Apr.
Article in English | MEDLINE | ID: mdl-12477048

ABSTRACT

Cytotoxic activities of three naturally occurring macrocyclic peptides (cyclotides) isolated from the two violets, Viola arvensis Murr. and Viola odorata L., were investigated. A nonclonogenic fluorometric microculture assay was used to examine cytotoxicity in a panel of 10 human tumor cell lines representing defined types of cytotoxic drug resistance. Additionally, primary cultures of tumor cells from patients, and for comparison normal lymphocytes, were used to quantify cytotoxic activity. All three cyclotides, varv A, varv F, and cycloviolacin 02, exhibited strong cytotoxic activities, which varied in a dose-dependent manner. Cycloviolacin 02 was the most potent in all cell lines (IC50 0.1-0.3 microM), followed by varv A (IC50 2.7-6.35 microM) and varv F (IC50 2.6-7.4 microM), respectively. Activity profiles of the cyclotides differed significantly from those of antitumor drugs in clinical use, which may indicate a new mode of action. This, together with the exceptional chemical and biological stability of cyclotides, makes them interesting in particular for their potential as pharmacological tools and possibly as leads to antitumor agents.


Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Cyclotides , Neoplasms/drug therapy , Peptides, Cyclic/pharmacology , Amino Acid Sequence , Cell Division/drug effects , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , Humans , Molecular Sequence Data , Peptides, Cyclic/isolation & purification , Tumor Cells, Cultured , Viola/chemistry
13.
J Biomol Screen ; 7(4): 333-40, 2002 Aug.
Article in English | MEDLINE | ID: mdl-12230887

ABSTRACT

Chosen to reflect biodiversity in a phylogenetic sense, 100 fractionated plant extracts were screened in vitro for cytotoxicity following extraction and fractionation (polypeptide isolation). Of these 100 extracts, 30 were selected and then characterized preliminarily for antitumor potency and mode of action by testing them on two cell lines and primary cultures of human tumor cells. On the basis of cytotoxicity potency, 10 of the extracts were further characterized for anticancer activity in 10 human tumor cell lines. This final testing resulted in seven potential lead plants with superior evidence of antitumor potential: Colchicum autumnale L. (Colchicaceae), Digitalis lanata Ehrh. and Digitalis purpurea L. (Plantaginaceae), Helleborus cyclophyllus Boiss. (Ranunculaceae), Menyanthes trifoliata L. (Menyanthaceae), and Viola arvensis Murr. and Viola patrinii Ging. (Violaceae). Within a database of antitumor compounds, the activity profiles of the extracts from these seven plants were compared, by correlation analysis, with those of more than 100 other compounds, including 39 standard drugs from different classes of cytotoxic mechanisms. The activity profiles of six of these candidates were uncorrelated with those of the standard drugs, possibly indicating new pathways of drug-mediated cell death.


Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Drug Screening Assays, Antitumor , Plants, Medicinal/chemistry , Automation , Cell Division/drug effects , Dose-Response Relationship, Drug , Evaluation Studies as Topic , Humans , Plant Extracts/pharmacology , Quality Control , Tumor Cells, Cultured
14.
Biochem J ; 366(Pt 2): 405-13, 2002 Sep 01.
Article in English | MEDLINE | ID: mdl-12049612

ABSTRACT

A new basic protein, designated ligatoxin B, containing 46 amino acid residues has been isolated from the mistletoe Phoradendron liga (Gill.) Eichl. (Viscaceae). The protein's primary structure, determined unambiguously using a combination of automated Edman degradation, trypsin enzymic digestion, and tandem MS analysis, was 1-KSCCPSTTAR-NIYNTCRLTG-ASRSVCASLS-GCKIISGSTC-DSGWNH-46. Ligatoxin B exhibited in vitro cytotoxic activities on the human lymphoma cell line U-937-GTB and the primary multidrug-resistant renal adenocarcinoma cell line ACHN, with IC50 values of 1.8 microM and 3.2 microM respectively. Sequence alignment with other thionins identified a new member of the class 3 thionins, ligatoxin B, which is similar to the earlier described ligatoxin A. As predicted by the method of homology modelling, ligatoxin B shares a three-dimensional structure with the viscotoxins and purothionins and so may have the same mode of cytotoxic action. The novel similarities observed by structural comparison of the helix-turn-helix (HTH) motifs of the thionins, including ligatoxin B, and the HTH DNA-binding proteins, led us to propose the working hypothesis that thionins represent a new group of DNA-binding proteins. This working hypothesis could be useful in further dissecting the molecular mechanisms of thionin cytotoxicity and of thionin opposition to multidrug resistance, and useful in clarifying the physiological function of thionins in plants.


Subject(s)
Cell Survival/drug effects , DNA-Binding Proteins/isolation & purification , Mistletoe/chemistry , Plant Proteins/isolation & purification , Amino Acid Sequence , Binding Sites , Chromatography, High Pressure Liquid , Cytotoxins/chemistry , Cytotoxins/isolation & purification , Cytotoxins/toxicity , DNA/chemistry , DNA/metabolism , DNA-Binding Proteins/chemistry , DNA-Binding Proteins/toxicity , Helix-Turn-Helix Motifs , Humans , Lymphoma , Mass Spectrometry , Models, Molecular , Molecular Sequence Data , Peptide Fragments/chemistry , Plant Proteins/chemistry , Plant Proteins/toxicity , Sequence Alignment , Sequence Homology, Amino Acid , Tumor Cells, Cultured
15.
J Nat Prod ; 65(1): 32-41, 2002 Jan.
Article in English | MEDLINE | ID: mdl-11809061

ABSTRACT

A multitarget functional bioassay was optimized as a method for detecting substances interacting with the inflammatory process of activated neutrophil granulocytes, mainly to release elastase detected by p-nitroanilide (pNA) formation. Using this bioassay, 100 fractionated extracts of 96 plants were screened, with results presented in a manner that links recorded biological activity to phylogenetic information. The plants were selected to represent a major part of the angiosperms, with emphasis on medicinal plants, Swedish anti-inflammatory plants, and plants known to contain peptides. Of the tested extracts, 41% inhibited pNA formation more than 60%, and 3% stimulated formation. The extract of Digitalis purpurea enhanced pNA formation, and digitoxin, the active compound, was isolated and identified. Plant extracts that exhibited potent nonselective inhibition (>80% inhibition) were evaluated further for direct inhibition of isolated elastase and trypsin enzyme. The inhibitory effect of most tested extracts on the isolated enzyme elastase was similar to that of PAF- and fMLP-induced pNA formation. Compared to trypsin, inhibition of elastase by extracts of Rubus idaeus and Tabernaemontana dichotoma was significantly higher (80% and 99%, respectively). Inhibition of trypsin by the extract of Reseda luteola was high (97%). Orders such as Lamiales and Brassicales were shown to include a comparably high proportion of plants with inhibitory extracts.


Subject(s)
Anti-Inflammatory Agents/pharmacology , Biological Assay/methods , Biological Products/pharmacology , Leukocyte Elastase/analysis , Magnoliopsida/chemistry , Neutrophil Activation , Neutrophils , Plants, Medicinal/chemistry , Plants, Medicinal/classification , Anti-Inflammatory Agents/chemistry , Anti-Inflammatory Agents/isolation & purification , Benzoylarginine Nitroanilide/analysis , Biological Products/chemistry , Biological Products/isolation & purification , Cytochalasin B , Digitalis/chemistry , Digitonin/chemistry , Digitonin/pharmacology , Digitoxin/chemistry , Digitoxin/isolation & purification , Digitoxin/pharmacology , Dose-Response Relationship, Immunologic , Hemolysis , Humans , Lamiaceae/chemistry , Leukocyte Elastase/antagonists & inhibitors , Leukocyte Elastase/metabolism , N-Formylmethionine Leucyl-Phenylalanine , Neutrophil Activation/drug effects , Neutrophils/drug effects , Neutrophils/enzymology , Peptides , Plant Proteins/analysis , Plant Proteins/chemistry , Plant Proteins/pharmacology , Platelet Activating Factor , Superoxides/pharmacology , Sweden , Tabernaemontana/chemistry , Trypsin Inhibitors , Violaceae/chemistry , alpha-Amylases/antagonists & inhibitors
16.
Phytochemistry ; 59(2): 169-73, 2002 Jan.
Article in English | MEDLINE | ID: mdl-11809452

ABSTRACT

The cyclohexenyl chalcone derivative [(-)-hydroxypanduratin A], together with the previously known panduratin A, sakuranetin, pinostrobin, pinocembrin, and dihydro-5,6-dehydrokawain were isolated from the chloroform extract of the red rhizome variety of Boesenbergia pandurata (Robx.) Schltr. [currently known as Boesenbergia rotunda (L.) Mansf., Kulturpfl.]. Their structures were assigned on the basis of their spectroscopic data. (-)-Hydroxypanduratin A and (-)-panduratin A showed significant topical anti-inflammatory activity in the assay of TPA-induced ear edema in rats.


Subject(s)
Anti-Inflammatory Agents/therapeutic use , Cyclohexanes/therapeutic use , Edema/drug therapy , Zingiberaceae/chemistry , Animals , Anti-Inflammatory Agents/chemistry , Anti-Inflammatory Agents/isolation & purification , Chalcone/analogs & derivatives , Chalcone/chemistry , Chalcone/isolation & purification , Chalcone/therapeutic use , Cyclohexanes/chemistry , Ear/pathology , Edema/chemically induced , Rats , Tetradecanoylphorbol Acetate/antagonists & inhibitors
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