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1.
Emotion ; 2024 Jun 03.
Article in English | MEDLINE | ID: mdl-38829352

ABSTRACT

Existing emotion regulation research focuses on how individuals use different strategies to manage their own emotions-also called intra-personal emotion regulation. However, people often leverage connections with others to regulate their own emotions-interpersonal emotion regulation. The goal of the present studies was to develop a comprehensive and efficient scale-the Emotion Regulation Strategies Scale (ERSS)-to assess nine specific emotion regulation strategies that individuals use both intra-personally and interpersonally. These emotion regulation strategies were cognitive reappraisal, distraction, situation selection, problem solving, acceptance, calming, savoring, rumination, and expressive suppression. Data were collected between 2020 and 2022. Study 1 adopted a qualitative approach to establish original scale items. Results of the confirmatory factor analysis in Study 2 confirmed a nine-factor solution for both the intra- and the interpersonal scales and finalized scale items. A second confirmatory factor analysis in Study 3 found the ERSS for both the intra-personal and interpersonal scale models to possess good model fit. Correlations from Study 3 showed the ERSS subscales to be related in expected ways to existing emotion regulation scales, yet not redundant with these scales. The degree to which individuals used the range of intra- and interpersonal emotion regulation strategies assessed on the ERSS also related to the levels of clinical symptoms. The ERSS represents a comprehensive novel scale that can flexibly assess a range of specific emotion regulation strategies used both intra- and interpersonally. Future work should be conducted using the ERSS cross culturally and in clinical samples. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

2.
Community Health Equity Res Policy ; 44(3): 265-279, 2024 Apr.
Article in English | MEDLINE | ID: mdl-37202859

ABSTRACT

A qualitative, community-engaged assessment was conducted to identify needs and priorities for infant obesity prevention programs among mothers participating in home visiting programs. Thirty-two stakeholders (i.e., community partners, mothers, home visitors) affiliated with a home visiting program serving low-income families during the prenatal to age three period participated in group level assessment sessions or individual qualitative interviews. Results indicated families face many challenges to obesity prevention particularly in terms of healthy eating. An obesity prevention program can address these challenges by offering realistic feeding options and non-judgmental peer support, improving access to resources, and tailoring program content to individual family needs and preferences. Informational needs, family factors in healthy eating outcomes, and the importance of access and awareness of programs were also noted. To ensure the cultural- and contextual-relevance of infant obesity prevention programs for underserved populations, needs and preferences among community stakeholders and the focal population should be used as a roadmap for intervention development.


Subject(s)
Pediatric Obesity , Infant , Female , Pregnancy , Humans , Pediatric Obesity/prevention & control , Needs Assessment , Mothers , Poverty , Counseling
3.
Curr Opin Psychol ; 55: 101695, 2024 02.
Article in English | MEDLINE | ID: mdl-38128446

ABSTRACT

We theorize that expressing emotion often will enhance listening and responsiveness in communal relationships because the nature of cooperation called for in communal relationships often matches five functions that expressing emotion can serve. The same is less frequently true for other types of relationships.


Subject(s)
Emotions , Interpersonal Relations , Humans
4.
Nephrol Nurs J ; 50(4): 293-303, 2023.
Article in English | MEDLINE | ID: mdl-37695515

ABSTRACT

Studies suggest that the hemodialysis (HD) shift on which a patient dialyzes impacts sleep disorders, depressive symptoms, quality of life, and mortality. However, study findings have been limited and inconsistent. The purpose of this project was to determine the relationship between HD shifts (morning, afternoon, and evening), and depression and sleep quality disturbance. Forty-one participants were recruited to participate in this project. Participants on the third shift had the highest levels of depression (M = 11.67) and poorest sleep quality (M = 14.5), compared to those who dialyzed on the first shift, who had the best sleep quality scores (M = 8.31; p = 0.013) and depression scores (M = 4.25). A significant positive correlation was observed between depression and sleep quality scores (r = 0.64; p < 0.001). Results indicate the HD shift can play a significant role in sleep disturbances and depression in patients on HD. Further studies need to be conducted to validate these findings.


Subject(s)
Kidney Failure, Chronic , Sleep Wake Disorders , Humans , Renal Dialysis/adverse effects , Kidney Failure, Chronic/complications , Kidney Failure, Chronic/therapy , Depression/etiology , Quality of Life , Sleep Wake Disorders/etiology , Sleep
5.
Curr Opin Psychol ; 52: 101611, 2023 08.
Article in English | MEDLINE | ID: mdl-37354571

ABSTRACT

Two motivations linked to developing and maintaining communal relationships include caring for others' welfare and striving to be viewed as a desirable communal relationship partner. We review evidence suggesting that both motivations promote high-quality listening. Furthermore, high-quality listening signals listeners' prosocial motivation and enhances the perceived relational value of both listeners and speakers. Thus, high-quality listening likely functions as an effective strategy in the formation and maintenance of communal relationships. This review suggests several directions for future research, including longitudinal research on the role of listening in relationship development, accuracy and bias in detecting and interpreting listening, the influence of conversation topic on listening, the role of people's beliefs about the usefulness of listening, and individual and relationship factors that determine people's motivations for listening.


Subject(s)
Communication , Motivation , Humans
6.
Behav Brain Sci ; 46: e58, 2023 05 08.
Article in English | MEDLINE | ID: mdl-37154375

ABSTRACT

We agree with Grossmann that fear often builds cooperative relationships. Yet he neglects much extant literature. Prior researchers have discussed how fear (and other emotions) build cooperative relationships, have questioned whether fear per se evolved to serve this purpose, and have emphasized that human cooperation takes many forms. Grossmann's theory would benefit from a wider consideration of this work.


Subject(s)
Emotions , Fear , Male , Humans , Fear/psychology
7.
Perspect Psychol Sci ; 17(5): 1359-1380, 2022 09.
Article in English | MEDLINE | ID: mdl-35436157

ABSTRACT

Selfishness is central to many theories of human morality, yet its psychological nature remains largely overlooked. Psychologists often draw on classical conceptions of selfishness from evolutionary biology (i.e., selfish gene theory), economics (i.e., rational self-interest), and philosophy (i.e., psychological egoism), but such characterizations offer limited insight into the psychology of selfishness. To address this gap, we propose a novel framework in which selfishness is recast as a psychological construction. From this view, selfishness is perceived in ourselves and others when we detect a situation-specific desire to benefit the self that disregards others' desires and prevailing social expectations for the situation. We argue that detecting and deterring such psychological selfishness in both oneself and others is crucial in social life-facilitating the maintenance of social cohesion and close relationships. In addition, we show how using this psychological framework offers a richer understanding of the nature of human social behavior. Delineating a psychological construct of selfishness can promote coherence in interdisciplinary research on selfishness and provide insights for interventions to prevent or remediate the negative effects of selfishness.


Subject(s)
Biological Evolution , Social Behavior , Humans , Philosophy
8.
Conserv Biol ; 36(4): e13896, 2022 08.
Article in English | MEDLINE | ID: mdl-35146804

ABSTRACT

The International Union for Conservation of Nature's Red List of Threatened Species (IUCN Red List) is the world's most comprehensive information source on the global conservation status of species. Governmental agencies and conservation organizations increasingly rely on IUCN Red List assessments to develop conservation policies and priorities. Funding agencies use the assessments as evaluation criteria, and researchers use meta-analysis of red-list data to address fundamental and applied conservation science questions. However, the circa 143,000 IUCN assessments represent a fraction of the world's biodiversity and are biased in regional and organismal coverage. These biases may affect conservation priorities, funding, and uses of these data to understand global patterns. Isolated oceanic islands are characterized by high endemicity, but the unique biodiversity of many islands is experiencing high extinction rates. The archipelago of Hawaii has one of the highest levels of endemism of any floristic region; 90% of its 1367 native vascular plant taxa are classified as endemic. We used the IUCN's assessment of the complete single-island endemic (SIE) vascular plant flora of Kauai, Hawaii, to assess the proportion and drivers of decline of threatened plants in an oceanic island setting. We compared the IUCN assessments with federal, state, and other local assessments of Kauai species or taxa of conservation concern. Finally, we conducted a preliminary assessment for all 1044 native vascular plants of Hawaii based on IUCN criterion B by estimating area of occupancy, extent of occurrence, and number of locations to determine whether the pattern found for the SIE vascular flora of Kauai is comparable to the native vascular flora of the Hawaiian Islands. We compared our results with patterns observed for assessments of other floras. According to IUCN, 256 SIE vascular plant taxa are threatened with extinction and 5% are already extinct. This is the highest extinction risk reported for any flora to date. The preliminary assessment of the native vascular flora of Hawaii showed that 72% (753 taxa) is threatened. The flora of Hawaii may be one of the world's most threatened; thus, increased and novel conservation measures in the state and on other remote oceanic islands are urgently needed.


La Lista Roja de la Unión Internacional para la Conservación de la Naturaleza (Lista Roja UICN) es la fuente más completa a nivel mundial de información sobre el estado de conservación de las especies. Las agencias gubernamentales y las organizaciones para la conservación dependen cada vez más de las valoraciones en esta lista para desarrollar sus políticas y prioridades de conservación; también los organismos de financiamiento usan las valoraciones como criterios de evaluación y los investigadores aplican metaanálisis a los datos de la lista para abordar preguntas fundamentales y aplicadas a las ciencias de la conservación. Sin embargo, las casi 143,000 valoraciones de la UICN representan sólo una fracción de la biodiversidad mundial y están sesgadas en cuanto a la cobertura regional y de organismos. Estos sesgos pueden afectar a las prioridades de conservación, al financiamiento y al uso de estos datos para entender los patrones globales. Las islas oceánicas aisladas se caracterizan por un alto endemismo, aunque la biodiversidad única de muchas de estas islas está experimentando un índice elevado de extinciones. El archipiélago de Hawái tiene uno de los niveles más altos de endemismo de cualquier región florística con el 90% de los 1,367 taxones nativos de flora vascular clasificado como endémico. Usamos las valoraciones de la UICN para todas las plantas vasculares endémicas de una sola isla (ESI) en Kauai, Hawái, para evaluar la proporción y los impulsores de la declinación de plantas amenazadas en el entorno de una isla oceánica. Comparamos las valoraciones de la UICN con las federales, estatales y otras valoraciones locales de las especies o taxones de Kauai que son de importancia para la conservación. Por último, realizamos una valoración preliminar para las 1,044 especies de plantas vasculares nativas de Hawái con base en el criterio B de la UICN mediante la estimación del área de ocupación, la extensión de la ocurrencia y el número de localidades para determinar si el patrón hallado para la flora vascular ESI de Kauai es comparable con la flora vascular nativa de las islas hawaianas. Comparamos nuestros resultados con los patrones observados en las valoraciones de otras floras. De acuerdo con la UICN, el 95% de los taxones de plantas vasculares ESI de Kauai están amenazadas y el 5% ya se encuentra extinto. A la fecha, este es el riesgo de extinción más alto reportado para cualquier flora. La valoración preliminar de la flora vascular nativa de Hawái mostró que el 72% (753 taxones) se encuentra amenazado. La flora de Hawái puede ser una de las más amenazadas a nivel mundial; por lo tanto, se necesitan urgentemente medidas novedosas e incrementadas en el estado y en otras islas oceánicas remotas.


Subject(s)
Conservation of Natural Resources , Endangered Species , Animals , Biodiversity , Conservation of Natural Resources/methods , Extinction, Biological , Hawaii , Plants
9.
Health Psychol ; 40(11): 764-773, 2021 Nov.
Article in English | MEDLINE | ID: mdl-34914482

ABSTRACT

OBJECTIVE: Theories suggest that laughter decreases negative affect and enhances social bonds; however, no studies have examined the benefits of laughter on stress biomarkers in dyads. This study examined the hypotheses that individual and shared laughter would be associated with lower blood pressure reactivity and decreased self-reported and perceived partner distress for the target and spouse in a social support interaction. METHOD: One hundred seventy-three middle-aged and older adult couples from a larger study were video-recorded, and their blood pressure was monitored continuously in the laboratory during a resting baseline, during a social support interaction in which they discussed a target's fear related to aging, and while playing a game (used as a comparison). Both partners self-reported their own and perceived partner distress after the support interaction. Laughter behavior was coded using the Facial Action Coding System criteria. RESULTS: According to Actor Partner Interdependence Models, during the support interaction, the more the target laughed, the lower the spouse's systolic blood pressure was (partner effect). Also, greater laughter was associated with less self-reported and perceived partner distress for targets and spouses (actor effects). There were no other significant associations between individual laughter, shared laughter, systolic or diastolic blood pressure, and distress. Models controlled for gender, marital satisfaction, baseline blood pressure, and the target's baseline distress rating of their fear. CONCLUSIONS: In social support interactions, targets' laughter may have short-term blood pressure benefits for caregiving spouses and distress reducing benefits for both spouses. (PsycInfo Database Record (c) 2021 APA, all rights reserved).


Subject(s)
Laughter , Aged , Blood Pressure , Humans , Middle Aged , Social Support , Spouses
10.
Nat Commun ; 12(1): 5776, 2021 10 01.
Article in English | MEDLINE | ID: mdl-34599174

ABSTRACT

Judgments of whether an action is morally wrong depend on who is involved and the nature of their relationship. But how, when, and why social relationships shape moral judgments is not well understood. We provide evidence to address these questions, measuring cooperative expectations and moral wrongness judgments in the context of common social relationships such as romantic partners, housemates, and siblings. In a pre-registered study of 423 U.S. participants nationally representative for age, race, and gender, we show that people normatively expect different relationships to serve cooperative functions of care, hierarchy, reciprocity, and mating to varying degrees. In a second pre-registered study of 1,320 U.S. participants, these relationship-specific cooperative expectations (i.e., relational norms) enable highly precise out-of-sample predictions about the perceived moral wrongness of actions in the context of particular relationships. In this work, we show that this 'relational norms' model better predicts patterns of moral wrongness judgments across relationships than alternative models based on genetic relatedness, social closeness, or interdependence, demonstrating how the perceived morality of actions depends not only on the actions themselves, but also on the relational context in which those actions occur.


Subject(s)
Judgment/physiology , Humans , Interpersonal Relations , Morals , Social Perception
12.
Mindfulness (N Y) ; 11(4): 917-927, 2020 Apr.
Article in English | MEDLINE | ID: mdl-33343762

ABSTRACT

OBJECTIVE: We examined the feasibility and explored the physical, psychological, relational, and biological effects of Mindfulness-Based Stress Reduction (MBSR), an 8-week standardized mindfulness program, involving older married couples (60 years or older) with metabolic syndrome (one or both partners had metabolic syndrome). We also explored gender differences. METHODS: A pilot randomized controlled trial (RCT) compared MBSR to a Wait List Control (WLC) arm at baseline, post-intervention, and 3-month follow-up clinic visits. Twenty-two spouses (11 couples) self-reported stress, physical and mental functioning, mindfulness, and relationship satisfaction at each time point. Fasting glucose, cholesterol, triglycerides, blood pressure, weight, and waist circumference were measured. MBSR couples answered questions about partner influences on participation, adherence, and practice at the post-intervention visit. RESULTS: In terms of adherence to MBSR sessions, four of the six couples attended all 10 sessions; one couple attended 7; and one wife attended 6 and her husband attended 5 sessions. In terms of efficacy, there were no significant intervention effects; however, there were significant gender by intervention effects. Pre- to post-intervention, MBSR wives displayed greater increases in physical functioning (ß = 1.18, t(36) = 3.17, p = .003) and relationship satisfaction (ß = .72, t(36) = 2.81, p = .007) than WLC wives. Effects for husbands were not significant. Qualitatively, participants reported encouragement and increased relationship closeness. CONCLUSIONS: Engaging in MBSR as a couple to address symptoms of metabolic syndrome was well-received and feasible. Preliminary effects suggest more benefits for wives than husbands in terms of physical functioning and relational well-being.

13.
J Pastoral Care Counsel ; 74(4): 241-249, 2020 Dec.
Article in English | MEDLINE | ID: mdl-33228484

ABSTRACT

In Alberta, Canada, supervised pastoral education has grown and thrived for over 50 years. In 2008, due to healthcare funding changes, it was nearly eliminated. This article describes regenerative processes by which former structures have transformed into a new configuration for supervised pastoral education program delivery, including innovative roles for spiritual care preceptors and theological colleges. Out of the ashes, a phoenix is rising. It is called the Alberta Consortium for Supervised Pastoral Education program.


Subject(s)
Pastoral Care/economics , Pastoral Care/education , Pastoral Care/organization & administration , Program Development , Alberta , Humans
14.
Ann Epidemiol ; 52: 26-34, 2020 12.
Article in English | MEDLINE | ID: mdl-33010417

ABSTRACT

PURPOSE: The purpose of this study was to examine how combinations of adverse childhood events (ACEs) contribute to the risk of postpartum depression and the mediating role of prenatal social support. METHODS: The Adverse Childhood Experiences Scale Questionnaire and the Edinburgh Postnatal Depression Scale Questionnaire were used to measure the study's exposure and outcome. Among a cohort of 419 mothers enrolled in a home visiting (HV) program, latent class analyses were used to identify classes of ACEs exposure. General linear models assessed the risk of postpartum depression, and prenatal social support was examined as a mediator. RESULTS: Four distinct classes of ACE exposure were identified. On the Edinburgh Postnatal Depression scale, mothers who were classified in Classes 1-3 scored higher by 2.6-4.4 points compared with women in Class 0. ACE class was found to be indirectly associated with postpartum depression scores through prenatal social support. CONCLUSIONS: Identifying combinations of ACEs in an HV program has the potential to improve the characterization of ACEs among low-income perinatal women in the United States. Elucidating how these combinations contribute to the risk of postpartum depression has the potential to identify women at increased risk, which can help HV programs prioritize prevention efforts.


Subject(s)
Adult Survivors of Child Adverse Events/psychology , Adverse Childhood Experiences/psychology , Child Abuse/psychology , Depression, Postpartum/diagnosis , Social Support , Adult , Adult Survivors of Child Adverse Events/statistics & numerical data , Adverse Childhood Experiences/statistics & numerical data , Child , Child Abuse/statistics & numerical data , Cohort Studies , Depression, Postpartum/epidemiology , Depression, Postpartum/psychology , Female , Humans , Life Change Events , Postpartum Period , Prenatal Care , Psychiatric Status Rating Scales , Retrospective Studies , Socioeconomic Factors , Surveys and Questionnaires
15.
Behav Brain Sci ; 43: e66, 2020 04 30.
Article in English | MEDLINE | ID: mdl-32349849

ABSTRACT

Tomasello argues in the target article that a sense of moral obligation emerges from the creation of a collaborative "we" motivating us to fulfill our cooperative duties. We suggest that "we" takes many forms, entailing different obligations, depending on the type (and underlying functions) of the relationship(s) in question. We sketch a framework of such types, functions, and obligations to guide future research in our commentary.


Subject(s)
Morals , Psychology, Social , Moral Obligations , Problem Solving
16.
Clin Psychol Rev ; 76: 101826, 2020 03.
Article in English | MEDLINE | ID: mdl-32058881

ABSTRACT

Depression is characterized by increased levels of negative affect and decreased levels of positive affect. Prior research shows that individual differences in emotion regulation play an important role in understanding sustained negative affect within the disorder; yet, much less is known about the regulation of positive emotion in depression. The current paper utilizes emotion regulation models that synthesizes multiple emotion processes, including what people want to feel (emotion preferences) and the ways in which people typically respond to emotion (habitual use of emotion regulation strategies), to increase our understanding of positive emotion in depression. In doing so, we propose that depression is associated with relative reductions in the preference for positive emotion; these reductions may therefore increase the habitual use of emotion regulation strategies that serve to down-regulate positive emotion and decrease the use of strategies that serve to up-regulate positive emotion. Dysfunction in habitual emotion regulation strategy use may, in turn, contribute to the relatively low levels of positive emotion within the disorder. The paper also discusses important empirical gaps in the extant literature on emotion preferences and emotion regulation in depression and highlights novel treatment targets (e.g., emotion preferences) for interventions aimed at improving emotion dysfunction in depression.


Subject(s)
Depression/psychology , Emotional Regulation , Emotions , Humans
17.
Emotion ; 20(3): 329-342, 2020 Apr.
Article in English | MEDLINE | ID: mdl-31414835

ABSTRACT

When do people express their emotions to other people and when do they choose not to do so? Emotional experience-positive or negative-often leads people to reveal their feelings to others, especially to close relationship partners. Although emotional expression has been incorporated into recent dyadic models of emotion regulation, little research has examined the specific interpersonal processes responsible for facilitating or inhibiting emotional expression. This article reports results from a pair of methodologically distinct studies examining the impact of perceived partner responsiveness (PPR) on emotional expression. The results of Study 1, a 2-week daily diary study, demonstrated that within-person variations in the perceived responsiveness of a close partner were associated with corresponding day-to-day variations in emotional expression to that partner. In Study 2, in the context of a stressful situation, we manipulated perceptions of a romantic partner's responsiveness and then measured emotion expression toward that partner. Higher levels of experimentally induced PPR causally led to greater expression of anxiety. Together, these studies identify PPR as an important interpersonal mechanism underlying emotional expression. (PsycInfo Database Record (c) 2020 APA, all rights reserved).


Subject(s)
Expressed Emotion/physiology , Interpersonal Relations , Stress, Psychological/psychology , Adolescent , Adult , Female , Humans , Male , Young Adult
18.
Emotion ; 20(3): 353-367, 2020 Apr.
Article in English | MEDLINE | ID: mdl-31368745

ABSTRACT

Partners' negative emotions communicate social information necessary for individuals to respond appropriately to important relational events. Yet, there is inconsistent evidence regarding whether partners' emotional expression enhances accurate perceptions of partners' emotions. The current studies make methodological and theoretical extensions to the extant literature by directly assessing whether partners' emotional expression during relationship interactions predicts 2 types of accuracy relevant to the theorized interpersonal functions of negative emotions: tracking accuracy and directional bias. In Studies 1 and 2, both members of recruited couples reported on their own negative emotions, disclosure of emotions, and perceptions of their partners' negative emotions during relationship interactions at the end of each day for 21 days. In Study 3, couples engaged in an emotionally relevant discussion in the laboratory. Participants immediately reviewed their discussions and rated their own negative emotions and perceptions of their partners' negative emotions within each 30-s segment of the discussion. Independent coders rated the degree to which each person expressed their emotions during the discussion. In all three studies, partners' greater emotional expression predicted perceivers more accurately tracking partners' negative emotions (greater tracking accuracy). High levels of partners' emotional expression also predicted perceivers overestimating partners' negative emotions (greater directional bias). This expression-perception pattern should support the interpersonal function of negative emotions by orienting perceivers to important emotional events that would be costly to overlook. The results, considered in the context of prior research, highlight the importance of matching methodological approaches with the theoretical processes under investigation. (PsycInfo Database Record (c) 2020 APA, all rights reserved).


Subject(s)
Interpersonal Relations , Adult , Expressed Emotion , Female , Humans , Male , Perception , Young Adult
19.
J Biomech ; 98: 109462, 2020 01 02.
Article in English | MEDLINE | ID: mdl-31718820

ABSTRACT

Mechanical testing of soft tissues would ideally rely on using fresh specimens. In the event that fresh tissues are not readily available, alternative measures, such as storing fresh specimens at -80 °C, could be considered. Previous studies have shown that changes in the mechanical properties of the tissues due to freezing could be tissue-dependent. Prior to our study, however, such information was not available for the tricuspid valve leaflets. As such, for the first time, we examined whether fresh porcine specimens tested in a biaxial tensile machine would offer comparable results after being frozen at -80 °C. The stress-strain response of the tricuspid valve leaflets displayed no major deviation of the post-frozen leaflets as compared to fresh leaflets. We further compared the radial and circumferential strains as an indicator of deformation at similar stress states in fresh and thawed tissues, and we did not find any significant differences. Ice formation within the extra cellular matrix may modify the collagen fiber configuration, resulting in a slight change in the mechanical response. Nevertheless, our results indicated such a small deviation was negligible, thus enabling the possibility of using frozen porcine tricuspid valve specimens for future research.


Subject(s)
Cryopreservation , Freezing , Mechanical Phenomena , Tricuspid Valve , Animals , Biomechanical Phenomena , Stress, Mechanical , Swine , Time Factors
20.
Bioengineering (Basel) ; 6(3)2019 Aug 15.
Article in English | MEDLINE | ID: mdl-31443151

ABSTRACT

Since many soft tissues function in an isotonic in-vivo environment, it is expected that physiological osmolarity will be maintained when conducting experiments on these tissues ex-vivo. In this study, we aimed to examine how not adhering to such a practice may alter the mechanical response of the tricuspid valve (TV) anterior leaflet. Tissue specimens were immersed in deionized (DI) water prior to quantification of the stress-strain responses using an in-plane biaxial mechanical testing device. Following a two-hour immersion in DI water, the tissue thickness increased an average of 107.3% in the DI water group compared to only 6.8% in the control group, in which the tissue samples were submerged in an isotonic phosphate buffered saline solution for the same period of time. Tissue strains evaluated at 85 kPa revealed a significant reduction in the radial direction, from 34.8% to 20%, following immersion in DI water. However, no significant change was observed in the control group. Our study demonstrated the impact of a hypo-osmotic environment on the mechanical response of TV anterior leaflet. The imbalance in ions leads to water absorption in the valvular tissue that can alter its mechanical response. As such, in ex-vivo experiments for which the native mechanical response of the valves is important, using an isotonic buffer solution is essential.

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