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Methods Mol Biol ; 2480: 17-48, 2022.
Article in English | MEDLINE | ID: mdl-35616855

ABSTRACT

Nicotiana tabacum (the tobacco plant ) has numerous advantages for molecular farming, including rapid growth, large biomass and the possibility of both cross- and self-fertilization. In addition, genetic transformation and tissue culture protocols for regeneration of transgenic plants are well-established. Here, we describe the production of transgenic tobacco using Agrobacterium tumefaciens and the analysis of recombinant proteins, either in crude plant extracts or after purification, by enzyme-linked immunosorbent assays, sodium dodecyl sulfate polyacrylamide gel electrophoresis with western blotting and surface plasmon resonance.


Subject(s)
Agrobacterium tumefaciens , Nicotiana , Agrobacterium tumefaciens/genetics , Agrobacterium tumefaciens/metabolism , Blotting, Western , Plants, Genetically Modified , Recombinant Proteins/metabolism , Nicotiana/metabolism
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