The measurement of charge for induction-based fluidic MALDI dispense event and nanoliter volume verification in real time.

This study preliminarily investigates whether nanoliter volumes of concentrated polar liquids and organic monomers launched to targets using induction based fluidics (IBF) can be verified through the real time charge measurements. We show that using a nanoliter IBF dispensing device and nanocoulomb meter, charge measurements made on nanoliter drops in real time are correlated with surface area following Gauss's Law. We infer the "induction only" formation of the double layer showing the ability to determine nanoliter volumes, nearly instantaneously, in real time. We discuss the implications that these observations may have for on improving/monitoring MALDI quantitation and its quality control.

Dacryocystitis presenting as post-septal cellulitis: a case report.

Dacryocystitis is relatively common, the majority of patients present with pre-septal cellulitis and not an orbital abscess due to anatomical barriers. The authors report a case of dacryocystitis presenting as post-septal cellulitis in a postmenopausal lady with an underlying malignancy. Following antibiotic therapy and elective dacryocystorhinostomy the patient is still under follow-up, and has no further recurrence of symptoms. Orbital abscess in postmenopausal women presenting with dacryocystitis should be considered, as prompt recognition and early surgical intervention is required to prevent visual loss.

Evaluation of a point-of-care blood analyzer and determination of reference ranges for blood parameters in rockfish.

OBJECTIVE: To compare values of blood parameters in rockfish obtained by use of a point-of-care portable blood analyzer with values determined by a veterinary diagnostic laboratory, calculate reference ranges for various blood parameters in black rockfish, and compare values of blood parameters in clinically normal fish with those of fish with clinical abnormalities. DESIGN: Prospective study. ANIMALS: 41 captive adult black rockfish (Sebastes melanops) and 4 captive adult blue rockfish (Sebastes mystinus). PROCEDURE: Rockfish were anesthetized with tricaine methanesulfonate for collection of blood samples. Heparinized blood samples were immediately analyzed with a point-of-care analyzer. Blood sodium, potassium, chloride, urea nitrogen, and glucose concentrations; Hct; pH; partial pressure of carbon dioxide; total carbon dioxide concentration; bicarbonate concentration; base excess; and hemoglobin concentration were determined. A microhematocrit technique was used to determine PCV, and a refractometer was used to estimate total plasma protein concentration. Paired heparinized blood samples were transported to a veterinary diagnostic laboratory for analyses. RESULTS: Data obtained with the point-of-care analyzer were reproducible; however, values for most blood parameters were significantly different from those obtained by the veterinary diagnostic laboratory. Fish with poor body condition had several blood parameter values that were lower than corresponding values in clinically normal fish. CONCLUSIONS AND CLINICAL RELEVANCE: Point-of-care blood analyses may prove useful in rockfish. Point-of-care data for a large number of clinically normal fish must be obtained for reference ranges to be calculated, and further assessments of clinically abnormal fish are necessary to determine the relevance of the data.

Research as a catalyst for change: the transition from student to Registered Nurse.

This paper outlines the qualitative findings of a study exploring the transition from student to Registered Nurse and the catalytic effect of the way in which newly registered nurses are prepared for this role change. The study explored the experiences of one cohort of students undertaking the pre-registration Advanced Diploma in Nursing Studies at the University of Sheffield. Data were collected pre-qualification and post-qualification in order to compare the expectations and reality of the transition period. Two key aspects emerged, one relating to the experience of students undertaking the final module of the course and the other to preceptorship once qualified. These two areas of practice have been the focus of development following dissemination of the research findings in the local NHS Hospitals. The study suggests that the transition period remains very stressful for some nurses, because of both pre-registration educational issues and the level of support received once qualified. Careful planning of the student experience in the final year and addressing inconsistencies within the preceptorship programmes of newly qualified nurses are needed. The study is stimulating change in the local NHS hospitals, with the aim of enhancing the overall experience of student nurses and newly qualified staff alike.