ABSTRACT
BACKGROUND: Meligethes are pollen-beetles associated with flowers of Rosaceae as larvae. This genus currently consists of 63 known species in two subgenera, Meligethes and Odonthogethes, predominantly occurring in the eastern Palaearctic. We analyzed 74 morphological and ecological characters (169 states) of all species, as well as of 11 outgroup species from 7 Meligethinae genera (including Brassicogethes), to investigate their phylogeny. We also conducted a parallel molecular analysis on 9 Meligethes, 9 Odonthogethes, 3 Brassicogethes and 2 Meligethinus species based on DNA sequence data from mitochondrial (COI, 16S) and nuclear (CAD) genes. RESULTS: Morphological phylogenetic reconstructions supported the monophyly of the whole genus and clades corresponding to purported subgenera Meligethes s.str. and Odonthogethes. Main species-groups were mostly confirmed, however some unresolved polytomies remained. Molecular data placed members of Brassicogethes (including 42 mostly W Palearctic species associated with Brassicaceae) as sister to Odonthogethes, with this clade being sister to Meligethes s.str. This phylogenetic scenario suggests that monophyletic Meligethes s.str., Odonthogethes and Brassicogethes should be regarded alternatively as three subgenera of a monophyletic Meligethes, or three genera in a monophyletic genus-complex, with mutually monophyletic Brassicogethes and Odonthogethes. Molecular analyses estimated the origin of this lineage at ca. 14-15 Mya from a common stem including Meligethinus. CONCLUSIONS: We hypothesize that the ancestor of Meligethes specialized on Rosaceae in the Middle Miocene (likely in Langhian Age) and subsequently radiated during Late Miocene and Plio-Pleistocene maintaining a trophic niche on this plant family. This radiation was primarily due to geographic isolation in E Asiatic mountain systems. Combined evidence from morphology, ancestral state parsimony reconstruction of host-plant associations and molecular evidence suggested that Rosoideae (Rosa spp.) represented the ancestral hosts of Meligethes s.str., followed by an independent shift of ancestral Odonthogethes (ca. 9-15 Mya) on Rubus (Rosoideae) and members of Rosaceae Spiraeoideae. Other ancestral Odonthogethes probably shifted again on the unrelated plant family Brassicaceae (maybe 8-14 Mya in S China), allowing a rapid westward radiation of the Brassicogethes clade.
ABSTRACT
The genus Meligethinus Grouvelle, 1906 represents a small group of pollen beetles, including some twenty species distributed from southern Palaearctic areas to northern Oriental and Afrotropical regions. All constituent species appear to be strictly associated as larvae and adults to male inflorescences of palms (Monocots: Arecaceae). Two new species of this genus (Meligethinus mondlanei sp. nov. and M. hamerlae sp. nov.) were recently discovered in southern Mozambique, and are described herein. Both are associated as larvae with male inflorescences of the widespread eastern African palm Phoenix reclinata Jacq. The two new species are compared with related taxa from central Africa. Additionally, the presence of additional species of the same genus in southern Mozambique is reported, and the local associations of several Meligethinus species are discussed.
Subject(s)
Arecaceae , Coleoptera , Animals , Larva , Male , Mozambique , PollenABSTRACT
Five new species of the genus Lamiogethes Audisio Cline are described from western, central and southern China: L. falcatus sp. n. (Sichuan), L. hastipenis sp. n. (Hubei), L. sagittalis sp. n. (Shaanxi), L. unditibiis sp. n. (Chongqing), and L. limaelytralis sp. n. (Sichuan). The first four new species appear to be morphologically related to Lamiogethes ancestor (Kirejtshuk, 1980) (comb. n.) from Central China, and allied taxa. Lamiogethes limaelytralis sp. n. appears to be more isolated, probably with relationships to the recently described L. forcipenis Liu, Huang, Cline Audisio, 2017 and L. convexistrigosus Liu, Huang, Cline Audisio, 2017 from Sichuan. All new species differ from other closely related taxa by details of the body shape and color, as well as the shape of the male and/or female genitalia.
Subject(s)
Coleoptera , Animal Distribution , Animals , China , Color , Female , MaleABSTRACT
Meligethes (Odonthogethes) trapezithorax sp. n., M. (O.) tricuspidatus sp. n., and M. (O.) luteomaculatus sp. n. are described from Hubei Province, China, based on a series of recently collected specimens. The new species appear morphologically closely related to M. (O.) brassicogethoides Audisio, Sabatelli Jelínek, 2015 from Yunnan [M. (O.) trapezithorax sp. n.], M. (O.) occultus Audisio, Sabatelli Jelínek, 2015 from Yunnan [M. (O.) tricuspidatus sp. n.], as well as M. (O.) pallidoelytrorum Chen Kirejtshuk, 2013 from Sichuan and M. (O.) simulator Audisio, Sabatelli Jelínek, 2015 from Shaanxi and Gansu [M. (O.) luteomaculatus sp. n.]. The new species clearly differ from their closely related taxa by details of body shape and color, and the distinct shape of the male and/or female genitalia. The previously unknown male of the rare and localized M. (M.) elytralis Audisio, Sabatelli Jelínek, 2015 from Sichuan is described for the first time, and new distributional data of other Chinese species of the genus are presented, including M. (M.) semenovi Kirejtshuk, 1979 from Hubei, M. (M.) macrofemoratus Liu, Yang, Huang, Jelínek Audisio, 2016 from Hubei, M. (M.) auropilosus Liu,Yang, Huang, Jelínek Audisio, 2016 from Hubei, M. (O.) flavicollis Reitter, 1873 from Chongqing, M. (O.) wagneri Rebmann, 1956 from Shaanxi, M. (O.) pectoralis Rebmann, 1956 from Guizhou, and M. (O.) pseudopectoralis Audisio, Sabatelli Jelínek, 2015 from Sichuan. Meligethes (M.) cinereoargenteus is moved from the Meligethes nepalensis species group to the Meligethes auripilis species group. Additional data on geographic distribution and larval ecology of other species in the genus are also reported. An updated species checklist of the genus Meligethes is presented.
