Time course of neocortical graft innervation by AChE-positive fibers.

Acetylcholinesterase (AChE)-containing axons are the only extrinsic fibers projecting to the adult cortex that readily innervate embryonic cortical grafts up to normal densities without prior manipulation of the host brain. In the present paper we compare the time course of AChE-positive fiber innervation in the normal mouse cortex with that seen in neocortical grafts by using AChE histochemistry as a marker for presumed cholinergic fibers. Donor tissue was taken at two different stages of gestation; before (embryonic days 12-14, or E12-14) and after (E17-19) the cortical plate is formed. Three features are analyzed: 1) the distribution and density of AChE-containing fibers, 2) the presence of AChE-positive cells, and 3) the distribution of butyrylcholinesterase (BuChE)-positive elements. The modification of Koelle's method used for AChE localization showed AChE-positive fibers in developing parietal neocortex as early as E18-19. The distribution of AChE-labeled fibers in the normal cortex achieves the mature pattern by the end of the third postnatal week. The rate of innervation of transplants takes longer and depends on the age of the donor tissue. Tissue from both donor ages first showed AChE-positive fibers crossing the host-transplant interface by 7 days postsurgery. E17-19 tissue approaches the density of AChE-positive fibers in the normal adult cortex by 15 weeks after grafting, whereas the E12-14 donor tissue does not approach normal innervation densities until after 20 weeks. While the degree of innervation in the E12-14 donor tissue never equalled the surrounding adult cortex within our range of survival times, a few of the E17-19 transplants did develop densities equal to that of the host cortex. AChE-positive cells are first detectable in the normal parietal cortex on the day of birth, peak by the end of the first postnatal week, and then decline in number to the low levels of the mature cortex after the second postnatal week. Grafted cells in E12-14 tissue stain lightly for AChE by 7 days postsurgery, achieve maximal densities by 3 weeks, and become markedly reduced in number and density by 10 weeks. Cells in E17-19 tissue are lightly reactive by 7 days postsurgery, reach maximal numbers by 2 weeks postsurgery, and become similar in number and density to those seen in the mature cortex after 4 weeks. The appearance of BuChE-reactive blood vessels, neurons, and glia in both normal development and in the transplants is described and discussed.(ABSTRACT TRUNCATED AT 400 WORDS)

Somatosensory projections to the superior colliculus of the anaesthetized cat.

1. Experiments in anaesthetized cats have shown that the superior colliculus receives deep afferent input from the forelimb and hindlimb, but not from the large superficial neck muscles. 2. Neuronal activity in the superior colliculus is readily elicited by electrical stimulation of C2 and C3 cutaneous nerves. A significant proportion of neurones so activated have multiple receptive fields and some with no identifiable receptive fields in regions innervated by C2 and C3 nerves have receptive fields elsewhere on the body surface. Many collicular neurones activated by C2 and C3 stimulation had no identifiable receptive fields. 3. Natural stimuli to the limbs, hitherto believed to activate only cutaneous receptors, are sufficient to activate deep receptors which contribute to the neuronal responses in the superior colliculus elicited by the natural stimulus. These same natural stimuli set up transmitted vibration adequate to excite receptors some distance from the applied stimulus. 4. No evidence was found for a rigorous somatotopy in the superior colliculus. The great majority of neurones received trigeminal input which is widely distributed throughout the superior colliculus. 5. Tactile stimuli to the face are most effective in eliciting unit activity in the superior colliculus and many neurones activated by these stimuli were shown to be tectospinal neurones. In particular, the specialized receptors of the face, including the glabrous skin of the snout (the planum nasale) and the vibrissae, are major sources of input to collicular neurones including tectospinal neurones. 6. It is suggested that a major role of the superior colliculus is in the organization of head movements associated with the use of the specialized receptor organs of the face in exploratory behaviours. The superior colliculus may also be involved in the organization of aversion movements of the head.