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4.
Exp Gerontol ; 156: 111607, 2021 12.
Article in English | MEDLINE | ID: mdl-34715304

ABSTRACT

The effects of aging on ROS production and DNA damage were assessed in hematopoietic stem cells (HSCs) from apolipoprotein E-deficient (ApoE-/-) mice (2-, 12- and 24-month-old), a traditional experimental model of atherogenic dyslipidemia. HSCs from aged ApoE-/- mice were associated with increased ROS levels, leading to loss quiescence, DNA damage, apoptosis and telomere shortening. The concurrence of lack of ApoE and aging result in exhaustion and senescence of HSCs accompanied by increased oxidative stress and inflammation. Therefore, our data open avenues to a better understanding of age-related changes and genetic factors, which may synergistically compromise the efficacy of aged HSC recovery and/or transplantation.


Subject(s)
Hematopoietic Stem Cells , Oxidative Stress , Aging , Animals , Apolipoproteins E/genetics , Cellular Senescence , DNA Damage , Mice , Mice, Inbred C57BL , Reactive Oxygen Species
8.
Food Chem ; 345: 128778, 2021 May 30.
Article in English | MEDLINE | ID: mdl-33310250

ABSTRACT

The innovative combination of ultrasound (Us) with a thermal exchanger to produce high quality extra virgin olive oil (EVOO) was studied using Nuclear Magnetic Resonance (NMR) spectroscopy and multivariate analysis (MVA). Major and minor metabolomic components of Apulian Coratina EVOO obtained using the two methods were compared. Early and late olive ripening stages were also considered. An increased amount of polyphenols was found for EVOOs obtained using the Us with respect to the conventional method for both early and late ripening stages (900.8 ± 10.3 and 571.9 ± 9.9 mg/kg versus 645.1 ± 9.3 and 440.8 ± 10.4 mg/kg). NMR spectroscopy showed a significant increase (P < 0.05) in polyunsaturated fatty acids (PUFA) as well as in the tyrosol and hydroxytyrosol derivatives, such as oleocanthal, oleacein, and elenolic acid, for both ripening stages. In conclusion, NMR spectroscopy provides information about the metabolomic components of EVOOs to producers, while the Us process increases the levels of healthy bioactive components.


Subject(s)
Food Industry , Magnetic Resonance Spectroscopy , Metabolomics , Olive Oil/metabolism , Temperature , Ultrasonic Waves , Multivariate Analysis
10.
Environ Pollut ; 254(Pt A): 112977, 2019 Nov.
Article in English | MEDLINE | ID: mdl-31377326

ABSTRACT

Glyphosate, as a broad-spectrum herbicide, is frequently detected in water and several studies have investigated its effects on several freshwater aquatic organisms. Yet, only few investigations have been performed on marine macroalgae. Here, we studied both the metabolomics responses and the effect on primary production in the endemic brown algae Fucus virsoides exposed to different concentration (0, 0.5, 1.5 and 2.5 mg L-1) of a commercial glyphosate-based herbicide, namely Roundup®. Our results show that Roundup® significantly reduced quantum yield of photosynthesis (Fv/Fm) and caused alteration in the metabolomic profiles of exposed thalli compared to controls. Together with the decrease in the aromatic amino acids (phenylalanine and tyrosine), an increase in shikimate content was detected. The branched-amino acids differently varied according to levels of herbicide exposure, as well as observed for the content of choline, formate, glucose, malonate and fumarate. Our results suggest that marine primary producers could be largely affected by the agricultural land use, this asking for further studies addressing the ecosystem-level effects of glyphosate-based herbicides in coastal waters.


Subject(s)
Fucus/metabolism , Glycine/analogs & derivatives , Herbicides/toxicity , Water Pollutants, Chemical/toxicity , Agriculture , Animals , Ecosystem , Fresh Water/chemistry , Glycine/toxicity , Metabolomics , Phenylalanine/metabolism , Photosynthesis/drug effects , Shikimic Acid/metabolism , Tyrosine/metabolism , Glyphosate
11.
Minerva Ginecol ; 66(4): 383-9, 2014 Aug.
Article in English | MEDLINE | ID: mdl-25020057

ABSTRACT

Today, 88% of pregnancies has a physiological course during which just basic care, while in 12% of cases there is a high-risk pregnancy that requires additional assistance and specific. The approach that should be used is that of supervision in all pregnant women considering their potential to have a normal pregnancy until there is no clear evidence to the contrary. Pregnancy is considered at risk if there are medical conditions that may affect maternal or fetal health or life of the mother, fetus or both. Among the risk factors for pregnancy there is first the woman's age, in fact the increase in high-risk pregnancies in the last 20 years is attributable to the increase in the average age of women who face pregnancy. In addition, the diet is very important during pregnancy and diabetes or autoimmune diseases often lead to the failure of a pregnancy. Risk factors for pregnancy, also, are the complications that occur during its course as hypertension during pregnancy, and infectious diseases. Fears and anxieties typical of a high-risk pregnancy prevent the couple to live happily in the months of gestation. Effective communication, control and early detection are important tools that doctors must be able to ensure that women in order to plan the best treatment strategies and to minimize the risks of maternal and / or fetal.


Subject(s)
Pregnancy Complications/therapy , Pregnancy, High-Risk , Prenatal Care/methods , Communication , Diet , Female , Humans , Pregnancy , Pregnancy Complications/epidemiology , Risk Factors
12.
Minerva Ginecol ; 66(3): 299-301, 2014 Jun.
Article in English | MEDLINE | ID: mdl-24971785

ABSTRACT

AIM: Polycystic ovary syndrome (PCOS) is one of the most common causes of ovulatory infertility. It is an endocrine disorders characterized by a high level of male hormones (androgens) and frequent anovulatory cycles associated with multiple ovarian microcysts. The aim of this paper was to evaluate effects of a Clomiphene citrate alone versus a combined treatment (Metformin and Clomiphene citrate). METHODS: A total of 60 women with PCOS and infertility were evaluated. Inclusions criteria were: age 26-34 years, nulliparity, above 3 years of sterility, multiple ovarian microcysts, BMI>27.5, oligomenorrhea/amenorrhea, hyperandrogenism and normal male fertility. Four patients were excluded (renal damage 2, tubal occlusion 1 and Pelvic Inflammatory Disease 1). The remaining 56 were divided into 2 groups: group A were inducted with Clomiphene Citrate alone, while group B were inducted with Clomiphene citrate and Metformin. RESULTS: In group A we obtained ovulation in 20 women (71.4%), 8 pregnancies (28.5%) and one (3.5%) spontaneous abortion. In group B we obtained ovulation in 24 women (85.7%), 15 pregnancies (53.5%) and no spontaneous abortions. CONCLUSION: Combined treatment was found to be more effective (53.5) in improving pregnancy rate compared to monotherapy (28.5%).


Subject(s)
Clomiphene/administration & dosage , Infertility, Female/drug therapy , Metformin/administration & dosage , Ovulation Induction/methods , Adult , Clomiphene/therapeutic use , Drug Therapy, Combination , Female , Fertility Agents, Female/administration & dosage , Fertility Agents, Female/therapeutic use , Humans , Hypoglycemic Agents/administration & dosage , Hypoglycemic Agents/therapeutic use , Infertility, Female/etiology , Metformin/therapeutic use , Polycystic Ovary Syndrome/complications , Pregnancy , Pregnancy Rate
13.
Minerva Ginecol ; 66(3): 303-7, 2014 Jun.
Article in English | MEDLINE | ID: mdl-24971786

ABSTRACT

AIM: Unexplained infertility affects 30% of infertile couples. Management depends on duration of infertility and age of female partner. Ovulation induction, together with intrauterine insemination, is commonly offered to couples with infertility of unknown origin. Intrauterine insemination gained its popularity because it is simple, non-invasive and cost-effective technique. The association with pharmacological stimulation is suitable to induce follicular maturation. The most used drugs to induce ovulation are clomiphene citrate and menopausal or recombinant gonodotropins. The aim of this paper was to evaluate the success rate after homologous intrauterine insemination (IUI) combined with menopausal gonadotropins stimulation. METHODS: A total of 90 couples were evaluated. Twelve couples (13.3%) were excluded from the treatment (6 vaginal infections, 4 tubal occlusions and 2 male infertility). Informed consent was applied for every couple. The remaining 78 couples were divided in two groups: group A (39 couples) were inducted with menopausal gonadotropins, while group B (39 couples) underwent placebo (multivitamin). RESULTS: In group A (gonadotropins) 25 (64.1%) pregnancies and 2 (5.1%) abortion were registered, while in group B (placebo) there were 7 (17.9%). pregnancies and 1 (2.5%) abortion. CONCLUSION: Ovulation inductions with menopausal gonadotropins, together with intrauterine insemination, improves fecundity in patients with infertility of unknown origin without recur to more invasive techniques.


Subject(s)
Fertility Agents, Female/therapeutic use , Insemination, Artificial/methods , Menotropins/therapeutic use , Ovulation Induction/methods , Adult , Female , Humans , Infertility/therapy , Male , Middle Aged , Pregnancy , Pregnancy Rate , Treatment Outcome
14.
Minerva Ginecol ; 66(3): 309-12, 2014 Jun.
Article in English | MEDLINE | ID: mdl-24971787

ABSTRACT

AIM: Among couples unable to conceive without any identifiable cause, 30% are defined as having unexplained infertility. Management depends on duration of infertility and age of female partner. In order to increase pregnancy rate, couples with infertility of unknown origin can undergo ovulation induction and intrauterine insemination. These techniques are able to increase pregnancy rate in case of unexplained infertility. Clomiphene citrate and menopausal or recombinant gonodotropins are the most used drugs to induce ovulation. Aim of the present study was to evaluate the success rate after homologous intrauterine insemination (IUI) combined with clomiphene citrate (CC) stimulation. METHODS: A total of 77 couples were evaluated. Nine couples (11.6%) were excluded from the treatment (vaginal infections 4, tubal occlusions 3 and male sterility 2). Informed consent was applied for every couple. The remaining 68 couples were divided in two groups: group A (34 couples) were inducted with CC, while group B (34 couples) underwent placebo (multivitamin). RESULTS: In group A (CC) 15 (44.1%) pregnancies and 3 (8.8%) abortion were registered, while in group B (placebo) there were 4 (11.7%) pregnancies and 1 (2.9%) abortion. CONCLUSION: Ovulation inductions with CC, together with intrauterine insemination, improves fecundity in patients with infertility of unknown origin with no need to recur to more invasive techniques.


Subject(s)
Clomiphene/therapeutic use , Fertility Agents, Female/therapeutic use , Insemination, Artificial/methods , Ovulation Induction/methods , Adult , Female , Humans , Infertility/etiology , Infertility/therapy , Male , Middle Aged , Pregnancy , Pregnancy Rate , Treatment Outcome
15.
G Chir ; 34(11-12): 323-5, 2013.
Article in English | MEDLINE | ID: mdl-24342161

ABSTRACT

Paratubal cysts represent approximately 10% of all adnexal masses. In most cases they are very small, but very few cases are reported in the literature where they exceed 15 cm of diameter. Furthermore, giant paratubal cysts complicated by bilateral hydronephrosis are unique. The Authors describe a case of a huge paratubal cyst (30 cm in diameter), in a 14 year old obese girl, treated by complete laparoscopic enucleation.


Subject(s)
Hydronephrosis/surgery , Laparoscopy , Parovarian Cyst/surgery , Adolescent , Female , Humans , Hydronephrosis/etiology , Parovarian Cyst/complications , Parovarian Cyst/pathology
16.
Radiol Med ; 118(5): 837-50, 2013 Aug.
Article in English | MEDLINE | ID: mdl-23090252

ABSTRACT

Kidney transplantation is currently the treatment of choice in most patients with end-stage chronic renal failure owing to the excellent results in terms of both graft and patient survival. However, surgical complications are still very frequent. Although urological (stricture, urinary fistulas, vesico-ureteral reflux) and lymphatic complications (lymphocoele) have a high incidence, they only rarely lead to graft loss. By contrast, vascular complications (stenosis, arterial and venous thrombosis, arterio-venous fistulas, pseudoaneurysms) are relatively rare, but potentially serious and may affect graft survival. Finally, medical complications such as acute tubular necrosis (ATN), rejection and de novo neoplasms may also arise in kidney transplantation. The purpose of this pictorial review is to illustrate the increasingly significant contribution of magnetic resonance angiography (MRA) in the management of complications of kidney transplantation, and emphasise how this method should now be considered a mandatory step in the diagnostic workup of selected cases. Moreover, the application and role in this setting of new magnetic resonance imaging (MRI) techniques, such as diffusion-weighted and blood oxygen level-dependent (BOLD) MRI, are also discussed.


Subject(s)
Kidney Failure, Chronic/surgery , Kidney Transplantation , Magnetic Resonance Angiography/methods , Postoperative Complications/diagnosis , Contrast Media , Humans
17.
J Neuroradiol ; 39(1): 44-50, 2012 Mar.
Article in French | MEDLINE | ID: mdl-21821290

ABSTRACT

Discography test associated with the scanner (discoscanner) is an exam that has been a renewed interest in recent few years. Thanks to the emergence of new interventions such as disc prosthesis, the procedures require confirmation of the disc level to deal with and the origin of discogenic symptoms. The aim of this paper is to describe the techniques, challenges and tips as well as the interpretation of functional and morphological examination.


Subject(s)
Intervertebral Disc/diagnostic imaging , Low Back Pain/diagnostic imaging , Lumbar Vertebrae/diagnostic imaging , Spinal Diseases/diagnostic imaging , Tomography, X-Ray Computed , Contrast Media , Humans , Iopamidol/analogs & derivatives , Magnetic Resonance Imaging , Pain Measurement
18.
J Neuroradiol ; 38(3): 178-82, 2011 Jul.
Article in French | MEDLINE | ID: mdl-21496925

ABSTRACT

Transfacet screws may be useful for stabilizing segments reconstructed with bone graft or cages, the role of supplementary posterior fixation, particularly minimally invasive techniques such as transfacetar percutaneous screws is relevant. To benefit from a mechanical fixation after anterior arthrodesis without the inconveniences of the open classical posterior surgical intervention, we have developed a new procedure performed under local anesthesia and CT guidance and based on the intra-articular application of screws. This study was designed to demonstrate the feasibility of using a CT-scan to perform posterior arthrodesis of the spine under local anesthesia.


Subject(s)
Arthrodesis/methods , Bone Screws , Intervertebral Disc Degeneration/surgery , Radiography, Interventional , Tomography, X-Ray Computed , Adult , Aged , Anesthesia, Local , Feasibility Studies , Female , Humans , Intervertebral Disc Degeneration/diagnostic imaging , Lumbar Vertebrae , Male , Middle Aged , Treatment Outcome
19.
J Radiol ; 91(11 Pt 1): 1103-11, 2010 Nov.
Article in French | MEDLINE | ID: mdl-21178873

ABSTRACT

Splenic artery aneurysms are now diagnosed more frequently thanks to the increase and improvement in different imaging techniques. In case of rupture they are potentially life threatening and thus in certain cases may require appropriate preventive treatment. This treatment should be offered to patients with suspected pseudoaneurysms, with an aneurysm larger than 20mm in diameter, or which is progressing. The development of interventional endovascular radiology has provided new therapeutic options for the management of aneurysms, by excluding the sac from the arterial circulation with coil embolisation or with a covered stent. The success rate of these treatments is between 75 and 100% with significantly less morbidity and mortality than with surgical techniques.


Subject(s)
Aneurysm/diagnosis , Aneurysm/therapy , Angiography , Embolization, Therapeutic/methods , Splenic Artery , Tomography, X-Ray Computed , Ultrasonography , Embolization, Therapeutic/adverse effects , Humans , Treatment Outcome
20.
Int Angiol ; 29(4): 355-61, 2010 Aug.
Article in English | MEDLINE | ID: mdl-20671654

ABSTRACT

AIM: We evaluated the changes of lipidic and coagulative pattern during menopause and the influence of hormone replacement therapy (HRT) on these parameters. METHODS: We considered 158 patients divided into 2 groups: Group I consisted of 127 women in physiological/surgical menopause and Group II of 31 women with childbearing potential. Subsequently, we considered a group III formed of 34 patients from menopausal women (group I) who underwent three months of HRT. We evaluated total-cholesterol (TC), HDL-cholesterol (HDL-C), LDL-cholesterol (LDL-C), triglycerides (TG), lipoprotein (a) (Lpa), fibrinogen, antithrombin III (ATIII), factor VII (FVII) and tissue factor pathway inhibitor (TFPI). RESULTS: We found a worse lipid profile in the post-menopausal group compared to controls (TC 243.8+/-29.7 vs 217.9+/-32.7 mg%, P=0.002; TG 121.5+/-68.4 vs 88.6+/-53.0 mg%, P=0.039; LDL-C 163.0+/-27.9 vs 136.2+/-29.6 mg%, P=0.004; HDL-C 60.9+/-14.9 vs 64.1+/-14.6 mg%, P=ns). With regard to the coagulative pattern, fibrinogen was significantly higher in the post-menopausal group (fibrinogen: 273.3+/-67.4 vs 243.8+/-39.5 mg%, P=0.013; ATIII 112.2+/-11.7 vs 117.5+/-12.7% %, P=0.059; FVII 121.6+/-11.3 vs 117.6+/-10.8 mg%, P=ns; TFPI activity 2.5+/-2.3 vs 2.1+/-1.1 U/mL, P=ns; TFPI antigen 120+/-38 vs 127+/-39 U/mL, P=ns). Comparing the same parameters, before and after three months of HRT, in patients of Group III we observed a significant improvement of TC and TG levels (TC from 232.3+/-42.7 to 215.2+/-37.6 mg%, P=0.0001; TG from 103.7+/-56.8 to 95.0+/-44.3 mg%, P=0.059; HDL-C from 62.3+/-12.9 to 63.6+/-12.6 mg%, P=ns; LDL-C from 149.3+/-38.7 to 132.6+/-34.5 mg%, P=0.0001). The following changes were observed with regard to coagulative parameters: fibrinogen from 270.9+/-69.4 to 253.2+/-56.2 mg%, P=0.07; ATIII from 113.5+/-11.4 to 110.8+/-13.2 mg%, P=0.198; FVII from 108.6+/-18.0 to 104.4+/-17.5 mg%, 0.014. TFPI activity from 2.6+/-2.3 to 2.3+/-1.4 U/ml, P=ns; TFPI antigen from 68+/-13 to 87+/-22 U/mL, P=0.001. CONCLUSION: Our data confirm the presence of an alteration in lipidic and coagulative pattern in post menopausal women and positive changes after HRT.


Subject(s)
Blood Coagulation/drug effects , Hormone Replacement Therapy , Lipids/blood , Adult , Aged , Antithrombin III/metabolism , Biomarkers/blood , Cholesterol/blood , Factor VIII/metabolism , Female , Fibrinogen/metabolism , Humans , Italy , Lipoprotein(a)/blood , Lipoproteins/blood , Middle Aged , Postmenopause , Time Factors , Treatment Outcome , Triglycerides/blood
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