ABSTRACT
BACKGROUND: Multiresistant bacterial strains tend to develop, especially enterobacteriacae, in intraabdominal infections. The aim of this study was to characterize the evolution of the bacterial biota in complicated appendicitis in children over the past 20 years and their acquired resistance rates to antibiotics. MATERIALS AND METHODS: All pediatric patients admitted in the emergency unit for complicated appendicitis were retrospectively reviewed during 3 periods: 1989 to 1991, 1999 to 2000, and 2009 to 2010. Results of peritoneal swabs were analyzed regarding bacterial species and resistance to antibiotics. Statistical significance was set at P < .05. RESULTS: Thirty-four, 48, and 85 patients from the 3 periods, respectively, were included, with 1 to 6 bacterial strains found in each peritoneal sample. During the first period, 80% of the biota was composed of enterobacteriacae and anaerobes and then decreased to 65%, whereas streptococci levels increased from 0 to 22%. Pansusceptibility rates remained stable (17%, 16.8%, and 15.6% for the 3 periods, respectively). Piperacillin, vancomycin, ticarcillin-clavulanic acid, and fluoroquinolones were associated with increased resistance rates, unlike antibiotic associations currently used as postoperative treatments. CONCLUSION: No significant increase in resistance rates of bacteriacae in complicated appendicitis in children was found over the last 20 years. Empirical antibiotherapy protocols currently recommended remain efficient on this particular biota.
Subject(s)
Anti-Bacterial Agents/therapeutic use , Appendicitis/microbiology , Drug Resistance, Bacterial , Gram-Negative Bacterial Infections/microbiology , Gram-Positive Bacterial Infections/microbiology , Adolescent , Anti-Bacterial Agents/economics , Appendectomy , Appendicitis/drug therapy , Appendicitis/economics , Appendicitis/surgery , Child , Child, Preschool , Combined Modality Therapy , Cost-Benefit Analysis , Disk Diffusion Antimicrobial Tests , Female , France , Gram-Negative Bacterial Infections/drug therapy , Gram-Negative Bacterial Infections/economics , Gram-Negative Bacterial Infections/surgery , Gram-Positive Bacterial Infections/drug therapy , Gram-Positive Bacterial Infections/economics , Gram-Positive Bacterial Infections/surgery , Humans , Male , Peritoneum/microbiology , Retrospective StudiesABSTRACT
We examined 785 placentas, including 51 from documented cases of congenital toxoplasmosis. Toxoplasma was detected in 16 placentas, including 1 in which congenital toxoplasmosis was ruled out. Placental screening had poor sensitivity (25%) but good specificity (99%), positive predictive value (93%), and negative predictive value (95%).
