Trend of Changes in Chloramphenicol Resistance during the Years 2017-2020: A Retrospective Report from Israel.

Resistant bacteria limit treatment options. This challenge has awakened interest in antibiotics that are no longer in use due to side effects, such as chloramphenicol. This work investigated trends in chloramphenicol resistance rates during 2017-2020 in bacteria isolated from diverse clinical samples at the Baruch Padeh Medical Center, Poriya, Israel. Bacteria were isolated from 3873 samples and identified using routine methods, including matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) technology. Chloramphenicol susceptibility was tested using a VITEK II instrument or by the Kirby-Bauer disk-diffusion test. The average chloramphenicol resistance rate was 24%, with no significant differences between study years. Chloramphenicol resistance was associated with sample origin (p < 0.001); isolates originating from sputum samples showed 49.8% resistance rate, compared to 2.3% of the body fluid isolates, 10.4% of the ear/eye isolates and 22.5% of the blood isolates. Furthermore, there was a significant decrease in chloramphenicol resistance among blood and ear/eye isolates during the study period (p = 0.01, p < 0.001, respectively). The highest resistance rate was among Pseudomonas aeruginosa isolates (50.5%). In conclusion, since chloramphenicol susceptibility seems to be retained, its comeback to the clinical world should be considered.

Personality and satisfaction with online courses: The relation between the Big Five personality traits and satisfaction with online learning activities.

Online courses have become widespread in higher education. Yet, despite their prevalence, they may not suit all learners. Personality influences learner satisfaction and therefore affects learning experience. This study explores the relation between personality traits (using Costa & McCare's Big-Five model) and student satisfaction with various of learning activities offered in online courses, called Techno-Pedagogical Learning Solutions (TPLS). The tested TPLS were discussion groups, digital books, online assignments, surveys/polls and media. Questionnaires were used to measure personality types and satisfaction of 108 university students enrolled in a credited online academic course. Significant correlations were found between all five personality traits and satisfaction with several TPLS. Cluster analysis method was applied to identify learners with similar personality traits. Four groups were formed and group's satisfaction score was measured. It was found that learners assigned to the "neurotic" group exhibited low satisfaction with all TPLS, contrary to learners assigned to the "non-neurotic" group. The findings clearly indicate that personality plays a significant role in online learner satisfaction. Thus, personality traits should be considered when designing learning activities for online courses. Such personality-based personalization may ensure that no learner is left behind, regardless of his\ her attitude toward online learning.

Equity factors during the COVID-19 pandemic: Difficulties in emergency remote teaching (ert) through online learning.

Since the onset of the COVID-19 pandemic, Emergency Remote Teaching through Online Learning (ERT-OL) has become the prevalent form of learning at many universities worldwide. At the same time, voices around the world have pointed to difficulties in online learning in general and to concerns regarding educational equity in particular. The current study sought to increase knowledge about specific hindering elements in ERT-OL and about the relationships between these elements from the standpoint of the following equity factors-socioeconomic status, language, and juggling among students who are also parents or working. To this end, the study analyzed 154 open-ended textual statements concerning the difficulties perceived by students at a university in Israel. The qualitative thematic analysis generated a map of hindering elements categorized in terms of a) processes: technology, pedagogy, content, situation and individual characteristics, and b) outcomes: cognitive, affective, social, and physical. The map revealed a mesh of intricate mediating and moderating links whose effect can intensify for each equity factor. On the positive side, seeds of mitigating strategies emerged as well. The study advances knowledge regarding ERT-OL hindering elements and their relationships and provides a better understanding of how these debilitating relationships may be exacerbated when equity factors are considered. Researchers and teachers interested in ERT-OL or in "normal" online learning in the future can use the map as a research and teaching framework to identify inequities and prevent further gaps.

Is Shunt Evaluation Useful in Children With Intraventricular Shunts With Seizures?

BACKGROUND: Seizures are a common presenting symptom to the emergency department (ED) in children with intraventricular shunts (IVS). The incidence of shunt malfunction and the yield of imaging studies in children with IVS presenting with seizures is unknown. OBJECTIVES: We assessed the utility and diagnostic yield of evaluation for shunt malfunction in patients with IVS with seizure and identified clinical predictors for shunt malfunction in these children. METHODS: We performed a retrospective review of children aged zero to 21 years of age with IVS who presented to the ED with seizure between 2011 and 2015. Demographic, clinical, laboratory and radiological data were collected. Shunt malfunction was diagnosed based on whether a shunt revision was performed during the hospitalization. RESULTS: We evaluated 408 ED visits (median age: six years [IQR: 3, 11], males 61.5%) for IVS with seizures. Few visits were for first seizure (37; 9.1%) or for status epilepticus (46 visits; 11.3%). Computerized tomography head was performed in 318 patients (95.2%), of which 32 scans (10.6%) were suggestive of shunt malfunction. A shunt series was performed in 302 (90.4%) and was suggestive of shunt malfunction in eight (2.6%) patients. Shunt malfunction was diagnosed in 40 of 408 visits (9.8%). Only a history of multiple (≥3) shunt revisions was significantly associated with shunt malfunction. There was no association between number, type, or prior history of seizures and shunt malfunction. CONCLUSION: Our study demonstrates a low yield of imaging studies for the evaluation of shunt malfunction in children with IVS with seizures, and shunt malfunction is an uncommon cause of seizures in these children.

Expression of full-length and truncated Fyn tyrosine kinase transcripts and encoded proteins during spermatogenesis and localization during acrosome biogenesis and fertilization.

We report that full-length and truncated transcripts of Fyn tyrosine protein kinase are expressed during testicular development. Truncated Fyn (tr-Fyn) transcripts encode a 24 kDa protein with a N-terminal (NT) domain, a complete Src homology (SH) 3 domain and an incomplete SH2 domain. The kinase domain is missing in tr-Fyn. In contrast, full-length Fyn transcripts encode a 59-55 kDa protein. Fractionated spermatids by centrifugal elutriation express tr-Fyn transcripts and protein, but not full-length Fyn transcripts and protein. Neither full-length Fyn nor tr-Fyn transcripts and encoded proteins are detected in elutriated pachytene spermatocytes. Sertoli cells express full-length and truncated Fyn throughout testicular development. In contrast, sperm contain full-length Fyn transcripts and protein but not the truncated form. tr-Fyn protein is visualized at the cytosolic side of Golgi membranes, derived proacrosomal vesicles, along the outer acrosome membrane and the inner acrosome membrane-acroplaxome complex anchoring the acrosome to the spermatid nuclear envelope. Fyn and phosphotyrosine immunoreactivity coexist in the tail of capacitated sperm. During fertilization, the Fyn-containing acroplaxome seen in the egg-bound and egg-fused sperm is no longer detected upon decondensation of the sperm nucleus. tr-Fyn expands the catalog of truncated tyrosine protein kinases expressed during spermiogenesis. We suggest that the NT and SH3 domains of tr-Fyn may recruit adaptor and effector proteins, in particular GTPase activating proteins, required for acrosome-acroplaxome biogenesis, acroplaxome F-actin dynamics and Sertoli cell function. During fertilization, full-length Fyn in the acroplaxome may contribute to a transient local signaling burst during the early events of sperm-egg interaction.

Signalling in mammalian egg activation: role of protein kinases.

Embryonic development is initiated after the fertilizing spermatozoon enters the egg and triggers a process known as 'egg activation'. Activation results in an increase in intracellular calcium concentration, cortical granule exocytosis (CGE), cell cycle resumption and recruitment of maternal mRNA. Various treatments can induce parthenogenetic activation characterized by the same manifestations. Signal transduction pathways similar to those known for somatic cells mediate the mammalian egg activation. This review focuses on the signal transduction pathways that occur during mammalian fertilization and during parthenogenetic egg activation. We discuss the possibility that members of the protein tyrosine kinase (PTKs) families, the Src family PTKs in particular, operate during egg activation and that protein kinase C can induce CGE.

Signaling through protein kinases during egg activation.

Following penetration of the zona pellucida, the mammalian spermatozoon binds and fuses with the egg plasma membrane, thereby fertilizing the egg that is still arrested at the second metaphase. Fertilization initiates in the egg a sequence of events referred to as 'egg activation'. An initial increase in intracellular Ca(2+) concentration ([Ca(2+)](i)) appears to be the very early cellular event observed which leads to the cortical granules exocytosis and resumption of meiosis. Various treatments can induce parthenogenetic activation mimicking at least part of the fertilization events. Similar to somatic cells, studies in mammalian eggs suggest that signal transduction pathways mediate egg activation. The initial increase in [Ca(2+)](i) appears to be critical for egg activation. However, other messengers such as protein kinase C (PKC) and protein tyrosine kinases (PTKs), were suggested as possible inducers of some aspects of egg activation. In the present work, studies concerning the involvement of protein kinases during egg activation in our laboratory and in others are summarized.