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1.
PLoS One ; 12(5): e0178176, 2017.
Article in English | MEDLINE | ID: mdl-28542446

ABSTRACT

The effect of neighbouring plants on crop root system architecture may directly interfere with water and nutrient acquisition, yet this important and interesting aspect of competition remains poorly understood. Here, the effect of the weed blackgrass (Alopecurus myosuroides Huds.) on wheat (Triticum aestivum L.) roots was tested, since a low density of this species (25 plants m-2) can lead to a 10% decrease in wheat yield and herbicide resistance is problematic. We used a simplified growth system based on gelled medium, to grow wheat alongside a neighbour, either another wheat plant, a blackgrass or Brachypodium dystachion individual (a model grass). A detailed analysis of wheat seminal root system architecture showed that the presence of a neighbour principally affected the root length, rather than number or diameter under a high nutrient regime. In particular, the length of first order lateral roots decreased significantly in the presence of blackgrass and Brachypodium. However, this effect was not noted when wheat plants were grown in low nutrient conditions. This suggests that wheat may be less sensitive to the presence of blackgrass when grown in low nutrient conditions. In addition, nutrient availability to the neighbour did not modulate the neighbour effect on wheat root architecture.


Subject(s)
Brachypodium/growth & development , Poaceae/growth & development , Triticum/growth & development , Biomass , Ecosystem , Models, Biological , Plant Roots/anatomy & histology , Plant Roots/growth & development , Triticum/anatomy & histology
2.
Proc Natl Acad Sci U S A ; 113(31): 8801-6, 2016 08 02.
Article in English | MEDLINE | ID: mdl-27432976

ABSTRACT

Hypoxic pulmonary vasoconstriction is correlated with pulmonary vascular remodeling. The hypoxia-inducible transcription factors (HIFs) HIF-1α and HIF-2α are known to contribute to the process of hypoxic pulmonary vascular remodeling; however, the specific role of pulmonary endothelial HIF expression in this process, and in the physiological process of vasoconstriction in response to hypoxia, remains unclear. Here we show that pulmonary endothelial HIF-2α is a critical regulator of hypoxia-induced pulmonary arterial hypertension. The rise in right ventricular systolic pressure (RVSP) normally observed following chronic hypoxic exposure was absent in mice with pulmonary endothelial HIF-2α deletion. The RVSP of mice lacking HIF-2α in pulmonary endothelium after exposure to hypoxia was not significantly different from normoxic WT mice and much lower than the RVSP values seen in WT littermate controls and mice with pulmonary endothelial deletion of HIF-1α exposed to hypoxia. Endothelial HIF-2α deletion also protected mice from hypoxia remodeling. Pulmonary endothelial deletion of arginase-1, a downstream target of HIF-2α, likewise attenuated many of the pathophysiological symptoms associated with hypoxic pulmonary hypertension. We propose a mechanism whereby chronic hypoxia enhances HIF-2α stability, which causes increased arginase expression and dysregulates normal vascular NO homeostasis. These data offer new insight into the role of pulmonary endothelial HIF-2α in regulating the pulmonary vascular response to hypoxia.


Subject(s)
Arginase/metabolism , Basic Helix-Loop-Helix Transcription Factors/metabolism , Endothelium, Vascular/metabolism , Hypertension, Pulmonary/metabolism , Animals , Arginase/genetics , Basic Helix-Loop-Helix Transcription Factors/genetics , Cell Hypoxia , Cells, Cultured , Endothelium, Vascular/cytology , Humans , Hypertension, Pulmonary/genetics , Hypoxia , Hypoxia-Inducible Factor 1, alpha Subunit/genetics , Hypoxia-Inducible Factor 1, alpha Subunit/metabolism , Male , Mice, Inbred C57BL , Mice, Knockout , Mice, Transgenic , Nitric Oxide/metabolism , Ventricular Function, Right/genetics , Ventricular Function, Right/physiology , Ventricular Pressure/genetics , Ventricular Pressure/physiology
3.
Plant Physiol ; 163(1): 253-62, 2013 Sep.
Article in English | MEDLINE | ID: mdl-23886625

ABSTRACT

Salinity (NaCl) stress impairs plant growth and inflicts severe crop losses. In roots, increasing extracellular NaCl causes Ca²âº influx to elevate cytosolic free Ca²âº ([Ca²âº](cyt)) as a second messenger for adaptive signaling. Amplification of the signal involves plasma membrane reduced nicotinamide adenine dinucleotide phosphate oxidase activation, with the resultant reactive oxygen species triggering Ca²âº influx. The genetic identities of the Ca²âº-permeable channels involved in generating the [Ca²âº](cyt) signal are unknown. Potential candidates in the model plant Arabidopsis (Arabidopsis thaliana) include annexin1 (AtANN1). Here, luminescent detection of [Ca²âº](cyt) showed that AtANN1 responds to high extracellular NaCl by mediating reactive oxygen species-activated Ca²âº influx across the plasma membrane of root epidermal protoplasts. Electrophysiological analysis revealed that root epidermal plasma membrane Ca²âº influx currents activated by NaCl are absent from the Atann1 loss-of-function mutant. Both adaptive signaling and salt-responsive production of secondary roots are impaired in the loss-of-function mutant, thus identifying AtANN1 as a key component of root cell adaptation to salinity.


Subject(s)
Annexins/physiology , Arabidopsis Proteins/physiology , Arabidopsis/metabolism , Calcium Signaling/genetics , Adaptation, Physiological/genetics , Annexins/genetics , Annexins/metabolism , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , Cell Membrane/metabolism , Plant Roots/genetics , Plant Roots/metabolism , Plant Roots/physiology , Salt Tolerance/genetics , Sodium Chloride/metabolism , Stress, Physiological
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