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1.
Cell ; 104(3): 409-20, 2001 Feb 09.
Article in English | MEDLINE | ID: mdl-11239398

ABSTRACT

When a kinase inactive form of Protein Kinase D (PKD-K618N) was expressed in HeLa cells, it localized to the trans-Golgi network (TGN) and caused extensive tubulation. Cargo that was destined for the plasma membrane was found in PKD-K618N-containing tubes but the tubes did not detach from the TGN. As a result, the transfer of cargo from TGN to the plasma membrane was inhibited. We have also demonstrated the formation and subsequent detachment of cargo-containing tubes from the TGN in cells stably expressing low levels of PKD-K618N. Our results suggest that PKD regulates the fission from the TGN of transport carriers that are en route to the cell surface.


Subject(s)
Cell Membrane/enzymology , Glycoproteins , Membrane Proteins , Protein Kinase C/physiology , trans-Golgi Network/metabolism , Biological Transport , CD4 Antigens/metabolism , Cell Line , Cell Membrane/metabolism , Clathrin/metabolism , Coatomer Protein/metabolism , Dose-Response Relationship, Drug , Endoplasmic Reticulum/metabolism , Endosomes/metabolism , Fluorescent Antibody Technique , Glutathione Transferase/metabolism , HeLa Cells , Humans , Membrane Glycoproteins/metabolism , Phosphorylation , Precipitin Tests , Protein Kinase C/genetics , Protein Transport , Recombinant Proteins/metabolism , Temperature , Time Factors , Transfection , Viral Envelope Proteins/metabolism
2.
J Cell Biol ; 149(2): 331-9, 2000 Apr 17.
Article in English | MEDLINE | ID: mdl-10769026

ABSTRACT

Incubation of permeabilized cells with mitotic extracts results in extensive fragmentation of the pericentriolarly organized stacks of cisternae. The fragmented Golgi membranes are subsequently dispersed from the pericentriolar region. We have shown previously that this process requires the cytosolic protein mitogen-activated protein kinase kinase 1 (MEK1). Extracellular signal-regulated kinase (ERK) 1 and ERK2, the known downstream targets of MEK1, are not required for this fragmentation (Acharya et al. 1998). We now provide evidence that MEK1 is specifically phosphorylated during mitosis. The mitotically phosphorylated MEK1, upon partial proteolysis with trypsin, generates a different peptide population compared with interphase MEK1. MEK1 cleaved with the lethal factor of the anthrax toxin can still be activated by its upstream mitotic kinases, and this form is fully active in the Golgi fragmentation process. We believe that the mitotic phosphorylation induces a change in the conformation of MEK1 and that this form of MEK1 recognizes Golgi membranes as a target compartment. Immunoelectron microscopy analysis reveals that treatment of permeabilized normal rat kidney (NRK) cells with mitotic extracts, treated with or without lethal factor, converts stacks of pericentriolar Golgi membranes into smaller fragments composed predominantly of tubuloreticular elements. These fragments are similar in distribution, morphology, and size to the fragments observed in the prometaphase/metaphase stage of the cell cycle in vivo.


Subject(s)
Antigens, Bacterial , Golgi Apparatus/physiology , Golgi Apparatus/ultrastructure , Mitogen-Activated Protein Kinase Kinases/metabolism , Mitosis/physiology , Protein Serine-Threonine Kinases/metabolism , Animals , Bacterial Toxins/pharmacology , CDC2 Protein Kinase/metabolism , Cell Line , Enzyme Activation , Golgi Apparatus/drug effects , Interphase , Intracellular Membranes/physiology , Intracellular Membranes/ultrastructure , MAP Kinase Kinase 1 , Mitogen-Activated Protein Kinase Kinases/chemistry , Phosphorylation , Protein Serine-Threonine Kinases/chemistry , Rats , Recombinant Proteins/metabolism , Signal Transduction , Trypsin
3.
Nature ; 402(6760): 429-33, 1999 Nov 25.
Article in English | MEDLINE | ID: mdl-10586885

ABSTRACT

Membrane fission is essential in intracellular transport. Acyl-coenzyme As (acyl-CoAs) are important in lipid remodelling and are required for fission of COPI-coated vesicles. Here we show that CtBP/BARS, a protein that functions in the dynamics of Golgi tubules, is an essential component of the fission machinery operating at Golgi tubular networks, including Golgi compartments involved in protein transport and sorting. CtBP/BARS-induced fission was preceded by the formation of constricted sites in Golgi tubules, whose extreme curvature is likely to involve local changes in the membrane lipid composition. We find that CtBP/BARS uses acyl-CoA to selectively catalyse the acylation of lysophosphatidic acid to phosphatidic acid both in pure lipidic systems and in Golgi membranes, and that this reaction is essential for fission. Our results indicate a key role for lipid metabolic pathways in membrane fission.


Subject(s)
Carrier Proteins/metabolism , Golgi Apparatus/metabolism , Intracellular Membranes/metabolism , Lysophospholipids/metabolism , Transcription Factors , Acyl Coenzyme A/metabolism , Acylation , Animals , Brain/metabolism , Brain/ultrastructure , Golgi Apparatus/ultrastructure , In Vitro Techniques , Intracellular Membranes/ultrastructure , Membrane Lipids/metabolism , Rats , Recombinant Proteins/metabolism
4.
J Biol Chem ; 274(25): 17705-10, 1999 Jun 18.
Article in English | MEDLINE | ID: mdl-10364211

ABSTRACT

Brefeldin A (BFA) is a fungal metabolite that disassembles the Golgi apparatus into tubular networks and causes the dissociation of coatomer proteins from Golgi membranes. We have previously shown that an additional effect of BFA is to stimulate the ADP-ribosylation of two cytosolic proteins of 38 and 50 kDa (brefeldin A-ADP-riboslyated substrate (BARS)) and that this effect greatly facilitates the Golgi-disassembling activity of the toxin. In this study, BARS has been purified from rat brain cytosol and microsequenced, and the BARS cDNA has been cloned. BARS shares high homology with two known proteins, C-terminal-binding protein 1 (CtBP1) and CtBP2. It is therefore a third member of the CtBP family. The role of BARS in Golgi disassembly by BFA was verified in permeabilized cells. In the presence of dialyzed cytosol that had been previously depleted of BARS or treated with an anti-BARS antibody, BFA potently disassembled the Golgi. However, in cytosol complemented with purified BARS, or even in control cytosols containing physiological levels of BARS, the action of BFA on Golgi disassembly was strongly inhibited. These results suggest that BARS exerts a negative control on Golgi tubulation, with important consequences for the structure and function of the Golgi complex.


Subject(s)
Brefeldin A/pharmacology , Carrier Proteins/genetics , GTP-Binding Proteins/metabolism , Golgi Apparatus/drug effects , Transcription Factors , ADP-Ribosylation Factors , Alcohol Oxidoreductases , Amino Acid Sequence , Animals , Brain/metabolism , COS Cells , Carrier Proteins/chemistry , Cloning, Molecular , DNA-Binding Proteins/chemistry , Lung/metabolism , Molecular Sequence Data , Nerve Tissue Proteins/chemistry , Nerve Tissue Proteins/genetics , Phosphoproteins/chemistry , Rats , Rats, Sprague-Dawley , Sequence Alignment , Spleen/metabolism , Transfection , Tumor Cells, Cultured
5.
Mol Cell Biochem ; 193(1-2): 43-51, 1999 Mar.
Article in English | MEDLINE | ID: mdl-10331637

ABSTRACT

The fungal toxin brefeldin A (BFA) dissociates coat proteins from Golgi membranes, causes the rapid disassembly of the Golgi complex and potently stimulates the ADP-ribosylation of two cytosolic proteins of 38 and 50 kDa. These proteins have been identified as the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and a novel guanine nucleotide binding protein (BARS-50), respectively. The role of ADP-ribosylation in mediating the effects of BFA on the structure and function of the Golgi complex was analyzed by several approaches including the use of selective pharmacological blockers of the reaction and the use of ADP-ribosylated cytosol and/or enriched preparations of the BFA-induced ADP-ribosylation substrates, GAPDH and BARS-50. A series of blockers of the BFA-dependent ADP-ribosylation reaction identified in our laboratory inhibited the effects of BFA on Golgi morphology and, with similar potency, the ADP-ribosylation of BARS-50 and GAPDH. In permeabilized RBL cells, the BFA-dependent disassembly of the Golgi complex required NAD+ and cytosol. Cytosol that had been previously ADP-ribosylated (namely, it contained ADP-ribosylated GAPDH and BARS-50), was instead sufficient to sustain the Golgi disassembly induced by BFA. Taken together, these results indicate that an ADP-ribosylation reaction is part of the mechanism of action of BFA and it might intervene in the control of the structure and function of the Golgi complex.


Subject(s)
Adenosine Diphosphate Ribose/metabolism , Adenosine Diphosphate Ribose/physiology , Brefeldin A/pharmacology , Carrier Proteins/physiology , Angiotensin-Converting Enzyme Inhibitors/pharmacology , Animals , Brefeldin A/antagonists & inhibitors , Cytosol/metabolism , Dose-Response Relationship, Drug , Glyceraldehyde-3-Phosphate Dehydrogenases/pharmacology , Golgi Apparatus/physiology , Inhibitory Concentration 50 , Leukemia/metabolism , Microscopy, Fluorescence , NAD/pharmacology , Peptide Fragments/pharmacology , Protein Synthesis Inhibitors/pharmacology , Rats , Time Factors , Tissue Distribution , Tumor Cells, Cultured
6.
J Biol Chem ; 272(22): 14200-7, 1997 May 30.
Article in English | MEDLINE | ID: mdl-9162051

ABSTRACT

Brefeldin A, a toxin inhibitor of vesicular traffic, induces the selective mono-ADP-ribosylation of two cytosolic proteins, glyceraldehyde-3-phosphate dehydrogenase and the novel GTP-binding protein BARS-50. Here, we have used a new quantitative assay for the characterization of this reaction and the development of specific pharmacological inhibitors. Mono-ADP-ribosylation is activated by brefeldin A with an EC50 of 17.0 +/- 3.1 microg/ml, but not by biologically inactive analogs including a brefeldin A stereoisomer. Brefeldin A acts by increasing the Vmax of the reaction, whereas it does not influence the Km of the enzyme for NAD+ (154 +/- 13 microM). The enzyme is an integral membrane protein present in most tissues and is modulated by Zn2+, Cu2+, ATP (but not by other nucleotides), pH, temperature, and ionic strength. To identify inhibitors of the reaction, a large number of drugs previously tested as blockers of bacterial ADP-ribosyltransferases were screened. Two classes of molecules, one belonging to the coumarin group (dicumarol, coumermycin A1, and novobiocin) and the other to the quinone group (ilimaquinone, benzoquinone, and naphthoquinone), rather potently and specifically inhibited brefeldin A-dependent mono-ADP-ribosylation. When tested in living cells, these molecules antagonized the tubular reticular redistribution of the Golgi complex caused by brefeldin A at concentrations similar to those active in the mono-ADP-ribosylation assay in vitro, suggesting a role for mono-ADP-ribosylation in the cellular actions of brefeldin A.


Subject(s)
Cyclopentanes/pharmacology , GTP-Binding Proteins/metabolism , Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism , Protein Processing, Post-Translational/drug effects , Protein Synthesis Inhibitors/pharmacology , Adenosine Diphosphate , Animals , Brefeldin A , Cell Line , Male , Rats , Rats, Sprague-Dawley , Ribose , Structure-Activity Relationship , Tissue Distribution
7.
Adv Exp Med Biol ; 419: 331-5, 1997.
Article in English | MEDLINE | ID: mdl-9193673

ABSTRACT

Brefeldin A (BFA) is a fungal metabolite that exerts generally inhibitory actions on membrane transport and induces the disappearance of the Golgi complex. Previously we have shown that BFA stimulates the ADP-ribosylation of two cytosolic proteins of 38 and 50 KD. The BFA-binding components mediating the BFA-sensitive ADP-ribosylation (BAR) and the effect of BFA on ARF binding to Golgi membranes have similar specificities and affinities for BFA and its analogues, suggesting that BAR may have a role in the cellular effects of BFA. To investigate this we used the approach to impair BAR activity by the use of BAR inhibitors. A series of BAR inhibitors was developed and their effects were studied in RBL cells treated with BFA. In addition to the common ADP-ribosylation inhibitors (nicotinamide and aminobenzamide), compounds belonging to the cumarin (novobiocin, cumermycin, dicumarol) class were active BAR inhibitors. All BAR inhibitors were able to prevent the BFA-induced redistribution of a Golgi marker (Helix pomatia lectin) into the endoplasmic reticulum, as assessed in immunofluorescence experiments. At the ultrastructural level, BAR inhibitors prevented the tubular-vesicular transformation of the Golgi complex caused by BFA. The potencies of these compounds in preventing the BFA effects on the Golgi complex were similar to those at which they inhibited BAR. Altogether these data support the hypothesis that BAR mediates at least some of the effects of BFA on the Golgi structure and function.


Subject(s)
Adenosine Diphosphate Ribose/metabolism , Cyclopentanes/pharmacology , Golgi Apparatus/drug effects , Brefeldin A , Golgi Apparatus/physiology , Golgi Apparatus/ultrastructure
8.
Adv Exp Med Biol ; 419: 337-42, 1997.
Article in English | MEDLINE | ID: mdl-9193674

ABSTRACT

We have recently described a novel enzymatic mono-ADP-ribosyl transfer reaction induced by brefeldin A, a well characterized inhibitor of vesicular traffic, which selectively modifies two cytosolic proteins of 38 kDa (p38) and 50 kDa (BARS-50). p38 was identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme and a multifunctional protein involved in several cellular processes; BARS-50 might be a novel G protein, since it is able to bind GTP and the beta gamma subunit of G proteins. We have characterized this enzymatic activity and screened in vitro the effects of different drugs belonging to the coumarine (dicumarol, coumermicin A1 and novobiocin) and quinone (ilimaquinones, benzoquinones and naphtoquinones) class. These drugs blocked the BFA-dependent mono-ADP-ribosylation, showed remarkable effects on Golgi morphology in control cells, and antagonized the tubular reticular redistribution of the Golgi complex in brefeldin A treated cells (see papers of Corda and Colanzi in this issue) suggesting a possible role for ADP-ribosylation in both the cellular effects of brefeldin A and the maintenance of the structure/function of the Golgi complex.


Subject(s)
Adenosine Diphosphate Ribose/metabolism , Cyclopentanes/pharmacology , GTP-Binding Proteins/metabolism , Brefeldin A , Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism
9.
J Cell Biol ; 139(5): 1109-18, 1997 Dec 01.
Article in English | MEDLINE | ID: mdl-9382860

ABSTRACT

We have investigated the role of the ADP- ribosylation induced by brefeldin A (BFA) in the mechanisms controlling the architecture of the Golgi complex. BFA causes the rapid disassembly of this organelle into a network of tubules, prevents the association of coatomer and other proteins to Golgi membranes, and stimulates the ADP-ribosylation of two cytosolic proteins of 38 and 50 kD (GAPDH and BARS-50; De Matteis, M.A., M. DiGirolamo, A. Colanzi, M. Pallas, G. Di Tullio, L.J. McDonald, J. Moss, G. Santini, S. Bannykh, D. Corda, and A. Luini. 1994. Proc. Natl. Acad. Sci. USA. 91:1114-1118; Di Girolamo, M., M.G. Silletta, M.A. De Matteis, A. Braca, A. Colanzi, D. Pawlak, M.M. Rasenick, A. Luini, and D. Corda. 1995. Proc. Natl. Acad. Sci. USA. 92:7065-7069). To study the role of ADP-ribosylation, this reaction was inhibited by depletion of NAD+ (the ADP-ribose donor) or by using selective pharmacological blockers in permeabilized cells. In NAD+-depleted cells and in the presence of dialized cytosol, BFA detached coat proteins from Golgi membranes with normal potency but failed to alter the organelle's structure. Readdition of NAD+ triggered Golgi disassembly by BFA. This effect of NAD+ was mimicked by the use of pre-ADP- ribosylated cytosol. The further addition of extracts enriched in native BARS-50 abolished the ability of ADP-ribosylated cytosol to support the effect of BFA. Pharmacological blockers of the BFA-dependent ADP-ribosylation (Weigert, R., A. Colanzi, A. Mironov, R. Buccione, C. Cericola, M.G. Sciulli, G. Santini, S. Flati, A. Fusella, J. Donaldson, M. DiGirolamo, D. Corda, M.A. De Matteis, and A. Luini. 1997. J. Biol. Chem. 272:14200-14207) prevented Golgi disassembly by BFA in permeabilized cells. These inhibitors became inactive in the presence of pre-ADP-ribosylated cytosol, and their activity was rescued by supplementing the cytosol with a native BARS-50-enriched fraction. These results indicate that ADP-ribosylation plays a role in the Golgi disassembling activity of BFA, and suggest that the ADP-ribosylated substrates are components of the machinery controlling the structure of the Golgi apparatus.


Subject(s)
Adenosine Diphosphate Ribose/metabolism , Cyclopentanes/pharmacology , Golgi Apparatus/ultrastructure , NAD/metabolism , Animals , Brefeldin A , Cell Membrane Permeability , Coatomer Protein , Endoplasmic Reticulum/enzymology , Golgi Apparatus/drug effects , Golgi Apparatus/enzymology , Membrane Proteins/metabolism , Rats , Tumor Cells, Cultured
10.
Proc Natl Acad Sci U S A ; 92(15): 7065-9, 1995 Jul 18.
Article in English | MEDLINE | ID: mdl-7624370

ABSTRACT

Brefeldin A, a fungal metabolite that inhibits membrane transport, induces the mono(ADP-ribosyl)ation of two cytosolic proteins of 38 and 50 kDa as judged by SDS/PAGE. The 38-kDa substrate has been previously identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We report that the 50-kDa BFA-induced ADP-ribosylated substrate (BARS-50) has native forms of 170 and 130 kDa, as determined by gel filtration of rat brain cytosol, indicating that BARS-50 might exist as a multimeric complex. BARS-50 can bind GTP, as indicated by blot-overlay studies with [alpha-32P]GTP and by photoaffinity labeling with guanosine 5'-[gamma-32P] [beta,gamma-(4-azidoanilido)]triphosphate. Moreover, ADP-ribosylation of BARS-50 was completely inhibited by the beta gamma subunit complex of G proteins, while the ADP-ribosylation of GAPDH was unmodified, indicating that this effect was due to an interaction of the beta gamma complex with BARS-50, rather than with the ADP-ribosylating enzyme. Two-dimensional gel electrophoresis and immunoblot analysis shows that BARS-50 is a group of closely related proteins that appear to be different from all the known GTP-binding proteins.


Subject(s)
Adenosine Diphosphate Ribose/biosynthesis , Cyclopentanes/metabolism , GTP-Binding Proteins/metabolism , Guanosine Triphosphate/metabolism , Affinity Labels , Animals , Brain/metabolism , Brefeldin A , Cattle , Cells, Cultured , Cytosol/metabolism , Electrophoresis, Gel, Two-Dimensional , Guanine Nucleotides/metabolism , Membrane Proteins/metabolism , Protein Binding , Protein Conformation , Protein Processing, Post-Translational , Rats , Thyroid Gland/cytology , Thyroid Gland/metabolism
11.
Proc Natl Acad Sci U S A ; 91(3): 1114-8, 1994 Feb 01.
Article in English | MEDLINE | ID: mdl-8302839

ABSTRACT

Brefeldin A (BFA) is a fungal metabolite that exerts profound and generally inhibitory actions on membrane transport. At least some of the BFA effects are due to inhibition of the GDP-GTP exchange on the ADP-ribosylation factor (ARF) catalyzed by membrane protein(s). ARF activation is likely to be a key event in the association of non-clathrin coat components, including ARF itself, onto transport organelles. ARF, in addition to participating in membrane transport, is known to function as a cofactor in the enzymatic activity of cholera toxin, a bacterial ADP-ribosyltransferase. In this study we have examined whether BFA, in addition to inhibiting membrane transport, might affect endogenous ADP-ribosylation in eukaryotic cells. Two cytosolic proteins of 38 and 50 kDa were enzymatically ADP-ribosylated in the presence of BFA in cellular extracts. The 38-kDa substrate was tentatively identified as the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase. The BFA-binding components mediating inhibition of membrane traffic and stimulation of ADP-ribosylation appear to have the same ligand specificity. These data demonstrate the existence of a BFA-sensitive mono(ADP-ribosyl)transferase that may play a role in membrane movements.


Subject(s)
Adenosine Diphosphate Ribose/metabolism , Cyclopentanes/pharmacology , GTP-Binding Proteins/metabolism , Mycotoxins/pharmacology , ADP Ribose Transferases , ADP-Ribosylation Factors , Animals , Biological Transport, Active , Brefeldin A , Cell Line , Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism , NAD/metabolism , Poly(ADP-ribose) Polymerases/metabolism , Proteins/metabolism , Rats , Substrate Specificity
12.
Farmaco ; 45(6 Suppl): 751-5, 1990 Jun.
Article in English | MEDLINE | ID: mdl-2400525

ABSTRACT

A method for the determination of 2,6-dimethyl-4-(2'-nitrosophenyl)-3,5-pyridinedicarboxylic acid dimethylester in nifedipine (bulk material and pharmaceutical formulations) by second-derivative ultraviolet spectrophotometry is described. The procedure is simple and rapid and gives accurate and precise results.


Subject(s)
Dihydropyridines/analysis , Nifedipine/analysis , Indicators and Reagents , Solutions , Spectrophotometry, Ultraviolet , Tablets
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