ABSTRACT
We used native polyacrylamide gel electrophoresis to identify polymorphism levels in α- and ß-esterase loci from leaf tissues of Brazilian soybean cultivars for the analysis of population genetic diversity and structure, and to investigate relationships between conventional and genetically modified cultivars. The cultivars included lines developed by a soybean-grower cooperative (CD), by EMBRAPA (BR), and "Roundup Ready" (RR) cultivars. Esterase isozymes recorded with α-naphthyl acetate and ß-naphthyl acetate were produced from 14 loci. Two to three allelic variants were detected in leaves from 420 plants of 21 CD, BR, and RR cultivars at Est-1, Est-2, Est-3, Est-5, and Est-14 loci. The estimated proportion of polymorphic loci in CD cultivars was 21.4%, and in BR and RR cultivars it was 28.6%. High and low H(O) and H(E) values were observed within CD and BR cultivars and a very high cultivar differentiation level was evident in the plants of the 21 CD, BR, and RR cultivars (F(ST) = 0.3865). A low level of differentiation (F(ST) = 0.0289) was detected between conventional and RR cultivars. Plants from cultivar BR37 had the highest level of genetic differentiation compared to the other cultivars. The genetic basis of BR cultivars (0.5538-0.9748) was found to be broader than the genetic basis of CD cultivars (0.7058 for CD205 and CD209 and 0.9995 for CD205 and CD208). Higher genetic identity was detected between plants of CD and CDRR cultivars (I = 0.9816). Understanding the genetic structure of these populations can help provide specific culture strategies for each cultivar, depending on its level of heterozygosity.
Subject(s)
Esterases/genetics , Genetic Loci , Glycine max/genetics , Plant Leaves/genetics , Polymorphism, Genetic , Brazil , Plant Leaves/enzymology , Glycine max/enzymologyABSTRACT
Random amplified polymorphic DNA (RAPD) markers were used to detect polymorphism and to examine relationships among four table grape clones from northwestern Paraná, in southern Brazil. The 10 primers used for RAPD fingerprints generated 126 reproducible fragments, of which 63, 68, 76, and 72 were polymorphic in cultivars Italia, Rubi, Benitaka, and Brasil, respectively. Among the primers, OPP-08 generated the highest number of fragments, whereas OPE-15 was the most efficient for discriminating polymorphic fragments. The distribution of the clones by cluster analysis indicated that there were no differences in RAPD markers between the colored mutant and the original clone (cultivar Italia), supporting the hypothesis that the non-colored and the colored mutant are the same cultivar. However, we found high levels of polymorphism within and between the cultivars Italia, Rubi, Benitaka, and Brasil (65.1%), contrary to a previous hypothesis that the four clones are genetically uniform. This confirmed our expectation of genetic variation among the clones and within each clone. We conclude that the primers are useful for analyzing the development of the genetic diversity within each of these clones.
