1.
Anim Reprod Sci
; 100(3-4): 402-10, 2007 Aug.
Article
in English
| MEDLINE
| ID: mdl-17118586
ABSTRACT
An efficient protocol was developed to synchronize and superovulate mature pigs for the collection of pronuclear stage embryos suitable for DNA microinjection. A timed and coordinated regimen of Lutalyse, PG600 and Chorulon along with daily checking for estrus allowed synchronization of groups of gilts having estrous cycles at regular intervals. Pigs 10-16 days after the beginning of standing estrus have been successfully synchronized into estrus using this protocol. A standard dose of each drug was used independent of size or age of the animal. One protocol averaged 38.9 ovulations and 31.1 one-cell embryos recovered per animal.
Subject(s)
Estrus Synchronization/methods , Superovulation/physiology , Swine/physiology , Animals , Chorionic Gonadotropin/pharmacology , Dinoprost/analogs & derivatives , Dinoprost/pharmacology , Embryo, Mammalian , Estrous Cycle , Female , Superovulation/drug effects
2.
Lab Anim (NY)
; 31(1): 25-31, 2002 Jan.
Article
in English
| MEDLINE
| ID: mdl-11910396