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1.
Pest Manag Sci ; 80(5): 2267-2281, 2024 May.
Article in English | MEDLINE | ID: mdl-36827249

ABSTRACT

BACKGROUND: Cabbage stem flea beetle (CSFB; Psylliodes chrysocephala L.) management in oilseed rape (Brassica napus L.) has become an urgent issue in the absence of permitted and effective insecticides. Understanding the meteorological and management factors affecting their population dynamics has become critical to the development of pest management strategies. RESULTS: The spatio-temporal changes in CSFB larval populations were assessed both in autumn and spring, in the UK from 2003 to 2017 (a period encompassing pre-and post-neonicotinoid insecticide restriction). After the neonicotinoid ban in 2013, the number of larvae both in autumn and spring increased 10-fold in the UK. When neonicotinoids were available, later sown crops contained fewer larvae than early sown crops, and bigger fields had fewer larvae than smaller fields, whereas after the ban, bigger fields tended to have more larvae than smaller fields. Wet and mild/hot Septembers were related with higher numbers of larvae when neonicotinoids were available and with lower larval numbers after the neonicotinoid ban. Low temperatures in December and January combined with high rainfall were related with high numbers of larvae in spring both before and after the neonicotinoid ban. CONCLUSION: This study will help to produce decision support systems that allow future predictions of regional CSFB population changes and will help growers and consultants to adjust their management methods to reduce the risk of high infestations. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.


Subject(s)
Brassica napus , Brassica , Coleoptera , Insecticides , Siphonaptera , Animals , Larva , Insecticides/pharmacology , Neonicotinoids/pharmacology , United Kingdom
2.
Pest Manag Sci ; 73(5): 967-975, 2017 May.
Article in English | MEDLINE | ID: mdl-27470297

ABSTRACT

BACKGROUND: An outbreak of EU-quarantine-listed pest Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambicidae), the Asian longhorn beetle, in Kent (UK) resulted in environmentally and financially costly eradication action being taken. In this study the potential of using multi-element stable isotope or trace element analyses to determine the geographical origin of individual specimens has been investigated. RESULTS: The isotope ratios of A. glabripennis individuals for hydrogen varied within and across five locations. Carbon isotope ratios fell within the expected values for C3 plants (trees using the photosynthetic pathway common for moderate climates). Nitrogen isotope ratios indicated separation of UK laboratory from US (New York, Ohio, Massachusetts) beetles, while sulphur isotope ratios distinguished beetles from New York against the other four locations. Three trace elements (TEs) separated UK laboratory-reared beetles from US beetles (Ohio and New York) with ∼ 68% confidence. CONCLUSIONS: Stable isotope and TE analyses show potential to differentiate between newly arrived A. glabripennis individuals and those from previously undetected in-country populations, which would be of immediate practical benefit in making appropriate strategic decisions on surveillance and eradication. Analyses of additional samples from (i) the same populations, (ii) different locations and (iii) a variety of host trees will enhance the overall picture. © 2016 Crown copyright. Pest Management Science © 2016 Society of Chemical Industry.


Subject(s)
Coleoptera/chemistry , Geography , Trace Elements/analysis , Animals , Coleoptera/classification , Hydrogen/chemistry , Isotopes/chemistry
3.
J Virol Methods ; 235: 119-124, 2016 09.
Article in English | MEDLINE | ID: mdl-27260658

ABSTRACT

A new Torradovirus tentatively named Carrot torrado virus (CaTV) was an incidental finding following a next generation sequencing study investigating internal vascular necrosis in carrot. The closest related viruses are Lettuce necrotic leaf curl virus (LNLCV) found in the Netherlands in 2011 and Motherwort yellow mottle virus (MYMoV) found in Korea in 2014. Primers for reverse transcriptase-PCR (RT-PCR) and RT-qPCR were designed with the aim of testing for the presence of virus in plant samples collected from the field. Both methods successfully amplified the target from infected samples but not from healthy control samples. The specificity of the CaTV assay was also checked against other known carrot viruses and no cross-reaction was seen. A comparative study between methods showed RT-qPCR was the most reliable method, giving positive results in samples where RT-PCR fails. Evaluation of the Ct values following RT-qPCR and a direct comparison demonstrated this was due to improved sensitivity. The previous published Torradovirus genus specific RT-PCR primers were tested and shown to detect CaTV. Also, virus transmission experiments carried out suggest that unlike other species of the same genus, Carrot torrado virus could be aphid-transmitted.


Subject(s)
Daucus carota/virology , Plant Diseases/virology , Plant Viruses/isolation & purification , RNA Viruses/isolation & purification , Animals , Aphids/virology , DNA Primers , High-Throughput Nucleotide Sequencing , Plant Leaves/virology , Plant Viruses/genetics , RNA Viruses/genetics , RNA, Viral/genetics , Reverse Transcriptase Polymerase Chain Reaction , Sensitivity and Specificity
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