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1.
Ocul Surf ; 22: 245-266, 2021 10.
Article in English | MEDLINE | ID: mdl-34520870

ABSTRACT

Aniridia, a rare congenital disease, is often characterized by a progressive, pronounced limbal insufficiency and ocular surface pathology termed aniridia-associated keratopathy (AAK). Due to the characteristics of AAK and its bilateral nature, clinical management is challenging and complicated by the multiple coexisting ocular and systemic morbidities in aniridia. Although it is primarily assumed that AAK originates from a congenital limbal stem cell deficiency, in recent years AAK and its pathogenesis has been questioned in the light of new evidence and a refined understanding of ocular development and the biology of limbal stem cells (LSCs) and their niche. Here, by consolidating and comparing the latest clinical and preclinical evidence, we discuss key unanswered questions regarding ocular developmental aspects crucial to AAK. We also highlight hypotheses on the potential role of LSCs and the ocular surface microenvironment in AAK. The insights thus gained lead to a greater appreciation for the role of developmental and cellular processes in the emergence of AAK. They also highlight areas for future research to enable a deeper understanding of aniridia, and thereby the potential to develop new treatments for this rare but blinding ocular surface disease.


Subject(s)
Aniridia , Corneal Diseases , Scleral Diseases , Cornea , Corneal Diseases/etiology , Humans , Stem Cells
2.
Neuroscience ; 271: 119-36, 2014 Jun 20.
Article in English | MEDLINE | ID: mdl-24785679

ABSTRACT

Mental and neurological illnesses affect one in four people. While genetic linkage analyses have shown an association of nuclear distribution factor E (NDE1, or NudE) and its ohnolog NDE-like 1 (NDEL1, or Nudel) with mental disorders, the cellular mechanisms remain unclear. In the present study, we have demonstrated that Nde1 and Ndel1 are differentially localised in the subventricular zone (SVZ) of the forebrain and the subgranular zone (SGZ) of the hippocampus, two regions where neurogenesis actively occurs in the adult brain. Nde1, but not Ndel1, is localized to putative SVZ stem cells, and to actively dividing progenitors of the SGZ. The influence of these proteins on neural stem cell differentiation was investigated by overexpression in a hippocampal neural stem cell line, HCN-A94. Increasing Nde1 expression in this neural stem cell line led to increased neuronal differentiation while decreasing levels of astroglial differentiation. In primary cultured neurons and astrocytes, Nde1 and Ndel1 were found to have different but comparable subcellular localizations. In addition, we have shown for the first time that Nde1 is heterogeneously distributed in cortical astrocytes of human brains. Our data indicate that Nde1 and Ndel1 have distinct but overlapping distribution patterns in mouse brain and cultured nerve cells. They may function differently and therefore their dosage changes may contribute to some aspects of mental disorders.


Subject(s)
Brain/metabolism , Carrier Proteins/metabolism , Cell Cycle Proteins/metabolism , Microtubule-Associated Proteins/metabolism , Neural Stem Cells/metabolism , Animals , Astrocytes/metabolism , Carrier Proteins/genetics , Cell Cycle Proteins/genetics , Cell Line , Cells, Cultured , Hippocampus/metabolism , Humans , Lateral Ventricles/metabolism , Male , Mice, Inbred C57BL , Microtubule-Associated Proteins/genetics , Middle Aged , Neurons/metabolism , Prosencephalon/metabolism , Rats
3.
J Pathol ; 215(4): 421-30, 2008 Aug.
Article in English | MEDLINE | ID: mdl-18491289

ABSTRACT

Heterozygosity for the transcription factor PAX6 causes eye disease in humans, characterized by corneal opacity. The molecular aetiology of such disease was investigated using a Pax6+/- mouse model. We found that the barrier function of uninjured Pax6+/- corneas was compromised and that Ca2+-PKC/PLC-ERK/p38 signalling pathways were abnormally activated, mimicking a 'wounded' epithelial state. Using proteomic analysis and direct assay for oxidized proteins, Pax6+/- corneas were found to be susceptible to oxidative stress and they exhibited a wound-healing delay which could be rescued by providing reducing agents such as glutathione. Pax6 protein was oxidized and excluded from the nucleus of stressed corneal epithelial cells, with concomitant loss of corneal epithelial markers and expression of fibroblast/myofibroblast markers. We suggest a chronic wound model for Pax6-related corneal diseases, in which oxidative stress underlies a positive feedback mechanism by depleting nuclear Pax6, delaying wound healing, and activating cell signalling pathways that lead to metaplasia of the corneal epithelium. The study mechanistically links a relatively minor dosage deficiency of a transcription factor with potentially catastrophic degenerative corneal disease.


Subject(s)
Aniridia/metabolism , Corneal Diseases/metabolism , Epithelium, Corneal/metabolism , Eye Proteins/metabolism , Homeodomain Proteins/metabolism , Paired Box Transcription Factors/metabolism , Repressor Proteins/metabolism , Animals , Chronic Disease , Extracellular Signal-Regulated MAP Kinases/analysis , Extracellular Signal-Regulated MAP Kinases/metabolism , Eye Proteins/analysis , Eye Proteins/genetics , Glutathione/pharmacology , Homeodomain Proteins/analysis , Homeodomain Proteins/genetics , Hydrogen Peroxide/pharmacology , Mice , Mice, Inbred CBA , Mice, Knockout , Microscopy, Electron, Scanning , Models, Animal , Oxidative Stress , PAX6 Transcription Factor , Paired Box Transcription Factors/analysis , Paired Box Transcription Factors/genetics , Repressor Proteins/analysis , Repressor Proteins/genetics , Signal Transduction , Wound Healing
4.
Proc Natl Acad Sci U S A ; 98(17): 9688-93, 2001 Aug 14.
Article in English | MEDLINE | ID: mdl-11481423

ABSTRACT

We describe lens defects in heterozygous small eye mice, and autonomous deficiencies of Pax6(+/-) cells in the developing lens of Pax6(+/+) <--> Pax6(+/-) chimeras. Two separate defects of the lens were identified by analyzing the distribution of heterozygous cells in chimeras: Pax6(+/-) cells are less readily incorporated into the lens placode than wild type, and those that are incorporated into the lens are not maintained efficiently in the proliferating lens epithelium. The lens of chimeric eyes is, therefore, predominantly wild type from embryonic day 16.5 onwards, whereas heterozygous cells contribute normally to all other eye tissues. Eye size and defects of the iris and cornea are corrected in fetal and adult chimeras with up to 80% mutant cells. Therefore, these aspects of the phenotype may be secondary consequences of primary defects in the lens, which has clinical relevance for the human aniridia (PAX6(+/-)) phenotype.


Subject(s)
Anterior Eye Segment/abnormalities , Eye Abnormalities/genetics , Eye Proteins/physiology , Homeodomain Proteins/physiology , Lens, Crystalline/abnormalities , Animals , Anterior Eye Segment/embryology , Cell Lineage , Chimera , Disease Models, Animal , Epithelial Cells/pathology , Eye Proteins/genetics , Heterozygote , Homeodomain Proteins/genetics , Lens, Crystalline/embryology , Mice , Mice, Mutant Strains , Morphogenesis/genetics , PAX6 Transcription Factor , Paired Box Transcription Factors , Repressor Proteins , Selection, Genetic
5.
J Cell Biol ; 150(3): 657-66, 2000 Aug 07.
Article in English | MEDLINE | ID: mdl-10931875

ABSTRACT

Two major isoforms of the cell adhesion molecule neurofascin NF186 and NF155 are expressed in the central nervous system (CNS). We have investigated their roles in the assembly of the node of Ranvier and show that they are targeted to distinct domains at the node. At the onset of myelination, NF186 is restricted to neurons, whereas NF155 localizes to oligodendrocytes, the myelin-forming glia of the CNS. Coincident with axon ensheathment, NF155 clusters at the paranodal regions of the myelin sheath where it localizes in apposition to the axonal adhesion molecule paranodin/contactin-associated protein (Caspr1), which is a constituent of the septate junction-like axo-glial adhesion zone. Immunoelectron microscopy confirmed that neurofascin is a glial component of the paranodal axo-glial junction. Concentration of NF155 with Caspr1 at the paranodal junctions of peripheral nerves is also a feature of Schwann cells. In Shiverer mutant mice, which assemble neither compact CNS myelin nor normal paranodes, NF155 (though largely retained at the cell body) is also distributed at ectopic sites along axons, where it colocalizes with Caspr1. Hence, NF155 is the first glial cell adhesion molecule to be identified in the paranodal axo-glial junction, where it likely interacts with axonal proteins in close association with Caspr1.


Subject(s)
Cell Adhesion Molecules/isolation & purification , Intercellular Junctions/physiology , Myelin Sheath/physiology , Nerve Growth Factors/isolation & purification , Neuroglia/physiology , Oligodendroglia/physiology , Animals , Coculture Techniques , Fluorescent Antibody Technique , Ganglia, Spinal/cytology , Membrane Glycoproteins/isolation & purification , Mice , Mice, Mutant Strains , Neuropeptides/isolation & purification , Optic Nerve/cytology , Pigment Epithelium of Eye/ultrastructure , Protein Isoforms/isolation & purification , Ranvier's Nodes/physiology , Rats , Rats, Sprague-Dawley , Schwann Cells/cytology , Sciatic Nerve/cytology , Sodium Channels/genetics
6.
Development ; 127(5): 945-56, 2000 Mar.
Article in English | MEDLINE | ID: mdl-10662634

ABSTRACT

Chimaeric mice were made by aggregating Pax6(-/-) and wild-type mouse embryos, in order to study the interaction between the optic vesicle and the prospective lens epithelium during early stages of eye development. Histological analysis of the distribution of homozygous mutant cells in the chimaeras showed that the cell-autonomous removal of Pax6(-/-) cells from the lens, shown previously at E12.5, is nearly complete by E9.5. Most mutant cells are eliminated from an area of facial epithelium wider than, but including, the developing lens placode. This result suggests a role for Pax6 in maintaining a region of the facial epithelium that has the tissue competence to undergo lens differentiation. Segregation of wild-type and Pax6(-/-) cells occurs in the optic vesicle at E9.5 and is most likely a result of different adhesive properties of wild-type and mutant cells. Also, proximo-distal specification of the optic vesicle (as assayed by the elimination of Pax6(-/-) cells distally), is disrupted in the presence of a high proportion of mutant cells. This suggests that Pax6 operates during the establishment of patterning along the proximo-distal axis of the vesicle. Examination of chimaeras with a high proportion of mutant cells showed that Pax6 is required in the optic vesicle for maintenance of contact with the overlying lens epithelium. This may explain why Pax6(-/-) optic vesicles are inefficient at inducing a lens placode. Contact is preferentially maintained when the lens epithelium is also wild-type. Together, these results demonstrate requirements for functional Pax6 in both the optic vesicle and surface epithelia in order to mediate the interactions between the two tissues during the earliest stages of eye development.


Subject(s)
DNA-Binding Proteins/physiology , Eye/embryology , Homeodomain Proteins , Morphogenesis/physiology , Animals , Chimera , Crosses, Genetic , DNA-Binding Proteins/deficiency , DNA-Binding Proteins/genetics , Epithelium/embryology , Eye/cytology , Eye Proteins/genetics , Eye Proteins/physiology , Face/embryology , Female , Genotype , Heterozygote , Lens, Crystalline/cytology , Lens, Crystalline/embryology , Male , Mice , Mice, Knockout , PAX6 Transcription Factor , Paired Box Transcription Factors , Pigment Epithelium of Eye/embryology , Repressor Proteins
7.
Mech Dev ; 71(1-2): 143-50, 1998 Feb.
Article in English | MEDLINE | ID: mdl-9507096

ABSTRACT

In an effort to define the roles of bone morphogenic proteins (BMPs) and fibroblast growth factors (FGFs) during chick limb development more closely, we have implanted beads impregnated with these growth factors into chick limb buds between stages 20 and 26. Embryos were sacrificed at the time the bone chondrocyte condensations first appear (stages 27-28). Implantation of beads containing BMPs at the earlier stages (20-22) caused apoptosis to occur, in the most severe cases leading to complete limb degeneration. Application of FGF4, either in the same, or in a different bead, prevented the BMP-induced apoptosis. We argue that the apoptosis observed on removal of the AER prior to stage 23 of development could be brought about by BMPs. The action of epithelial FGF in preventing BMP-mediated apoptosis in the mesenchyme would define a novel aspect of epithelial-mesenchymal interactions. Implanting the BMP4 beads into the core of the limb bud a day later (stages 25-26) caused intense chondrogenesis rather than apoptosis. FGF4 could again nullify this effect and by itself caused a reduction in bone size. This is the reverse of the functional relationship these growth factors have in mouse tooth specification (where it is BMP4 that inhibits the FGF8 function), and suggests that the balance between the effects of FGFs and BMPs could control the size of the chondrocyte precursor cell pool. In this way members of these two growth factor families could control the size of appendages when they are initially formed.


Subject(s)
Apoptosis/drug effects , Bone Morphogenetic Proteins/antagonists & inhibitors , Bone Morphogenetic Proteins/pharmacology , Cartilage/embryology , Embryonic Induction/drug effects , Fibroblast Growth Factors/pharmacology , Limb Buds/embryology , Proto-Oncogene Proteins/pharmacology , Animals , Apoptosis/physiology , Bone Morphogenetic Proteins/genetics , Cartilage/drug effects , Chick Embryo , Fibroblast Growth Factor 4 , Limb Buds/drug effects , Recombinant Proteins/pharmacology
8.
Glia ; 23(1): 11-23, 1998 May.
Article in English | MEDLINE | ID: mdl-9562181

ABSTRACT

Cell adhesion molecules (CAMs) must play a crucial role in both the initiation and signalling of axon-glial contact. However, the proteins that permit myelinating oligodendrocytes to recognize the axons that they ensheath in the developing CNS are unknown. By a subtractive cDNA library strategy, we have identified neurofascin as a powerful candidate for such a molecule. Neurofascin is strongly but transiently up-regulated in oligodendrocytes at the onset of myelinogenesis. Once oligodendrocytes have engaged their target axons the protein plays no further part, since the expression of the gene declines precipitously, in contrast to that of the major myelin component proteolipid protein, which remains elevated. After the initial surge of neurofascin expression in oligodendrocytes, there is a shift to a predominantly neuronal localization that persists into adulthood. Hence neurofascin in oligodendrocytes is unlikely to serve a function in the stabilization of the multilamellar sheath around the axon. The major neurofascin isoform of oligodendrocytes contains the third fibronectin type 3 (FNIII) repeat but lacks the mucin-like domain which supports the view that neurofascin isoforms are differentially expressed in the nervous system. Among the genes that are up-regulated during the terminal differentiation of the oligodendrocyte, neurofascin is unique in displaying a transient pattern of expression at the early stages of myelination. We propose that this CAM not only has a role in mediating axon recognition but also signals axonal contact through its links with the actin cytoskeleton.


Subject(s)
Axons/physiology , Brain/physiology , Cell Adhesion Molecules/biosynthesis , Myelin Sheath/physiology , Nerve Growth Factors/biosynthesis , Neuroglia/physiology , Oligodendroglia/physiology , Animals , Animals, Newborn , Brain/cytology , Cell Communication/physiology , Cells, Cultured , Gene Library , In Situ Hybridization, Fluorescence , Intercellular Junctions/physiology , Neuroglia/cytology , Oligodendroglia/cytology , RNA Probes , Rats , Rats, Wistar , Spinal Cord/cytology , Spinal Cord/physiology
9.
Br J Ind Med ; 36(4): 263-75, 1979 Nov.
Article in English | MEDLINE | ID: mdl-159718

ABSTRACT

The setting up of the Royal Commission on Civil Liability and Compensation for Personal Injury, its terms of reference and report are discussed. The main recommendations are detailed with brief consideration of their possible impact. The proposals are assessed and the philosophy and principles behind the reform, explicit or implicit in the Report, are considered. Finally, the extent to which the proposals can be a blueprint for later reforms dealing with compensation for disability in general is discussed.


Subject(s)
Occupational Diseases/economics , Workers' Compensation/legislation & jurisprudence , Accidents, Occupational/economics , Adult , Child , Disability Evaluation , Disabled Persons , Female , Financing, Organized , Humans , Male , Social Responsibility , Social Security , United Kingdom , Vaccination/adverse effects
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