First record of epizootic ulcerative syndrome from the Upper Congo catchment: An outbreak in the Bangweulu swamps, Zambia.

We report on the first outbreak of epizootic ulcerative syndrome (EUS) amongst wild fish populations in the Bangweulu swamps, an inland delta, in the north of Zambia during 2014. The area supports a large and diverse fish fauna related to, but distinct from, that of the Zambezi River system where EUS outbreaks have occurred since 2006. A sizeable artisanal fishery, based on extensive fish weirs, is sustained by the annual flooding of the swamps, and observations of the disease outbreak by fishermen were recorded. Signs typical of infection with Aphanomyces invadans were observed in a number of species. Clinical observations, histology and molecular diagnostic methods were used to confirm infection with A. invadans in two of the most commonly and severely affected species. Several features of the wetland may have contributed to the outbreak and the annual recurrence of the disease. Modes by which the disease may have been introduced into the swamps are discussed. The outbreak is of great significance as the Bangweulu swamps drain into the Congo River in neighbouring Democratic Republic of Congo, Africa's largest drainage system with an extensive and diverse fish fauna previously unaffected by EUS.

Bolo disease: a specific, localised skin disease of woolled sheep.

Bolo disease is limited to Merino and Döhne merino sheep in the Stutterheim and Cathcart districts of the eastern Cape Province. It occurs under natural grazing conditions regardless of the season of the year and the condition of the natural grazing. Ewes and wethers are most frequently affected. Skin lesions are well-defined, and the corresponding fleece has dark-grey to almost black spots, patches or bands varying in number, size and distribution between individual sheep. The wool in the affected areas is visibly shorter, less dense and tender, and the tips of the staples are spiky. In freshly-shorn sheep, the affected areas appear chalky white. Chronic and superimposed acute lesions are present in the same specimen histologically. Skin lesions include superficial and follicular hyperkeratosis, acanthosis, and sebaceous gland hyperplasia and hypertrophy. These changes are accompanied by dilatation of some of the follicles in the midshaft area, and collapse of the subepidermal tissue with only a few remaining collagen fibres separating the follicles and the sebaceous glands from the thickened epidermis. Corynebacterium spp. is the most consistent bacterium isolated from the lesions. Lesions produced by suspensions of this organism simulated both clinical signs and histopathological findings of the natural condition.

Bolo disease: a bacteriological survey.

A total of 718 sheep, 381 severely and 190 mildly affected with Bolo disease as well as 147 visibly unaffected animals emanating from 15 farms in the Stutterheim and Cathcart districts in the eastern Cape were subjected to bacteriological examination of skin surfaces and wool specimens. Altogether, 1,168 specimens were examined. These included skin swabs, skin scrapings and wool samples. Corynebacterium spp represented 94.4% of the primary isolates in cultures prepared from all specimens and 97.2% in those derived from skin swabs only, while a variety of other bacteria collectively constituted the remainder of primary isolates. In all, Corynebacterium spp was isolated from specimens of 94.2% of sheep severely affected with Bolo disease and from 83.7% of those mildly affected, whereas it could only be isolated from 1.36% clinically unaffected sheep. In a comparative study, swabs taken directly from the skin surface, proved to be the method of choice for the collection of specimens for bacteriological examination of Bolo disease. Using this method, Corynebacterium spp. was isolated from 98.7% of severely, and 85.3% of mildly affected sheep as well as 4% of sheep apparently unaffected by Bolo disease. The isolation of Corynebacterium spp. from skin scrapings collected from the 3 categories of affection (73.3%, 57.3% and 4% respectively) and from wool samples (52%, 41.3% and 1.3% respectively) proved these 2 methods of sampling to be less reliable. A close association has been established between the incidence of Corynebacterium spp. and the occurrence of clinical Bolo disease.

The starch digestion test in the horse.

Variable results were obtained when the starch digestion test was carried out on a suspected case of malassimilation in a horse. In order to re-evaluate this test, 15 starch digestion tests were carried out using 4 horses. Results showed a marked variation in the increase in plasma glucose levels between individual horses and especially between poor and good condition horses when the dose of starch was based on body mass. Results also suggest that a fixed dose of starch may give more consistent results.