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11.
Methods Find Exp Clin Pharmacol ; 20(4): 301-5, 1998 May.
Article in English | MEDLINE | ID: mdl-9658379

ABSTRACT

The basal peroxide production and the oxidative burst induced by phagocytosis of opsonized E. coli was studied by flow cytometry using dihydrorhodamine 123. The human leukocytes were incubated in the absence and presence of N-acetylcysteine. The oxidative response to the phagocytosis of bacteria differed among cell populations. Thus, 90% of granulocytes and 50% of monocytes showed an oxidative burst in response to opsonized bacteria while less than 1% of lymphocytes showed a fluorescence signal. N-Acetylcysteine (4.7, 9.5, 19, 38 or 76 mM) produced a dose-dependent inhibition of the oxidative response to phagocytosis in the three cellular populations reaching almost complete inhibition for 76 mM. This protective effect of N-acetylcysteine against oxidative stress in leukocytes was obtained without cytotoxicity (assessed by flow cytometry with staining with propidium iodide) or changes in the pH of the medium. These results give further support to the antioxidant effect of N-acetylcysteine in human peripheral blood cells.


Subject(s)
Acetylcysteine/pharmacology , Bacteria , Free Radical Scavengers/pharmacology , Leukocytes/drug effects , Leukocytes/physiology , Phagocytosis/drug effects , Phagocytosis/physiology , Respiratory Burst/drug effects , Cell Survival/drug effects , Flow Cytometry , Humans , Leukocytes/metabolism , Lymphocytes/drug effects , Lymphocytes/metabolism , Lymphocytes/physiology , Monocytes/drug effects , Monocytes/metabolism , Monocytes/physiology , Neutrophils/drug effects , Neutrophils/metabolism , Neutrophils/physiology , Oxidative Stress/physiology
12.
J Allergy Clin Immunol ; 98(1): 165-8, 1996 Jul.
Article in English | MEDLINE | ID: mdl-8765830

ABSTRACT

BACKGROUND: Previous experiments have shown that in vivo Fel d 1 production is under hormonal control. It strongly decreased 1 month after castration of male cats and increased after testosterone injection. OBJECTIVE: These results led us to put forward the hypothesis that Fel d 1 production could be more important in male that in female cats. Five adult male and five adult female cats were studied. METHODS: On day 0 three separate sites of each cat's trunk were shaved, and a 5.72 cm2 area in each site was washed twice (D0a and D0b) with 5 ml of distilled water. Then a collar was attached to the neck to prevent contamination from saliva. Twenty-four hours later on day 1, all areas were washed again once (D1a). Skin washes and fur extracts from the shaved areas were evaluated for Fel d 1 content. The same procedures (apart from collection of fur) were repeated 5 months later. RESULTS: The Fel d 1 level was higher in the first skin wash of male cats compared with that of female cats on each separate site of the trunk in both experiments, initially and 5 months later. Results were significant in the first experiment (median Fel d 1 per milliliter: 69.4 and 28.9 mU, respectively, for the combined three sites; p < 0.05). Fel d 1 production over a 24-hour period was higher in male cats, but the difference did not reach statistical significance. Furthermore, correlation between Fel d 1 levels in washes D0a and in fur was highly significant (p < 0.01). CONCLUSION: These results confirm that Fel d 1 originates from skin. Furthermore, they suggest that Fel d 1 production is higher in male than in female cats.


Subject(s)
Allergens/biosynthesis , Glycoproteins/biosynthesis , Sex Characteristics , Animals , Cats , Female , Hair/immunology , Male , Skin/immunology
15.
Sangre (Barc) ; 37(6): 429-33, 1992 Dec.
Article in Spanish | MEDLINE | ID: mdl-1293793

ABSTRACT

PURPOSE: To determine the incidence of a second malignancy in patients with Hodgkin's disease (HD) diagnosed and treated in the same hospital. PATIENTS AND METHODS: A retrospective study was performed on 99 patients diagnosed and treated for HD in the Hospital San Carlos, in Madrid, between January 1976 and december 1987. The clinical records were revised; the diagnosis and staging followed the Rye and Ann Arbor criteria, and the treatment included radiation therapy (RT), chemotherapy (CT), or a combination of both. The diagnosis of the second malignancy was based upon clinical, analytical, radiological and histological records. RESULTS: The median age in the series was 31 years (16-82) and the M/F ratio was 61/38. The stage distribution was: I-9; II-29; III-31, and IV-30. Twenty-six patients received RT alone, 59 were treated with CT, and 14 received RT plus CT. A second neoplasm was found in 6 patients (6%), of whom 4 developed a myelodysplastic syndrome (MDS) and 2 a solid tumour. All the patients who had MDS had received MOPP or C-MOPP chemotherapy, associated in two of them with extensive RT. Both patients with solid tumour had been given CT+RT. The median time of presentation of the second malignancy since the diagnosis od HD was 89 months (48-174) for MDS and 120 months for the solid tumours. The four MDS patients have died, 2 for ANLL-M5, one for SRA and the remainder for cerebral haemorrhage, not yet evolved into acute leukaemia. The two patients which solid tumours are alive and seemingly in complete remission at 12 and 10 months, respectively, of the diagnosis of the second malignancy. CONCLUSIONS: 1) All the patients with second neoplasms had been previously treated with CT (MOPP or C-MOPP) or CT+RT. 2) Non-Hodgkin's lymphoma has not appeared in any of the patients in this series. 3) An endless follow-up of patients with HD seems important in order to achieve an early diagnosis of other malignant complications which, although in case of MDS have poor prognosis, in case of solid tumours may do well with adequate treatment.


Subject(s)
Antineoplastic Combined Chemotherapy Protocols/adverse effects , Carcinoma, Papillary/etiology , Carcinoma, Squamous Cell/etiology , Hodgkin Disease/therapy , Leukemia, Monocytic, Acute/etiology , Lung Neoplasms/etiology , Myelodysplastic Syndromes/etiology , Neoplasms, Radiation-Induced/epidemiology , Neoplasms, Second Primary/etiology , Radiotherapy/adverse effects , Thyroid Neoplasms/etiology , Adolescent , Adult , Aged , Aged, 80 and over , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Cyclophosphamide/administration & dosage , Cyclophosphamide/adverse effects , Female , Hodgkin Disease/drug therapy , Hodgkin Disease/radiotherapy , Humans , Incidence , Male , Mechlorethamine/administration & dosage , Mechlorethamine/adverse effects , Middle Aged , Neoplasms, Radiation-Induced/etiology , Neoplasms, Second Primary/epidemiology , Prednisone/administration & dosage , Prednisone/adverse effects , Procarbazine/administration & dosage , Procarbazine/adverse effects , Retrospective Studies , Vincristine/administration & dosage , Vincristine/adverse effects
16.
Clin Immunol Immunopathol ; 60(1): 65-71, 1991 Jul.
Article in English | MEDLINE | ID: mdl-2044238

ABSTRACT

We describe the presence of IgG antibodies reacting with histones previously treated with proteases in a patient with vasculitis. The patient's serum did not react with nontreated histones and when several enzymes were tested separately, only alpha-chymotrypsin reproduced the effect. Reactivity was directed against histone fraction H2B and no other autoantibody was found in the patient's serum. This could represent an autoantigen-driven response, histones hydrolyzed in vivo with proteases being the immunogenic stimulus. Diagnostic and pathogenic implications derived from the existence of such autoantibodies are discussed.


Subject(s)
Autoantibodies/analysis , Endopeptidases/pharmacology , Histones/immunology , Vasculitis/immunology , Adult , Autoimmune Diseases/etiology , Humans , Male
17.
Am J Public Health ; 77(9): 1207-9, 1987 Sep.
Article in English | MEDLINE | ID: mdl-3618856

ABSTRACT

An elective cancer education course at a public university attracted twice as many women as men. Course information was disseminated to an average of eight people by each student. The course seemed reasonably effective in stimulating positive behavioral changes. According to self-report, dietary fat was reduced by 47 per cent of women and 49 per cent of men. Fiber intake was increased by 48 per cent of women and 36 per cent of men. Breast self-examination was initiated by 36 per cent of previous nonperformers: and testicular self-examination, by 23 per cent.


Subject(s)
Health Education , Neoplasms , Public Health , Breast , California , Curriculum , Diet , Female , Humans , Male , Neoplasms/prevention & control , Palpation , Students , Testis , Universities
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