ABSTRACT
A Tris-citric acid-glucose (TCG) diluent containing low concentrations (6%) of egg yolk, and a TCG extender containing lactose (without egg yolk), were compared for use in the cryopreservation of Spanish ibex (Capra pyrenaica) epididymal spermatozoa. To optimize the collection of epididymal spermatozoa, two spermatozoa recovery methods were tested: i) by using small cuts in the cauda epididymides and ii) by the application of air pressure (from a syringe) inside the vas deferens. The percentage of viable spermatozoa recovered was lower (P < 0.05) with the air pressure method. No significant differences were seen in the efficacy of the two diluents as determined by percentage viability of thawed sperm, membrane integrity (as determined by the hypo-osmotic swelling test), or acrosome integrity. The use of the TCG-lactose medium strongly reduced sperm motility (P < 0.001). The sperm samples that had been diluted with TCG-6% egg yolk extender showed a greater incidence (P < 0.05) of morphological abnormalities. TCG-lactose alone, does not well preserve motility when cryopreserving Spanish ibex epididymal spermatozoa.
Subject(s)
Epididymis , Goats , Semen Preservation/methods , Spermatozoa/cytology , Acrosome/ultrastructure , Animals , Cryopreservation , Epididymis/cytology , Functional Laterality , Male , Spain , Sperm Motility , Testis/anatomy & histologyABSTRACT
La infección por Mycobacterium tuberculosis, es un diagnóstico de exclusión que siempre debe estar presente en todos los casos de derrame pericárdico agudo y crónico, más aún en nuestro país donde la prevalencia de tuberculosis es cada vez más alta. El diagnóstico precoz y el inicio del tratamiento influyen directamente en la sobrevida del paciente y su mortalidad. Aquí presentamos 4 pacientes con diagnóstico de pericarditis tuberculosa quienes tuvieron diferente evolución y grados variables de derrame.
Mycobacterium tuberculosis infection is a diagnosis of exclusion that must always be present in all cases of acute and chronic pericardial effusion, even more so in our country where the prevalence of tuberculosis is increasingly high. Early diagnosis and initiation of treatment directly influence patient survival and mortality. Here we present 4 patients with a diagnosis of tuberculous pericarditis who had different evolution and variable degrees of effusion.
Subject(s)
Humans , Male , Female , Adolescent , Middle Aged , Pericardial Effusion , Pericarditis, Tuberculous , Mycobacterium tuberculosis , Therapeutics , Public Health , Early DiagnosisABSTRACT
La tuberculosis es un problema de salud pública a nivel mundial. La localización extrapulmonar a nivel mamario es rara y sin manifestaciones sistémicas. Clínicamente es indistinguible de las neoplasias de la glándula, esto dificulta el diagnóstico y retarda el incio del tratamiento. Presentamos dos casos de tuberculosis mamaria, además se revisa la literatura sobre el tema e insistimos sobre el problema diagnóstico.
