The role epiphytes play in particle capture of seagrass canopies.

Seagrass epiphytic communities act as ecological indicators of the quality status of vegetated coastal environments. This study aims to determine the effect leaf epiphytes has on the sediment capture and distribution from outside sources. Thirteen laboratory experiments were conducted under a wave frequency of 0.5 Hz. Three epiphyte models were attached to a Zostera marina canopy of 100 plants/m2 density. The sediment deposited to the seabed, captured by the epiphytic leaf surface, and remaining in suspension within the canopy were quantified. This study demonstrated that the amount of epiphytes impacts on the sediment stocks. Zostera marina canopies with high epiphytic areas and long effective leaf heights may increase the sediment captured on the epiphyte surfaces. Also, reducing suspended sediment and increasing the deposition to the seabed, therefore enhancing the clarity of the water column. For largest epiphytic areas, a 34.5% increase of captured sediment mass is observed. The sediment trapped on the leaves can be 10 times greater for canopies with the highest epiphytic areas than those without epiphytes. Therefore, both the effective leaf length and the level of epiphytic colonization are found to determine the seagrass canopy ability at distributing sediment.

SARS-CoV-2 infection in children with cystic fibrosis: A cross-sectional multicenter study in Spain. New waves, new knowledge.

INTRODUCTION: The association between viral infections and pulmonary exacerbations in children with cystic fibrosis (cwCF) is well established. However, the question of whether cwCF are at a higher risk of COVID-19 or its adverse consequences remains controversial. METHODS: We conducted an observational, multicenter, cross-sectional study of cwCF infected by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) between March 2020 and June 2022, (first to sixth COVID-19 pandemic waves) in Spain. The study aimed to describe patients' basal characteristics, SARS-CoV-2 clinical manifestations and outcomes, and whether there were differences across the pandemic waves. RESULTS: During study time, 351 SARS-CoV2 infections were reported among 341 cwCF. Median age was 8.5 years (range 0-17) and 51% were female. Cases were unevenly distributed across the pandemic, with most cases (82%) clustered between November 2021 and June 2022 (sixth wave, also known as Omicron Wave due to the higher prevalence of this strain in that period in Spain). Most cwCF were asymptomatic (24.8%) or presented with mild Covid-19 symptoms (72.9%). Among symptomatic, most prevalent symptoms were fever (62%) and increased cough (53%). Infection occurring along the sixth wave was the only independent risk factor for being symptomatic. Just eight cwCF needed hospital admission. No multisystem inflammatory syndrome, persisting symptoms, long-term sequelae, or deaths were reported. CONCLUSIONS: Spanish current data indicate that cwCF do not experience higher risks of SARS-CoV-2 infection nor worse health outcomes or sequelae. Changes in patients' basal characteristics, clinical courses, and outcomes were detected across waves. While the pandemic continues, a worldwide monitoring of COVID-19 in pediatric CF patients is needed.

Suspended sediments mediate microplastic sedimentation in unidirectional flows.

Microplastic particles (MP) are an emerging contaminant threatening many aquatic systems. Because of the sharp increase in plastic manufacture, the concentration of MP in natural ecosystems has grown dramatically. While it is known that when MP enter aquatic ecosystems they are transported and dispersed via different mechanisms (currents, waves, turbulence), the processes involved are still poorly understood. In the current study, the transport of MP by a unidirectional flow has been investigated in a laboratory flume. MP enter the system through a plume that can (or not) have suspended sediment. The interaction between MP and sediment was studied for three different MP particle types (Polyamide (PA) and Polyvinyl Chloride (PVC) fragments, and Polyethylene Terephthalate (PET) fibers), and four different sediment concentrations (0 g/l, 15 g/l, 30 g/l and 45 g/l). In all cases, sediment increased the vertical transport of MP to the bottom. The greater the sediment concentration, the greater the downward flux of MP. Sediment particles scavenged PA fragments downwards at the highest rate, followed by PET fibers and finally PVC fragments. These results indicate that a sediment particle-laden plume carrying MP may induce a differential settling of MP as they are advected. The scavenging of MP by sediments may result in sedimentation segregated patterns, with MP being found at shorter distances than expected for the case without sediment, therefore increasing the presence of MP near their contaminant sources.

Spatial sedimentation and plant captured sediment within seagrass patches.

Habitat degradation in coastal ecosystems has resulted in the fragmentation of coastal aquatic vegetation and compromised their role in supplying essential ecological services such as trapping sediment or sequestering carbon. Fragmentation has changed seagrass architecture by decreasing the density of the canopy or engendering small patches of vegetated areas. This study aims to quantify the role different patch sizes of vegetation with different canopy densities have in the spatial distribution of sediment within a patch. To this aim, two canopy densities, four different patch lengths, and two wave frequencies were considered. The amounts of sediment deposited onto the bed, captured by plant leaves, remaining in suspension within the canopy, and remaining in suspension above the canopy were used to understand the impact hydrodynamics has on sediment distribution patterns within seagrass patches. In all the cases studied, patches reduced the suspended sediment concentrations, increased the capture of particles in the leaves, and increased the sedimentation rates to the bed. For the lowest wave frequency studied (0.5 Hz), the sediment deposited to the bottom was enhanced at canopy edges, resulting in spatial heterogeneous sedimentation patterns. Therefore, restoration and preservation of coastal aquatic vegetation landscapes can help face future climate change scenarios where an increase in sedimentation can help mitigate predicted sea level rise in coastal areas.

Stem stiffness functionality in a submerged canopy patch under oscillatory flow.

Seagrass canopies are coastal ecosystems that are able to modify the abiotic environment through their architectural structure. They have different structural parameters, such as plant stem stiffness, patch length and canopy density, all of which determine their overall functionality in modifying the seafloor hydrodynamics within coastal areas. To determine the interaction between hydrodynamics and the canopy structure, a set of laboratory experiments were carried out with both rigid and flexible stems for different canopy densities, patch lengths and wave frequencies. In the upper part of the canopy, flexible plants move with the flow without generating drag or producing turbulent kinetic energy, while rigid plants generate drag and produce turbulent kinetic energy. In the inner canopy layer, both types of plants behave like rigid stems and produce turbulent kinetic energy. A non-dimensional model based on the turbulent kinetic energy, the wave velocity and the plant characteristics is presented to describe the behaviour of flexible and rigid plants under an oscillating flow. Flexible plants behave in a stiffer manner under high wave frequencies than under low wave frequencies, thus making their behaviour closer to that of rigid plant stems. This difference between both canopy structures can explain their distribution in the environment, with rigid canopies being more extended in more sheltered regions while flexible plants are characteristic of more exposed regions with high flow energy.

I-DTI, a Second-Opinion Platform Related to Organ Donation and Transplant Between Health Care Professionals.

OBJECTIVES: The COVID-19 pandemic led to a decline in donation and transplant programs worldwide. Telehealth was explored as a strategy to continue organ procurement activity. The aim of this project was to develop and test I-DTI, an online medical platform for health care professionals specialized in the field of organ donation and transplant, that provides second-opinion consultancy and instant-messaging services. MATERIALS AND METHODS: The Donation and Transplantation Institute (DTI Foundation), in collaboration with the developers of an operative communication engine (Medxat/Be-Hit), designed the I-DTI platform, via a web-based application. I-DTI contents were created by the DTI Foundation medical team and international experts in organ donation and transplantation. I-DTI was launched in 2020 in a 6-month pilot phase, in which hospitals from India (Kerala), Philippines, Trinidad and Tobago, and Sri Lanka were included. In the pilot phase, about 60 health care professionals were involved and >20 international experts were enrolled to respond to incoming inquiries. The following topics were considered for this review: organ donation, organ transplantation, transplant follow-up, tissue donation, and COVID-19. Data collected were entered anonymously into an encrypted database for academic purposes. A survey was then conducted for all users to improve its acceptance and feasibility. RESULTS: On average, the second-opinion service was consulted 2 times per week by the participants, and experts' opinions were delivered in <24 hours. An intuitive user interface led participants to use the messaging service daily. Active dissemination contributed to I-DTI growth, achieving 300 users from >20 countries within the first year. CONCLUSIONS: I-DTI has proved to be a feasible tool to support health care professionals, for knowledge exchange and communication, ensuring access to international best practices. Nevertheless, it is imperative that medical providers actively encourage the use of innovative solutions available, especially in the areas with restricted access to knowledge.

Incidence and Prevalence of Children's Diffuse Lung Disease in Spain.

BACKGROUND: Children's diffuse lung disease, also known as children's Interstitial Lung Diseases (chILD), are a heterogeneous group of rare diseases with relevant morbidity and mortality, which diagnosis and classification are very complex. Epidemiological data are scarce. The aim of this study was to analyse incidence and prevalence of chILD in Spain. METHODS: Multicentre observational prospective study in patients from 0 to 18 years of age with chILD to analyse its incidence and prevalence in Spain, based on data reported in 2018 and 2019. RESULTS: A total of 381 cases with chILD were notified from 51 paediatric pulmonology units all over Spain, covering the 91.7% of the paediatric population. The average incidence of chILD was 8.18 (CI 95% 6.28-10.48) new cases/million of children per year. The average prevalence of chILD was 46.53 (CI 95% 41.81-51.62) cases/million of children. The age group with the highest prevalence were children under 1 year of age. Different types of disorders were seen in children 2-18 years of age compared with children 0-2 years of age. Most frequent cases were: primary pulmonary interstitial glycogenosis in neonates (17/65), neuroendocrine cell hyperplasia of infancy in infants from 1 to 12 months (44/144), idiopathic pulmonary haemosiderosis in children from 1 to 5 years old (13/74), hypersensitivity pneumonitis in children from 5 to 10 years old (9/51), and scleroderma in older than 10 years old (8/47). CONCLUSIONS: We found a higher incidence and prevalence of chILD than previously described probably due to greater understanding and increased clinician awareness of these rare diseases.

Incidence and Prevalence of Children's Diffuse Lung Disease in Spain / Incidencia y prevalencia de las neumopatías intersticiales pediátricas en España

Background Children's diffuse lung disease, also known as children's Interstitial Lung Diseases (chILD), are a heterogeneous group of rare diseases with relevant morbidity and mortality, which diagnosis and classification are very complex. Epidemiological data are scarce. The aim of this study was to analyse incidence and prevalence of chILD in Spain. Methods Multicentre observational prospective study in patients from 0 to 18 years of age with chILD to analyse its incidence and prevalence in Spain, based on data reported in 2018 and 2019. Results A total of 381 cases with chILD were notified from 51 paediatric pulmonology units all over Spain, covering the 91.7% of the paediatric population. The average incidence of chILD was 8.18 (CI 95% 6.28–10.48) new cases/million of children per year. The average prevalence of chILD was 46.53 (CI 95% 41.81–51.62) cases/million of children. The age group with the highest prevalence were children under 1 year of age. Different types of disorders were seen in children 2–18 years of age compared with children 0–2 years of age. Most frequent cases were: primary pulmonary interstitial glycogenosis in neonates (17/65), neuroendocrine cell hyperplasia of infancy in infants from 1 to 12 months (44/144), idiopathic pulmonary haemosiderosis in children from 1 to 5 years old (13/74), hypersensitivity pneumonitis in children from 5 to 10 years old (9/51), and scleroderma in older than 10 years old (8/47). Conclusions We found a higher incidence and prevalence of chILD than previously described probably due to greater understanding and increased clinician awareness of these rare diseases.

Antecedentes Las neumopatías intersticiales pediátricas, también conocidas con el acrónimo chILD (del inglés children's Interstitial Lung Diseases), es un grupo heterogéneo de enfermedades raras con morbimortalidad relevante, cuyo diagnóstico y clasificación son complejos. Los estudios epidemiológicos son escasos. El objetivo de este trabajo fue analizar la incidencia y la prevalencia de chILD en España. Métodos Estudio prospectivo observacional multicéntrico en pacientes de 0 a 18 años afectos de chILD para analizar la incidencia y la prevalencia en España, a partir de datos recogidos en 2018 y 2019. Resultados Se recogieron 381 casos de chILD entre 51 unidades de neumología pediátrica de toda España, que cubrían el 91,7% de la población pediátrica. La incidencia promedio fue 8,18 (IC 95%: 6,28-10,48) casos nuevos/millón de niños por año. La prevalencia promedio fue de 46,53 (IC 95%: 41,81-51,62) casos/millón de niños. El grupo de edad con mayor prevalencia fue el de niños menores de un año. Se observaron diferentes entidades en niños de 2 a 18 años en comparación con niños de 0 a 2 años. Los diagnósticos más frecuentes fueron: glucogenosis intersticial pulmonar primaria en neonatos (17/65), hiperplasia de células neuroendocrinas en lactantes de uno a 12 meses (44/144), hemosiderosis pulmonar idiopática en niños de uno a 5 años (13/74), neumonía por hipersensibilidad en niños de 5 a 10 años (9/51) y esclerodermia en mayores de 10 años (8/47). Conclusiones Encontramos una mayor incidencia y prevalencia de chILD que las descritas previamente, probablemente debido a un mayor conocimiento y detección de estas enfermedades raras.

Vertical distribution of microplastics in water bodies causes sublethal effects and changes in Daphnia magna swimming behaviour.

Plastic debris has been found to be ubiquitous in many aquatic ecosystems and is constantly accumulating, not only because more and more plastic is being rapidly released into the environment, but also because its slow degradation means it persists in the water. Some more buoyant plastics accumulate in the water column, whereas other heavier types sink to the bottom. Consequently, the presence of microplastics can threaten organisms living in the water column as well as those found in the benthic zone. In this study, the filter feeder Daphnia has been found to ingest microplastics as the particle diameter (< 30 µm) is within their edible particle size range and they are unable to differentiate between particles of different natures. Four different treatments were considered: food only; only microplastic particles; 50% food and 50% microplastic particles; neither food nor microplastics. Sinking microplastics have been found to decrease Daphnia magna individuals' swimming velocity during vertical or cruising swimming trajectories, therefore demonstrating the sublethal effects microplastics have on this organism. In addition, microplastics decreased their body growth and survival rates. In cases with the presence of only microplastics, the swimming trajectories of Daphnia indicated the most serious stress experienced as individuals reversed vertical or cruising swimming trajectories to hopping and sinking movements. Therefore, Daphnia individuals in freshwater systems polluted by microplastics might take on the role of ingesting them and later on transporting them to deeper layer water column. In this way microplastics that would remain in the water column for a long time due to their buoyancy, might accumulate at the bottom of the water column.

Functional dynamics of vegetated model patches: The minimum patch size effect for canopy restoration.

For the past two centuries coastal zones have been suffering seagrass loss resulting in a network of vegetated patches which are barely interconnected and which may compromise the ecological services provided by the canopy. To optimize management efforts for successful restoration strategies, questions need to be addressed about what appropriate canopy architectural considerations are required under certain hydrodynamic conditions. In this study, a set of laboratory experiments were conducted in which hydrodynamic conditions, plant densities and vegetated patch lengths were varied to determine minimum patch lengths for successful management strategies. Based on the TKE production, this study finds two possible canopy behaviours of seagrasses under oscillating flows: one where plants do not interact with the flow and the other where they interact with waves and produce TKE. A threshold from the first to second behaviour occurs for [Formula: see text] = 2, where CD is the drag of the vegetated patch, n is the number of stems per m2, d is the stem diameter and ϕ is the solid plant fraction. Therefore, high canopy densities, large patches of vegetation or moderate wave velocities will produce plant-wave interaction, whereas low canopy densities, small vegetation patches or slow wave velocities will produce a behaviour akin to the non-vegetated cases.

Respiratory outcomes of "new" bronchopulmonary dysplasia in adolescents: A multicenter study.

OBJECTIVE: Long-term respiratory consequences of bronchopulmonary dysplasia (BPD) in preterm infants born in the post-surfactant era ("new" BPD) remain partially unknown. The present study aimed to evaluate the respiratory outcomes of "new" BPD in adolescents who were born preterm. METHODS: This multicenter, cross-sectional study included 286 adolescents born between 2003 and 2005 (mean age: 14.2 years); among them, 184 and 102 were born extremely preterm (EP; <28 weeks' gestation) and moderate-late preterm (32 to <37 weeks' gestation), respectively. Among EP adolescents, 92 had BPD, and 92 did not. All participants underwent lung function tests, skin prick testing, and questionnaires on asthma symptoms and quality of life. RESULTS: EP adolescents with BPD had significantly lower forced expiratory volume in 1 s (FEV1 ), forced vital capacity (FVC), FEV1 /FVC ratio, and forced expiratory flow between 25% and 75% of FVC than other included adolescents. FEV1 /FVC ratios were below the lower limit of normal (z-score <-1.645) in 30.4% of EP adolescents with BPD, 13.0% of EP adolescents without BPD, and 11.8% of adolescents who were born moderate-late preterm. Bronchodilator response and air-trapping were significantly higher in BPD adolescents than in other adolescents. Diffusion capacity was significantly lower in EP adolescents than in moderate-late preterm adolescents. Asthma symptoms and quality-of-life scores were similar among groups. CONCLUSION: EP adolescents with "new" BPD had poorer pulmonary function than EP adolescents without BPD or moderate-late preterm adolescents. Further studies are needed to determine whether "new" BPD is associated with early-onset chronic obstructive pulmonary disease in adulthood.

Zooplankton-based reactors for tertiary wastewater treatment: A pilot-scale case study.

Nature-based wastewater treatments are an economic and sustainable alternative to intensive technologies in rural areas, although their efficiency needs to be improved. This study explores technological co-operation between zooplankton (e.g., Daphnia magna) and bacterial and algal biofilms in a 1.5 m3 zooplankton-based reactor for the on-site treatment of secondary urban wastewater. The efficiency of the reactor was evaluated over a 14-month period without any maintenance. The results suggest a low seasonality effect on nutrient polishing (organic matter and nitrogen) and the removal of solids (TSS and turbidity). The best performance, involving a decrease in organic carbon, nitrogen, E. coli loads, and solid content was achieved in winter when operating the reactor at 750 L d-1. Under these conditions, the quality of the effluent water was suitable for its reuse for six different purposes in conformance with Spanish legislation. These results demonstrate that the zooplankton-based reactor presented here can be used as an eco-sustainable tertiary treatment to provide water suitable for reuse. On-site research revealed that the robustness of the reactor against temperature and oxygen fluctuations needs to be improved to ensure good performance throughout the year.

[50 years of the Neonatal Screening Program in Catalonia.] / 50 años del Programa de Cribado Neonatal en Cataluña.

The Catalonian Newborn Screening Program (CNSP) began in 1969, in Barcelona. It was promoted by Dr. Juan Sabater Tobella and supported by Barcelona Provincial Council and Juan March Foundation. That is how the Institute of Clinical Biochemistry was born, whose aims were diagnosis, research and teaching, along with the spirit of contributing to the prevention of mental retardation. The CNSP began with the detection of phenylketonuria (PKU), and, in 1982, the Program was expanded with the inclusion of congenital hypothyroidism detection. Towards 1990, the Program covered almost 100% of all newborns (NB) in Catalonia. In 1999, the CNSP was expanded with the incorporation of cystic fibrosis. It took fourteen years, until 2013, to make the largest expansion so far, with the incorporation of 19 metabolic diseases to the screening panel. The detection of sickle cell disease began in 2015 and in 2017 the detection of severe combined immunodeficiency was included. Currently, the CNSP includes 24 diseases in its main panel. Since 1969, 2,787,807 NBs have been screened, of whom 1,724 have been diagnosed with any of these diseases, and 252 of other disorders by differential diagnosis with those included in the main panel. The global prevalence is 1: 1,617 NBs affected by any of the diseases included in the CNSP and 1: 1,140 NBs if incidental findings diagnosed through the CNSP are included.

El Programa de Cribado Neonatal de Cataluña (PCNC) se inició en el año 1969, en Barcelona, impulsado por el Dr. Juan Sabater Tobella y apoyado por la Diputación de Barcelona y la Fundación Juan March. Así nació el Instituto de Bioquímica Clínica para acometer funciones asistenciales, de investigación y docencia, con el espíritu de contribuir a la prevención del retraso mental. El PCNC se inició con la detección de la fenilcetonuria (PKU) y en el año 1982 se amplió con la detección del hipotiroidismo congénito. Hacia el año 1990 la cobertura territorial llegó casi al 100% de todos los recién nacidos en Cataluña. En 1999 se amplió el PCNC con la incorporación de la fibrosis quística y tras catorce años, en 2013, se realizó la ampliación más numerosa hasta ahora, con la incorporación de la detección de 19 enfermedades metabólicas hereditarias. En el año 2015 comenzó la detección de la enfermedad de células falciformes y en el 2017 la detección de la inmunodeficiencia combinada grave. Actualmente, el PCNC incluye la detección de 24 enfermedades. Desde su inicio en el año 1969, se han cribado 2.787.807 recién nacidos, de los cuales 1.724 han sido diagnosticados de alguna de las 24 enfermedades que componen nuestro panel principal y 252 por diagnóstico diferencial de las primeras. En total la prevalencia global es de 1:1.617 RN afectos de alguna de las enfermedades incluidas en el PCNC y de 1:1.140 RN si se incluyen los hallazgos incidentales encontrados.

Synergistic effects of water temperature, microplastics and ammonium as second and third order stressors on Daphnia magna.

Daphnids, including the water flea Daphnia magna, can be exploited for wastewater treatment purposes, given that they are filter feeder organisms that are able to remove suspended particles from water. The presence of pollutants, such as microplastics and chemicals, might be considered stressors and modify the behaviour and survival of D. magna individuals. The impact of the cumulative pollutants that regulate the fate of living organisms has yet to be fully determined. Here we present the effect of double and triple combinations of stressors on the behaviour of D. magna. The impact of water temperature, ammonium and polystyrene microplastics on the filtration capacity and survival of D. magna is studied. Water temperatures of 15 °C, 20 °C and 25 °C, microplastic-to-food ratios of 25% and 75%, and ammonium concentrations of 10 and 30 mg N-NH4+ L-1 are tested after making dual and triple combinations of the parameters. A synergistic effect between water temperature and ammonium is normally observed but not in the case of the lower values of ammonium concentration and temperature. The combination of three stressors (water temperature, microplastics and ammonium) is also found to be synergistic, producing the greatest impact on D. magna filtration capacity and reducing their survival. In comparison with the effect of the two stressor conditions, the combination of the three stressors caused a reduction of between 13.1% and 91.7% in the t50% time (the time required for a 50% reduction in the D. magna filtration capacity) and a reduction of between 4.8% and 54.5% in TD50 (the time for 50% mortality).

Assessment of zooplankton-based eco-sustainable wastewater treatment at laboratory scale.

The combination of the filtration capacity of zooplankton (e.g. Daphnia) with the nutrient removal capacity of bacterial/algal biofilm in a zooplankton-containing reactor could provide a natural-based alternative for wastewater treatment. A laboratory-scale zooplankton-based reactor was tested at different HRTs resulting in a significant reduction in nutrient concentrations in wastewater when the system was operated at HRTs longer than 1.1 days (preferably of between 2 and 4 days). However, the presence of high concentrations of organic matter (>250 mg COD L-1) in the wastewater inhibited zooplankton activity, limiting its use to tertiary treatment. Therefore, in combination with other natural treatments that can perform primary and secondary treatments, zooplankton may provide a solution for wastewater clarification and nutrient polishing. The effect of a common metal such as copper on the filtration capacity of Daphnia was also evaluated. Daphnia, as well as the whole zooplankton-based reactor, adapted to copper concentrations of up to 70 µg Cu L-1 but an overload of 380 µg Cu L-1 for two-weeks severely affected the biological system.

Particle size segregation of turbidity current deposits in vegetated canopies.

Interactions between ecology, hydrodynamics and sediments play central roles in the evolution of coastal and freshwater ecosystems. We set out to characterise interactions of a specific hydrodynamic phenomenon - turbidity currents - with vegetation and sediment dynamics. We measured hydrodynamics and sediment deposition rates when turbidity currents flowed into plant canopies in a lock-exchange flume experiment, using simulated vegetation and three real plant species, and varying the turbidity current's initial sediment concentration. The natural sediment used had an essentially bimodal size distribution, with coarse (6.2-104 µm) and fine (2.2-6.2 µm) fractions. In all cases, on entering the vegetation canopy, the turbidity current was initially inertially-dominated, but subsequently became drag-dominated. In the inertial regime, there was no size segregation in the deposited material. In the drag-dominated regime, the deposited material became increasingly dominated by fine sediment, at a rate dependent on the vegetation type. The transition between these two regimes occurred at a distance equivalent to 5.1-7.6 times the total water depth downstream of the lock gate. The size segregation of deposited sediment is posited to have consequences for substrate evolution, which in turn may affect vegetation growth. Thus, our findings point to a non-linear feedback mechanism between the spatial heterogeneity of vegetation canopies and that of the substrate they help to engineer.

50 años del Programa de Cribado Neonatal en Cataluña / 50 years of the Neonatal Screening Program in Catalonia

El Programa de Cribado Neonatal de Cataluña (PCNC) se inició en el año 1969, en Barcelona, impulsado por el Dr. Juan Sabater Tobella y apoyado por la Diputación de Barcelona y la Fundación Juan March. Así nació el Instituto de Bioquímica Clínica para acometer funciones asistenciales, de investigación y docencia, con el espíritu de contribuir a la prevención del retraso mental. El PCNC se inició con la detección de la fenilcetonuria (PKU) y en el año 1982 se amplió con la detección del hipotiroidismo congénito. Hacia el año 1990 la cobertura territorial llegó casi al 100% de todos los recién nacidos en Cataluña. En 1999 se amplió el PCNC con la incorporación de la fibrosis quística y tras catorce años, en 2013, se realizó la ampliación más numerosa hasta ahora, con la incorporación de la detección de 19 enfermedades metabólicas hereditarias. En el año 2015 comenzó la detección de la enfermedad de células falciformes y en el 2017 la detección de la inmunodeficiencia combinada grave. Actualmente, el PCNC incluye la detección de 24 enfermedades. Desde su inicio en el año 1969, se han cribado 2.787.807 recién nacidos, de los cuales 1.724 han sido diagnosticados de alguna de las 24 enfermedades que componen nuestro panel principal y 252 por diagnóstico diferencial de las primeras. En total la prevalencia global es de 1:1.617 RN afectos de alguna de las enfermedades incluidas en el PCNC y de 1:1.140 RN si se incluyen los hallazgos incidentales encontrados

The Catalonian Newborn Screening Program (CNSP) began in 1969, in Barcelona. It was promoted by Dr. Juan Sabater Tobella and supported by Barcelona Provincial Council and Juan March Foundation. That is how the Institute of Clinical Biochemistry was born, whose aims were diagnosis, research and teaching, along with the spirit of contributing to the prevention of mental retardation. The CNSP began with the detection of phenylketonuria (PKU), and, in 1982, the Program was expanded with the inclusion of congenital hypothyroidism detection. Towards 1990, the Program covered almost 100% of all newborns (NB) in Catalonia. In 1999, the CNSP was expanded with the incorporation of cystic fibrosis. It took fourteen years, until 2013, to make the largest expansion so far, with the incorporation of 19 metabolic diseases to the screening panel. The detection of sickle cell disease began in 2015 and in 2017 the detection of severe combined immunodeficiency was included. Currently, the CNSP includes 24 diseases in its main panel. Since 1969, 2,787,807 NBs have been screened, of whom 1,724 have been diagnosed with any of these diseases, and 252 of other disorders by differential diagnosis with those included in the main panel. The global prevalence is 1: 1,617 NBs affected by any of the diseases included in the CNSP and 1: 1,140 NBs if incidental findings diagnosed through the CNSP are included

Optimal light conditions for Daphnia filtration.

Daphnia populations are present in lakes and ponds. They are known to experience diurnal vertical migrations according to their feeding needs. During the day they migrate downwards to avoid predation in light-receiving layers and at night they migrate upwards, searching for food in the shallow productive layers. The light photoperiod and light intensity vary depending on the latitude and, therefore, the precise location of lakes and ponds will be an additional and crucial parameter in determining the development of Daphnia. Here we will focus on a population of Daphnia magna (a genus of the Cladocera order). The effect of both light intensity and photoperiod on Daphnia filtration was studied in laboratory experiments. An increase in the light intensity resulted in two D. magna responses depending on the exposure time of individuals to light. Short time exposures to a decrease in the light intensity of less than one day produced an increase in the D. magna filtration. However, exposures of longer than one day resulted in a decrease in the D. magna filtration along with a decrease in the light intensity. Photoperiod exposures of 8, 12 and 16 h produced greater D. magna filtrations than photoperiods of 0, 4 and 24 h. In this study, regulation of the light intensity and the period of exposure were used in laboratory experiments to establish D. magna development thresholds by latitudinal variation in the photoperiod.

Mediated food and hydrodynamics on the ingestion of microplastics by Daphnia magna.

There is consensus on the need to study the potential impact microplastics (MP) have on freshwater planktonic organisms. It is not yet fully understood how MP enter the aquatic food web or the effect they have on all the trophic levels. As a result of the potential for MP to accumulate throughout food webs, there is increasing interest in evaluating their fate in a variety of environmental conditions. This study investigated the variability in the ingestion of MP to food ratios and the exposed time of MP to Daphnia magna in non-sheared and sheared conditions. The sheared environment provided Daphnia magna with the conditions for optimal filtering capacity. Regardless of the ratios of MP concentration to food concentration (MP:Food), the filtration capacity of the Daphnia magna was enhanced in the sheared experiments. In both the sheared and non-sheared experiments, filtration capacity decreased when the ratios of MP to food concentration and the exposure times to MP were increased. Mortality was mainly enhanced in the non-sheared conditions at higher MP concentrations and exposure times to MP. No mortality was found in the sheared conditions for the exposure times studied. Therefore, in aquatic systems that undergo constant low sheared conditions, Daphnia magna can survive longer when exposed to MP than in calm conditions, provided food concentrations do not limit their capacity to filter.

Teachers' involvement and students' self-efficacy: Keys to achievement in higher education.

We study the relative importance of the three dimensions of need-supportive teaching (NST) and students' self-efficacy to gain new knowledge about students' achievement in higher education. NST assumes that teachers are key to the motivation of students, providing autonomy support, structure (support of competence), and involvement (support of relatedness). In turn, self-efficacy raises students' confidence in their ability to succeed in academic tasks. Drawing on 86,000 records of teaching evaluations by students at the University of Girona (Spain), we present evidence that teachers' involvement and students' self-efficacy are the two elements most strongly and positively related to achievement. Students obtain higher marks when they believe that their teachers are dependable and available to offer resources, and when they feel capable of organizing and implementing the courses of action necessary to acquire knowledge. We also find that students' experience of autonomy support and structure are negatively (or not) correlated with achievement. Subgroup analyses also indicate that students have different needs in different knowledge areas.